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Diagnostic Pathology

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Open Access 01-12-2014 | Research

RETRACTED ARTICLE: Dysregulation of cell cycle related genes and microRNAs distinguish the low- from high-risk of prostate cancer

Authors: Jiling Wen, Rongbing Li, Xiaofei Wen, Guangming Chou, Jiasun Lu, Xuelei Wang, Yongchao Jin

Published in: Diagnostic Pathology | Issue 1/2014

Abstract

Background

Prostate cancer (PCa) is a biologically heterogeneous disease with considerable variation in clinical aggressiveness. In this study, bioinformatics was used to detect the patterns of gene expression alterations of PCa patients.

Methods

The gene expression profile GSE21034 and GSE21036 were downloaded from Gene Expression Omnibus (GEO) database. Significantly changed mRNA transcripts and microRNAs were identified between subtypes with favorable (cluster 2) and unfavorable (cluster 5) prognosis by two-side unequal variances t test. MicroRNAs and their potential target genes were identified by TargetScan and miRTarBase, respectively. Besides, the overlapped genes between the target genes of microRNAs and mRNA transcripts were assessed by Fisher’ exact test (one side). The functional annotation was performed by DAVID, followed by construction of protein-protein interaction (PPI) network.

Results

Compared to cluster 2, 1556 up-regulated and 1288 down-regulated transcripts were identified in cluster 5. Total 28 microRNAs were up-regulated and 30 microRNAs were down-regulated in cluster 5. Besides, 12 microRNAs target transcripts were significantly overlapped with down-regulated transcripts in cluster 5 with none of them was found overlapped with up-regulated transcripts. Functional annotation showed that cell cycle was the most significant function. In the PPI network, BRCA1, CDK1, TK1 and TRAF2 were hub protein of signature genes in cluster 5, and TGFBR1, SMAD2 and SMAD4 were hub proteins of signature gnens in cluster 2.

Conclusions

Our findings raise the possibility that genes related with cell cycle and dysregulated miRNA at diagnosis might have clinical utility in distinguishing low- from high-risk PCa patients.

Virtual slides

The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/13000_2014_156

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Title: RETRACTED ARTICLE: Dysregulation of cell cycle related genes and microRNAs distinguish the low- from high-risk of prostate cancer
Authors: Jiling Wen
Rongbing Li
Xiaofei Wen
Guangming Chou
Jiasun Lu
Xuelei Wang
Yongchao Jin
Publication date: 01-12-2014
Publisher: BioMed Central
Published in: Diagnostic Pathology / Issue 1/2014
Electronic ISSN: 1746-1596
DOI: https://doi.org/10.1186/s13000-014-0156-1

Keynote webinar | Spotlight on medication adherence

Springer Medicine

RETRACTED ARTICLE: Dysregulation of cell cycle related genes and microRNAs distinguish the low- from high-risk of prostate cancer

Abstract

Background

Methods

Results

Conclusions

Virtual slides

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

Virtual slides

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