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BMC Immunology
Published in: BMC Immunology 1/2013

Open Access 01-12-2013 | Research article

MicroRNA regulate immune pathways in T-cells in multiple sclerosis (MS)

Authors: Margareta Jernås, Clas Malmeström, Markus Axelsson, Intawat Nookaew, Hans Wadenvik, Jan Lycke, Bob Olsson

Published in: BMC Immunology | Issue 1/2013

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Abstract

Background

MicroRNA are small noncoding RNA molecules that are involved in the control of gene expression. To investigate the role of microRNA in multiple sclerosis (MS), we performed genome-wide expression analyses of mRNA and microRNA in T-cells from MS patients and controls.

Methods

Heparin-anticoagulated peripheral blood was collected from MS-patients and healthy controls followed by isolation of T-cells. MicroRNA and RNA from T-cells was prepared and hybridized to Affymetrix miR 2.0 array and Affymetrix U133Plus 2.0 Human Genome array (Santa Clara, CA), respectively. Verifications were performed with real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA).

Results

We identified 2,452 differentially expressed genes and 21 differentially expressed microRNA between MS patients and controls. By Kolmogorov-Smirnov test, 20 of 21 differentially expressed microRNA were shown to affect the expression of their target genes, many of which were involved in the immune system. Tumor necrosis factor ligand superfamily member 14 (TNFSF14) was a microRNA target gene significantly decreased in MS. The differential expression of mir-494, mir-197 and the predicted microRNA target gene TNFSF14 was verified by real-time PCR and ELISA.

Conclusion

These findings indicate that microRNA may be important regulatory molecules in T-cells in MS.
Appendix
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Metadata
Title
MicroRNA regulate immune pathways in T-cells in multiple sclerosis (MS)
Authors
Margareta Jernås
Clas Malmeström
Markus Axelsson
Intawat Nookaew
Hans Wadenvik
Jan Lycke
Bob Olsson
Publication date
01-12-2013
Publisher
BioMed Central
Published in
BMC Immunology / Issue 1/2013
Electronic ISSN: 1471-2172
DOI
https://doi.org/10.1186/1471-2172-14-32

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