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Clinical and Translational Allergy
Published in: Clinical and Translational Allergy 1/2019

Open Access 01-12-2019 | Atopic Dermatitis | Review

Skin microdialysis: methods, applications and future opportunities—an EAACI position paper

Authors: Katrine Y. Baumann, Martin K. Church, Geraldine F. Clough, Sven Roy Quist, Martin Schmelz, Per Stahl Skov, Chris D. Anderson, Line Kring Tannert, Ana Maria Giménez-Arnau, Stefan Frischbutter, Jörg Scheffel, Marcus Maurer

Published in: Clinical and Translational Allergy | Issue 1/2019

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Abstract

Skin microdialysis (SMD) is a versatile sampling technique that can be used to recover soluble endogenous and exogenous molecules from the extracellular compartment of human skin. Due to its minimally invasive character, SMD can be applied in both clinical and preclinical settings. Despite being available since the 1990s, the technique has still not reached its full potential use as a tool to explore pathophysiological mechanisms of allergic and inflammatory reactions in the skin. Therefore, an EAACI Task Force on SMD was formed to disseminate knowledge about the technique and its many applications. This position paper from the task force provides an overview of the current use of SMD in the investigation of the pathogenesis of chronic inflammatory skin diseases, such as atopic dermatitis, chronic urticaria, psoriasis, and in studies of cutaneous events during type 1 hypersensitivity reactions. Furthermore, this paper covers drug hypersensitivity, UVB-induced- and neurogenic inflammation, and drug penetration investigated by SMD. The aim of this paper is to encourage the use of SMD and to make the technique easily accessible by providing an overview of methodology and applications, supported by standardized operating procedures for SMD in vivo and ex vivo.
Appendix
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Literature
1.
2.
Kreilgaard M. Assessment of cutaneous drug delivery using microdialysis. Adv Drug Deliv Rev. 2002;54(Suppl.):S99–121.PubMedCrossRef
3.
4.
Nielsen JB, Benfeldt E, Holmgaard R. Penetration through the skin barrier. In: Agner T, editor. Current problems in dermatology. Basel: Karger; 2016. p. 103–111.
5.
Boutsiouki P, Thompson JP, Clough GF. Effects of local blood flow on the percutaneous absorption of the organophosphorus compound malathion: a microdialysis study in man. Arch Toxicol. 2001;75:321–8.PubMedCrossRef
6.
Melgaard L, Hersini KJ, Gazerani P, Petersen LJ. Retrodialysis: a review of experimental and clinical applications of reverse microdialysis in the skin. Skin Pharmacol Physiol. 2013;26:160–74.PubMedCrossRef
7.
Clough GF, Jackson CL, Lee JJP, Jamal SC, Church MK. What can microdialysis tell us about the temporal and spatial generation of cytokines in allergen-induced responses in human skin in vivo? J Invest Dermatol. 2007;127:2799–806.PubMedCrossRef
8.
Sjögren F, Anderson C. Sterile trauma to normal human dermis invariably induces IL1beta, IL6 and IL8 in an innate response to ‘danger’. Acta Derm Venereol. 2009;89:459–65.PubMedCrossRef
9.
Church MK, Bewley AP, Clough GF, Burrows LJ, Ferdinand SI, Petersen LJ. Studies into the mechanisms of dermal inflammation using cutaneous microdialysis. Int Arch Allergy Immunol. 1997;113:131–3.PubMedCrossRef
10.
Church MK, Clough GF. Human skin mast cells: in vitro and in vivo studies. Ann Allergy Asthma Immunol. 1999;83:471–5.PubMedCrossRef
11.
Hersini KJ, Melgaard L, Gazerani P, Petersen LJ. Microdialysis of inflammatory mediators in the skin: a review. Acta Derm Venereol. 2014;94:501–11.PubMedCrossRef
12.
Petersen LJ, Church MK, Skov PS. Histamine is released in the wheal but not the flare following challenge of human skin in vivo: a microdialysis study. Clin Exp Allergy. 1997;27:284–95.PubMedCrossRef
13.
Schmelz M, Luz O, Averbeck B, Bickel A. Plasma extravasation and neuropeptide release in human skin as measured by intradermal microdialysis. Neurosci Lett. 1997;230:117–20.PubMedCrossRef
14.
Clough GF, Bennett AR, Church MK. Effects of H1 antagonists on the cutaneous vascular response to histamine and bradykinin: a study using scanning laser Doppler imaging. Br J Dermatol. 1998;138:806–14.PubMedCrossRef
15.
Christophe B, Maleux MR, Gillard M, Chatelain P, Peck MJ, Massingham R. The histamine H1-receptor antagonist cetirizine does not interact with bradykinin B1 or B2-receptors in vitro. Inflamm Res. 2004;53:S81–2.PubMedCrossRef
16.
Krause K, Giménez-Arnau A, Martinez-Escala E, Farré-Albadalejo M, Abajian M, Church MK, et al. Platelet-activating factor (PAF) induces wheal and flare skin reactions independent of mast cell degranulation. Allergy. 2013;68:256–8.PubMedCrossRef
17.
Rukwied R, Lischetzki G, McGlone F, Heyer G, Schmelz M. Mast cell mediators other than histamine induce pruritus in atopic dermatitis patients: a dermal microdialysis study. Br J Dermatol. 2000;142:1114–20.PubMedCrossRef
18.
Steinhoff M, Neisius U, Ikoma A, Fartasch M, Heyer G, Skov PS, et al. Proteinase-activated receptor-2 mediates itch: a novel pathway for pruritus in human skin. J Neurosci. 2003;23:6176–80.PubMedCrossRefPubMedCentral
19.
Leveque N, Robin S, Muret P, Mac-Mary S, Makki S, Humbert P. High iron and low ascorbic acid concentrations in the dermis of atopic dermatitis patients. Dermatology. 2003;207:261–4.PubMedCrossRef
20.
Quist SR, Wiswedel I, Doering I, Quist J, Gollnick HP. Effects of topical tacrolimus and polyunsaturated fatty acids on in vivo release of eicosanoids in atopic dermatitis during dermal microdialysis. Acta Derm Venereol. 2016;96:905–9.PubMedCrossRef
21.
Papoiu ADP, Wang H, Nattkemper L, Tey HL, Ishiuji Y, Chan YH, et al. A study of serum concentrations and dermal levels of NGF in atopic dermatitis and healthy subjects. Neuropeptides. 2011;45:417–22.PubMedPubMedCentralCrossRef
22.
Neisius U, Olsson R, Rukwied R, Lischetzki G, Schmelz M. Prostaglandin E2 induces vasodilation and pruritus, but no protein extravasation in atopic dermatitis and controls. J Am Acad Dermatol. 2002;47:28–32.PubMedCrossRef
23.
Ikoma A, Fartasch M, Heyer G, Miyachi Y, Handwerker H, Schmelz M. Painful stimuli evoke itch in patients with chronic pruritus: central sensitization for itch. Neurology. 2004;62:212–7.PubMedCrossRef
24.
Angst MS, Clark JD, Carvalho B, Tingle M, Schmelz M, Yeomans DC. Cytokine profile in human skin in response to experimental inflammation, noxious stimulation, and administration of a COX-inhibitor: a microdialysis study. Pain. 2008;139:15–27.PubMedCrossRef
25.
Angst MS, Tingle M, Schmelz M, Carvalho B, Yeomans DC. Human in vivo bioassay for the tissue-specific measurement of nociceptive and inflammatory mediators. J Vis Exp. 2008;22:1074.
26.
Sjögren F, Davidsson K, Sjöström M, Anderson CD. Cutaneous microdialysis: cytokine evidence for altered innate reactivity in the skin of psoriasis patients? AAPS J. 2012;14:187–95.PubMedPubMedCentralCrossRef
27.
Salgo R, Thaçi D, Boehncke S, Diehl S, Hofmann M, Boehncke WH. Microdialysis documents changes in the micromilieu of psoriatic plaques under continuous systemic therapy. Exp Dermatol. 2011;20:130–3.PubMedCrossRef
28.
Buerger C, Richter B, Woth K, Salgo R, Malisiewicz B, Diehl S, et al. Interleukin-1b interferes with epidermal homeostasis through induction of insulin resistance: implications for psoriasis pathogenesis. J Invest Dermatol. 2012;132:2206–14.PubMedCrossRef
29.
Quist SR, Quist J, Birkenmaier J, Stauch T, Gollnick HP. Pharmacokinetic profile of methotrexate in psoriatic skin via the oral or subcutaneous route using dermal microdialysis showing higher methotrexate bioavailability in psoriasis plaques than in non-lesional skin. J Eur Acad Dermatol Venereol. 2016;30:1537–43.PubMedCrossRef
30.
Krogstad AL, Lönnroth P, Larson G, Wallin BG. Increased interstitial histamine concentration in the psoriatic plaque. J Invest Dermatol. 1997;109:632–5.PubMedCrossRef
31.
Petersen LJ, Hansen U, Kristensen JK, Nielsen H, Skov PS, Nielsen HJ. Studies on mast cells and histamine release in psoriasis: the effect of ranitidine. Acta Derm Venereol. 1998;78:190–3.PubMedCrossRef
32.
Andersson T, Wårdell K, Anderson C. Human in vivo cutaneous microdialysis: estimation of histamine release in cold urticaria. Acta Derm Venereol. 1995;75:343–7.PubMed
33.
Nuutinen P, Harvima IT, Ackermann L. Histamine, but not leukotriene C4, is an essential mediator in cold urticaria wheals. Acta Derm Venereol. 2007;87:9–13.PubMedCrossRef
34.
Taskila K, Saarinen JV, Harvima IT, Harvima RJ. Histamine and LTC4 in stinging nettle-induced urticaria. Allergy. 2000;55:680–1.PubMedCrossRef
35.
Horsmanheimo L, Harvima IT, Harvima RJ, Brummer-Korvenkontio H, François G, Reunala T. Histamine and leukotriene C4 release in cutaneous mosquito-bite reactions. J Allergy Clin Immunol. 1996;98:408–11.PubMedCrossRef
36.
Tannert LK, Skov PS, Jensen LB, Maurer M, Bindslev-Jensen C. Cold urticaria patients exhibit normal skin levels of functional mast cells and histamine after tolerance induction. Dermatology. 2012;224:101–5.CrossRef
37.
Krause K, Spohr A, Zuberbier T, Church MK, Maurer M. Up-dosing with bilastine results in improved effectiveness in cold contact urticaria. Allergy. 2013;68:921–8.PubMedCrossRef
38.
Tannert LK, Falkencrone S, Mortz CG, Bindslev-Jensen C, Skov PS. Is a positive intracutaneous test induced by penicillin mediated by histamine? A cutaneous microdialysis study in penicillin-allergic patients. Clin Transl Allergy. 2017;7:1–9.CrossRef
39.
Averbeck M, Beilharz S, Bauer M, Gebhardt C, Hartmann A, Hochleitner K, et al. In situ profiling and quantification of cytokines released during ultraviolet B-induced inflammation by combining dermal microdialysis and protein microarrays. Exp Dermatol. 2006;15:447–54.PubMedCrossRef
40.
Grundmann J-U, Wiswedel I, Hirsch D, Gollnick HPM. Detection of monohydroxyeicosatetraenoic acids and F2-isoprostanes in microdialysis samples of human UV-irradiated skin by gas chromatography-mass spectrometry. Skin Pharmacol Physiol. 2004;17:37–41.PubMedCrossRef
41.
Quist SR, Wiswedel I, Quist J, Gollnick HP. Kinetic profile of inflammation markers in human skin in vivo following exposure to ultraviolet B indicates synchronic release of cytokines and prostanoids. Acta Derm Venereol. 2016;96:910–6.PubMedCrossRef
42.
Wiswedel I, Grundmann JU, Boschmann M, Krautheim A, Böckelmann R, Peter DS, et al. Effects of UVB irradiation and diclofenac on F2-isoprostane/prostaglandin concentrations in keratinocytes and microdialysates of human skin. J Invest Dermatol. 2007;127:1794–7.PubMedCrossRef
43.
Klede M, Clough G, Lischetzki G, Schmelz M. The effect of the nitric oxide synthase inhibitor N-nitro-l-arginine-methyl ester on neuropeptide-induced vasodilation and protein extravasation in human skin. J Vasc Res. 2003;40:105–14.PubMedCrossRef
44.
Huttunen M, Harvima IT, Ackermann L, Harvima RJ, Naukkarinen A, Horsmanheimo M. Neuropeptide- and capsaicin-induced histamine release in skin monitored with the microdialysis technique. Acta Derm Venereol. 1996;76:205–9.PubMed
45.
Petersen LJ, Winge K, Brodin E, Skov PS. No release of histamine and substance P in capsaicin-induced neurogenic inflammation in intact human skin in vivo: a microdialysis study. Clin Exp Allergy. 1997;27:957–65.PubMedCrossRef
46.
Sauerstein K, Klede M, Hilliges M, Schmelz M. Electrically evoked neuropeptide release and neurogenic inflammation differ between rat and human skin. J Physiol. 2000;529:803–10.PubMedPubMedCentralCrossRef
47.
Weidner C, Klede M, Rukwied R, Lischetzki G, Neisius U, Skov PS, et al. Acute effects of substance P and calcitonin gene-related peptide in human skin—a microdialysis study. J Invest Dermatol. 2000;115:1015–20.PubMedCrossRef
48.
Weber M, Birklein F, Neundörfer B, Schmelz M. Facilitated neurogenic inflammation in complex regional pain syndrome. Pain. 2001;91:251–7.PubMedCrossRef
49.
Leis S, Weber M, Isselmann A, Schmelz M, Birklein F. Substance-P-induced protein extravasation is bilaterally increased in complex regional pain syndrome. Exp Neurol. 2003;183:197–204.PubMedCrossRef
50.
Leis S, Weber M, Schmelz M, Birklein F. Facilitated neurogenic inflammation in unaffected limbs of patients with complex regional pain syndrome. Neurosci Lett. 2004;359:163–6.PubMedCrossRef
51.
Schlereth T, Breimhorst M, Werner N, Pottschmidt K, Drummond PD, Birklein F. Inhibition of neuropeptide degradation suppresses sweating but increases the area of the axon reflex flare. Exp Dermatol. 2013;22:299–301.PubMedCrossRef
52.
Anderson C, Andersson T, Molander M. Ethanol absorption across human skin measured by in vivo microdialysis technique. Acta Derm Venereol. 1991;71:389–93.PubMed
53.
Müller M, Schmid R, Wagner O, Osten B, Shayganfar H, Eichler HG. In vivo characterization of transdermal drug transport by microdialysis. J Control Release. 1995;37:49–57.CrossRef
54.
Cross SE, Anderson C, Roberts MS. Topical penetration of commercial salicylate esters and salts using human isolated skin and clinical microdialysis studies. Br J Clin Pharmacol. 1998;46:29–35.PubMedPubMedCentralCrossRef
55.
Kreilgaard M, Kemme MJB, Burggraaf J, Schoemaker RC, Cohen AF. Influence of a microemulsion vehicle on cutaneous bioequivalence of a lipophilic model drug assessed by microdialysis and pharmacodynamics. Pharm Res. 2001;18:593–9.PubMedCrossRef
56.
Benfeldt E, Hansen SH, Vølund A, Menné T, Shah VP. Bioequivalence of topical formulations in humans: evaluation by dermal microdialysis sampling and the dermatopharmacokinetic method. J Invest Dermatol. 2007;127:170–8.PubMedCrossRef
57.
Herkenne C, Alberti I, Naik A, Kalia YN, Mathy FX, Préat V, et al. In vivo methods for the assessment of topical drug bioavailability. Pharm Res. 2008;25:87–103.PubMedCrossRef
58.
Holmgaard R, Nielsen JB, Benfeldt E. Microdialysis sampling for investigations of bioavailability and bioequivalence of topically administered drugs: current state and future perspectives. Skin Pharmacol Physiol. 2010;23:225–43.PubMedCrossRef
59.
Au WL, Skinner MF, Benfeldt E, Verbeeck RK, Kanfer I. Application of dermal microdialysis for the determination of bioavailability of clobetasol propionate applied to the skin of human subjects. Skin Pharmacol Physiol. 2012;25:17–24.PubMedCrossRef
60.
Bodenlenz M, Aigner B, Dragatin C, Liebenberger L, Zahiragic S, Höfferer C, et al. Clinical applicability of dOFM devices for dermal sampling. Ski Res Technol. 2013;19:474–83.
61.
Bodenlenz M, Tiffner KI, Raml R, Augustin T, Dragatin C, Birngruber T, et al. open flow microperfusion as a dermal pharmacokinetic approach to evaluate topical bioequivalence. Clin Pharmacokinet. 2017;56:91–8.PubMedCrossRef
62.
Müller M, editor. Microdialysis in drug development. 1st ed. Berlin: Springer; 2013.
63.
Gill C, Parkinson E, Church MK, Skipp P, Scott D, White AJ, et al. A qualitative and quantitative proteomic study of human microdialysate and the cutaneous response to injury. AAPS J. 2011;13:309–17.PubMedPubMedCentralCrossRef
64.
Lischetzki G, Rukwied R, Handwerker HO, Schmelz M. Nociceptor activation and protein extravasation induced by inflammatory mediators in human skin. Eur J Pain. 2001;5:49–57.PubMedCrossRef
65.
Benfeldt E, Serup J, Menné T. Effect of barrier perturbation on cutaneous salicylic acid penetration in human skin: in vivo pharmacokinetics using microdialysis and non-invasive quantification of barrier function. Br J Dermatol. 1999;140:739–48.PubMedCrossRef
66.
Smith CJ, Kenney WL, Alexander LM. Regional relation between skin blood flow and sweating to passive heating and local administration of acetylcholine in young, healthy humans. Am J Physiol Regul Integr Comp Physiol. 2013;304:R566–73.PubMedPubMedCentralCrossRef
67.
Rukwied R, Zeck S, Schmelz M, McGlone F. Sensitivity of human scalp skin to pruritic stimuli investigated by intradermal microdialysis in vivo. J Am Acad Dermatol. 2002;47:245–50.PubMedCrossRef
68.
Setälä L, Joukainen S, Uusaro A, Alhava E, Härmä M. Metabolic response in microvascular flaps during partial pedicle obstruction and hypovolemic shock. J Reconstr Microsurg. 2007;23:489–96.PubMedCrossRef
69.
Anderson C, Andersson T, Wårdell K. Changes in skin circulation after insertion of a microdialysis probe visualized by laser Doppler perfusion imaging. J Invest Dermatol. 1994;102:807–11.PubMedCrossRef
70.
Clough GF, Boutsiouki P, Church MK, Michel CC. Effects of blood flow on the in vivo recovery of a small diffusible molecule by microdialysis in human skin. J Pharmacol Exp Ther. 2002;302:681–6.PubMedCrossRef
71.
Petersen LJ, Poulsen LK, Søndergaard J, Skov PS. The use of cutaneous microdialysis to measure substance P-induced histamine release in intact human skin in vivo. J Allergy Clin Immunol. 1994;94:773–83.PubMedCrossRef
72.
Rogers ML, Feuerstein D, Leong CL, Takagaki M, Niu X, Graf R, et al. Continuous online microdialysis using microfluidic sensors: dynamic neurometabolic changes during spreading depolarization. ACS Chem Neurosci. 2013;4:799–807.PubMedPubMedCentralCrossRef
73.
Saylor RA, Reid EA, Lunte SM. Microchip electrophoresis with electrochemical detection for the determination of analytes in the dopamine metabolic pathway. Electrophoresis. 2015;36:1912–9.PubMedPubMedCentralCrossRef
74.
Petersen LJ, Brasso K, Pryds M, Skov PS. Histamine release in intact human skin by monocyte chemoattractant factor-1, RANTES, macrophage inflammatory protein-1α, stem cell factor, anti-IgE, and codeine as determined by an ex vivo skin microdialysis technique. J Allergy Clin Immunol. 1996;98:790–6.PubMedCrossRef
75.
Leveque N, Robin S, Makki S, Muret P, Rougier A, Humbert P. Iron and ascorbic acid concentrations in human dermis with regard to age and body sites. Gerontology. 2003;49:117–22.PubMedCrossRef
76.
Leveque N, Muret P, Makki S, Mac-Mary S, Kantelip JP, Humbert P. Ex vivo cutaneous absorption assessment of a stabilized ascorbic acid formulation using a microdialysis system. Skin Pharmacol Physiol. 2004;17:298–303.PubMedCrossRef
77.
Holmgaard R, Benfeldt E, Bangsgaard N, Sorensen JA, Brosen K, Nielsen F, et al. Probe depth matters in dermal microdialysis sampling of benzoic acid after topical application: an ex vivo study in human skin. Skin Pharmacol Physiol. 2012;25:9–16.PubMedCrossRef
78.
Döge N, Hönzke S, Schumacher F, Balzus B, Colombo M, Hadam S, et al. Ethyl cellulose nanocarriers and nanocrystals differentially deliver dexamethasone into intact, tape-stripped or sodium lauryl sulfate-exposed ex vivo human skin—assessment by intradermal microdialysis and extraction from the different skin layers. J Control Release. 2016;242:25–34.PubMedCrossRef
79.
Holmgaard R, Benfeldt E, Nielsen JB, Gatschelhofer C, Sorensen J, Höfferer C, et al. Comparison of open-flow microperfusion and microdialysis methodologies when sampling topically applied fentanyl and benzoic acid in human dermis ex vivo. Pharm Res. 2012;29:1808–20.PubMedCrossRef
80.
Boelsma E, Anderson C, Karlsson AMJ, Ponec M. Microdialysis technique as a method to study the percutaneous penetration of methyl nicotinate through excised human skin, reconstructed epidermis, and human skin in vivo. Pharm Res. 2000;17:141–7.PubMedCrossRef
81.
Schumacher S, Stahl J, Bäumer W, Seitz JM, Bach FW, Petersen LJ, et al. Ex vivo examination of the biocompatibility of biodegradable magnesium via microdialysis in the isolated perfused bovine udder model. Int J Artif Organs. 2011;34:34–43.PubMedCrossRef
82.
Chaurasia CS, Müller M, Bashaw ED, Benfeldt E, Bolinder J, Bullock R, et al. AAPS-FDA workshop white paper: microdialysis principles, application and regulatory perspectives. Pharm Res. 2007;24:1014–25.PubMedCrossRef
83.
World Medical Association. World Medical Association Declaration of Helsinki: ethical principles for medical research involving human subjects. JAMA. 2013;310:2191–4.CrossRef
84.
Egawa G, Honda T, Tanizaki H, Doi H, Miyachi Y, Kabashima K. In vivo imaging of t-cell motility in the elicitation phase of contact hypersensitivity using two-photon microscopy. J Invest Dermatol. 2011;131:977–9.PubMedCrossRef
85.
Talló K, Moner V, De Cabo M, Cócera M, López O. Vesicular nanostructures composed of oleic acid and phosphatidylcholine: effect of pH and molar ratio. Chem Phys Lipids. 2018;213:96–101.PubMedCrossRef
86.
Wang X, Stenken JA. Microdialysis sampling membrane performance during in vitro macromolecule collection. Anal Chem. 2006;78:6026–34.PubMedCrossRef
87.
88.
89.
Shukla C, Bashaw ED, Stagni G, Benfeldt E. Applications of dermal microdialysis: a review. J Drug Deliv Sci Technol. 2014;24:259–69.CrossRef
90.
Ward KW, Medina SJ, Portelli ST, Mahar Doan KM, Spengler MD, Ben MM, et al. Enhancement of in vitro and in vivo microdialysis recovery of SB-265123 using Intralipid® and Encapsin® as perfusates. Biopharm Drug Dispos. 2003;24:17–25.PubMedCrossRef
91.
Helmy A, Carpenter KLH, Skepper JN, Kirkpatrick PJ, Pickard JD, Hutchinson PJ. Microdialysis of cytokines: methodological considerations, scanning electron microscopy, and determination of relative recovery. J Neurotrauma. 2009;26:549–61.PubMedCrossRef
92.
Keeler GD, Durdik JM, Stenken JA. Comparison of microdialysis sampling perfusion fluid components on the foreign body reaction in rat subcutaneous tissue. Eur J Pharm Sci. 2014;57:60–7.PubMedCrossRef
93.
Trickler WJ, Miller DW. Use of osmotic agents in microdialysis studies to improve the recovery of macromolecules. J Pharm Sci. 2003;92:1419–27.PubMedCrossRef
94.
Plock N, Kloft C. Microdialysis—theoretical background and recent implementation in applied life-sciences. Eur J Pharm Sci. 2005;25:1–24.PubMedCrossRef
95.
96.
Chu J, Koudriavtsev V, Hjort K, Dahlin AP. Fluorescence imaging of macromolecule transport in high molecular weight cut-off microdialysis. Anal Bioanal Chem. 2014;406:7601–9.PubMedCrossRef
97.
Benfeldt E. Application of microdialysis in assessing cutaneous bioavailability. In: Shah VP, Maibach HI, Jenner J, editors. Topical drug bioavailability, bioequivalence, and penetration. Berlin: Springer; 2014. p. 153–65.
98.
de Lange ECM. Recovery and calibration techniques: toward quantitative microdialysis. In: Müller M, editor. Microdialysis in drug development. Berlin: Springer; 2013. p. 13–34.CrossRef
99.
Lindberger M, Tomson T, Ståhle L. Microdialysis sampling of carbamazepine, phenytoin and phenobarbital in subcutaneous extracellular fluid and subdural cerebrospinal fluid in humans: an in vitro and in vivo study of adsorption to the sampling device. Pharmacol Toxicol. 2002;91:158–65.PubMedCrossRef
100.
Quist SR, Kirbs C, Kloft C, Gollnick HP. Cytokine and chemokine recovery is increased by colloid perfusates during dermal microdialysis. Materials (Basel). 2018;11:1–23.CrossRef
101.
Jadhav SB, Khaowroongrueng V, Derendorf H. Microdialysis of large molecules. J Pharm Sci. 2016;105:3233–42.PubMedCrossRef
102.
Khan F, Pharo A, Lindstad JK, Mollnes TE, Tønnessen TI, Pischke SE. Effect of perfusion fluids on recovery of inflammatory mediators in microdialysis. Scand J Immunol. 2015;82:467–75.PubMedCrossRef
103.
Kirbs C, Kloft C. In vitro microdialysis recovery and delivery investigation of cytokines as prerequisite for potential biomarker profiling. Eur J Pharm Sci. 2014;57:48–59.PubMedCrossRef
104.
Chaurasia CS. In vivo microdialysis sampling: theory and applications. Biomed Chromatogr. 1999;13:317–32.PubMedCrossRef
105.
Groth L, Serup J. Cutaneous microdialysis in man: effects of needle insertion trauma and anaesthesia on skin perfusion, erythema and skin thickness. Acta Derm Venereol. 1998;78:5–9.PubMedCrossRef
106.
Stenken J, Church MK, Gill C, Clough GF. How minimally invasive is microdialysis sampling? A cautionary note for cytokine collection in human skin and other clinical studies. AAPS J. 2010;12:73–8.PubMedCrossRef
107.
Weber A, Knop J, Maurer M. Pattern analysis of human cutaneous mast cell populations by total body surface mapping. Br J Dermatol. 2003;148:224–8.PubMedCrossRef
108.
109.
Cracowski JL, Lorenzo S, Minson CT. Effects of local anaesthesia on subdermal needle insertion pain and subsequent tests of microvascular function in human. Eur J Pharmacol. 2007;559:150–4.PubMedCrossRef
110.
Tettey-Amlalo RNO, Kanfer I, Skinner MF, Benfeldt E, Verbeeck RK. Application of dermal microdialysis for the evaluation of bioequivalence of a ketoprofen topical gel. Eur J Pharm Sci. 2009;36:219–25.PubMedCrossRef
Metadata
Title
Skin microdialysis: methods, applications and future opportunities—an EAACI position paper
Authors
Katrine Y. Baumann
Martin K. Church
Geraldine F. Clough
Sven Roy Quist
Martin Schmelz
Per Stahl Skov
Chris D. Anderson
Line Kring Tannert
Ana Maria Giménez-Arnau
Stefan Frischbutter
Jörg Scheffel
Marcus Maurer
Publication date
01-12-2019
Publisher
BioMed Central
Published in
Clinical and Translational Allergy / Issue 1/2019
Electronic ISSN: 2045-7022
DOI
https://doi.org/10.1186/s13601-019-0262-y

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