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Journal of Experimental & Clinical Cancer Research

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Open Access 01-12-2016 | Research

Long noncoding RNA NEAT1 promotes laryngeal squamous cell cancer through regulating miR-107/CDK6 pathway

Authors: Peng Wang, Tianyi Wu, Han Zhou, Qianqian Jin, Guoqing He, Haoyang Yu, Lijia Xuan, Xin Wang, Linli Tian, Yanan Sun, Ming Liu, Lingmei Qu

Published in: Journal of Experimental & Clinical Cancer Research | Issue 1/2016

Abstract

Background

Long noncoding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) plays key role in the progression of some human cancers. However, the role of NEAT1 in human laryngeal squamous cell cancer (LSCC) is still unknown. We therefore investigated the expression and function of NEAT1 in LSCC.

Methods

NEAT1 level in LSCC and adjacent non-neoplastic tissues were detected by qRT-PCR. NEAT1 was knockdown in LSCC cells and cell proliferation, apoptosis and cell cycle were examined. The growth of xenografts with NEAT1 knockdown LSCC cells was analyzed.

Results

NEAT1 level was significantly higher in LSCC than in corresponding adjacent non-neoplastic tissues, and patients with neck nodal metastasis or advanced clinical stage had higher NEAT1 expression. Moreover, siRNA mediated NEAT1 knockdown significantly inhibited the proliferation and induced apoptosis and cell cycle arrest at G1 phase in LSCC cells. The growth of LSCC xenografts was significantly suppressed by the injection of NEAT1 siRNA lentivirus. Furthermore, NEAT1 regulated CDK6 expression in LSCC cells which was mediated by miR-107.

Conclusion

NEAT1 plays an oncogenic role in the tumorigenesis of LSCC and may serve as a potential target for therapeutic intervention.

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Title: Long noncoding RNA NEAT1 promotes laryngeal squamous cell cancer through regulating miR-107/CDK6 pathway
Authors: Peng Wang
Tianyi Wu
Han Zhou
Qianqian Jin
Guoqing He
Haoyang Yu
Lijia Xuan
Xin Wang
Linli Tian
Yanan Sun
Ming Liu
Lingmei Qu
Publication date: 01-12-2016
Publisher: BioMed Central
Published in: Journal of Experimental & Clinical Cancer Research / Issue 1/2016
Electronic ISSN: 1756-9966
DOI: https://doi.org/10.1186/s13046-016-0297-z

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