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Open Access 01-12-2011 | Research article

Down-regulation of granulocyte-macrophage colony-stimulating factor by 3C-like proteinase in transfected A549 human lung carcinoma cells

Authors: Hsien-Hua Liao, Yao-Chen Wang, Miles Chih-Ming Chen, Hsien-Yu Tsai, Johnson Lin, Shui-Tein Chen, Gregory Jiazer Tsay, Sun-Long Cheng

Published in: BMC Immunology | Issue 1/2011

Abstract

Background

Severe Acute Respiratory Syndrome (SARS) is a severe respiratory illness caused by a novel virus, the SARS coronavirus (SARS-CoV). 3C-like protease (3CL^pro) of SARS-CoV plays a role in processing viral polypeptide precursors and is responsible of viral maturation. However, the function of 3CL^pro in host cells remains unknown. This study investigated how the 3CL^pro affected the secretion of cytokines in the gene-transfected cells.

Results

From immunofluorescence microscopy, the localization of c-myc tagged 3CL^pro was detected both in the cytoplasm and nucleus of transfected A549 cells. Expression of granulocyte-macrophage colony-stimulating factor (GM-CSF) was significantly decreased in 3CL^pro-transfected cells by both RT-PCR and ELISA, but without changes in other cytokines, i.e., IL-1β, IL-6, IL-8, IL12p40, TNF-α, and TGF-β. Furthermore, the protein levels of NF-kB decreased in 3CL^pro-transfected A549 cells when compared to EGFP transfected cells.

Conclusions

Our results suggest that the 3CL^pro may suppress expression of GM-CSF in transfected A549 cells through down-regulation of NF-kB production.

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Title: Down-regulation of granulocyte-macrophage colony-stimulating factor by 3C-like proteinase in transfected A549 human lung carcinoma cells
Authors: Hsien-Hua Liao
Yao-Chen Wang
Miles Chih-Ming Chen
Hsien-Yu Tsai
Johnson Lin
Shui-Tein Chen
Gregory Jiazer Tsay
Sun-Long Cheng
Publication date: 01-12-2011
Publisher: BioMed Central
Published in: BMC Immunology / Issue 1/2011
Electronic ISSN: 1471-2172
DOI: https://doi.org/10.1186/1471-2172-12-16

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Results

Conclusions

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