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Fluids and Barriers of the CNS
Published in: Fluids and Barriers of the CNS 1/2020

01-12-2020 | Magnetic Resonance Imaging | Research

Effects of aging on hydrocephalus after intraventricular hemorrhage

Authors: Yingfeng Wan, Feng Gao, Fenghui Ye, Weiming Yang, Ya Hua, Richard F. Keep, Guohua Xi

Published in: Fluids and Barriers of the CNS | Issue 1/2020

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Abstract

Background

Hydrocephalus is a common and major complication that affects outcome after intraventricular hemorrhage (IVH). While aging impacts the occurrence of hydrocephalus in patients with IVH this and the underlying mechanisms have received little attention. The present investigation, therefore, studied the impact of aging on hydrocephalus after IVH in a rat model.

Methods

Young and aged (3 and 18 months old, respectively) male Fischer 344 rats had an intraventricular injection of 200 μl autologous blood or saline. Ventricular volume was estimated using magnetic resonance imaging (MRI), while ventricular wall damage, heme oxygenase-1 (HO-1) and epiplexus cell activation were quantified by histological staining and Western blot. Additionally, the impact of intraventricular iron injection was examined in young and aged rats.

Results

Intraventricular injection of autologous blood induced hydrocephalus in both young and aged rats but ventricular volumes were larger in aged rats compared to young rats from day 3 to day 14 followed IVH. In addition, ventricular wall damage and periventricular HO-1 upregulation were greater in aged versus young rats on day 1 after IVH. Aged rats also had more choroid plexus epiplexus cells on day 14 after IVH. Additionally, organized hematomas were observed in 23% (3/13) of aged rats but not in young rats after IVH. Organized hematomas in aged rats showed larger T2* lesions on MRI compared to rats with non-organized hematomas. Similar to the effects of IVH, intraventricular injection of iron resulted in more epiplexus cells activation and more severe hydrocephalus in aged compared to young rats.

Conclusions

IVH causes more severe hydrocephalus in aged compared to young rats. Enhanced ventricular wall damage, epiplexus cell activation and iron overload may contribute to this aggravated hydrocephalus development in aged animals.
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Metadata
Title
Effects of aging on hydrocephalus after intraventricular hemorrhage
Authors
Yingfeng Wan
Feng Gao
Fenghui Ye
Weiming Yang
Ya Hua
Richard F. Keep
Guohua Xi
Publication date
01-12-2020
Publisher
BioMed Central
Published in
Fluids and Barriers of the CNS / Issue 1/2020
Electronic ISSN: 2045-8118
DOI
https://doi.org/10.1186/s12987-020-0169-y

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