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BMC Infectious Diseases
Published in: BMC Infectious Diseases 1/2013

Open Access 01-12-2013 | Research article

CCL3L1 copy number, HIV load, and immune reconstitution in sub-Saharan Africans

Authors: Eleni Aklillu, Linda Odenthal-Hesse, Jennifer Bowdrey, Abiy Habtewold, Eliford Ngaimisi, Getnet Yimer, Wondwossen Amogne, Sabina Mugusi, Omary Minzi, Eyasu Makonnen, Mohammed Janabi, Ferdinand Mugusi, Getachew Aderaye, Robert Hardwick, Beiyuan Fu, Maria Viskaduraki, Fengtang Yang, Edward J Hollox

Published in: BMC Infectious Diseases | Issue 1/2013

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Abstract

Background

The role of copy number variation of the CCL3L1 gene, encoding MIP1α, in contributing to the host variation in susceptibility and response to HIV infection is controversial. Here we analyse a sub-Saharan African cohort from Tanzania and Ethiopia, two countries with a high prevalence of HIV-1 and a high co-morbidity of HIV with tuberculosis.

Methods

We use a form of quantitative PCR called the paralogue ratio test to determine CCL3L1 gene copy number in 1134 individuals and validate our copy number typing using array comparative genomic hybridisation and fiber-FISH.

Results

We find no significant association of CCL3L1 gene copy number with HIV load in antiretroviral-naïve patients prior to initiation of combination highly active anti-retroviral therapy. However, we find a significant association of low CCL3L1 gene copy number with improved immune reconstitution following initiation of highly active anti-retroviral therapy (p = 0.012), replicating a previous study.

Conclusions

Our work supports a role for CCL3L1 copy number in immune reconstitution following antiretroviral therapy in HIV, and suggests that the MIP1α -CCR5 axis might be targeted to aid immune reconstitution.

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Metadata
Title
CCL3L1 copy number, HIV load, and immune reconstitution in sub-Saharan Africans
Authors
Eleni Aklillu
Linda Odenthal-Hesse
Jennifer Bowdrey
Abiy Habtewold
Eliford Ngaimisi
Getnet Yimer
Wondwossen Amogne
Sabina Mugusi
Omary Minzi
Eyasu Makonnen
Mohammed Janabi
Ferdinand Mugusi
Getachew Aderaye
Robert Hardwick
Beiyuan Fu
Maria Viskaduraki
Fengtang Yang
Edward J Hollox
Publication date
01-12-2013
Publisher
BioMed Central
Published in
BMC Infectious Diseases / Issue 1/2013
Electronic ISSN: 1471-2334
DOI
https://doi.org/10.1186/1471-2334-13-536

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