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BMC Infectious Diseases
Published in: BMC Infectious Diseases 1/2010

Open Access 01-12-2010 | Research article

In vivo expression of innate immunity markers in patients with mycobacterium tuberculosis infection

Authors: Pantelis Constantoulakis, Eftihia Filiou, Nikoletta Rovina, George Chras, Aggeliki Hamhougia, Simona Karabela, Adamandia Sotiriou, Charis Roussos, Nikolaos Poulakis

Published in: BMC Infectious Diseases | Issue 1/2010

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Abstract

Background

Toll-like receptors (TLRs), Coronin-1 and Sp110 are essential factors for the containment of Mycobacterium tuberculosis infection. The purpose of this study was to investigate the in vivo expression of these molecules at different stages of the infection and uncover possible relationships between these markers and the state of the disease.

Methods

Twenty-two patients with active tuberculosis, 15 close contacts of subjects with latent disease, 17 close contacts of subjects negative for mycobacterium antigens and 10 healthy, unrelated to patients, subjects were studied. Quantitative mRNA expression of Coronin-1, Sp110, TLRs-1,-2,-4 and -6 was analysed in total blood cells vs an endogenous house-keeping gene.

Results

The mRNA expression of Coronin-1, Sp110 and TLR-2 was significantly higher in patients with active tuberculosis and subjects with latent disease compared to the uninfected ones. Positive linear correlation for the expression of those factors was only found in the infected populations.

Conclusions

Our results suggest that the up-regulation of Coronin-1 and Sp110, through a pathway that also includes TLR-2 up-regulation may be involved in the process of tuberculous infection in humans. However, further studies are needed, in order to elucidate whether the selective upregulation of these factors in the infected patients could serve as a specific molecular marker of tuberculosis.
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Metadata
Title
In vivo expression of innate immunity markers in patients with mycobacterium tuberculosis infection
Authors
Pantelis Constantoulakis
Eftihia Filiou
Nikoletta Rovina
George Chras
Aggeliki Hamhougia
Simona Karabela
Adamandia Sotiriou
Charis Roussos
Nikolaos Poulakis
Publication date
01-12-2010
Publisher
BioMed Central
Published in
BMC Infectious Diseases / Issue 1/2010
Electronic ISSN: 1471-2334
DOI
https://doi.org/10.1186/1471-2334-10-243

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