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Virology Journal
Published in: Virology Journal 1/2016

Open Access 01-12-2016 | Research

HPV genotype distribution in Brazilian women with and without cervical lesions: correlation to cytological data

Authors: Toni Ricardo Martins, Cristina Mendes de Oliveira, Luciana Reis Rosa, Cristiane de Campos Centrone, Célia Luiza Regina Rodrigues, Luisa Lina Villa, José Eduardo Levi

Published in: Virology Journal | Issue 1/2016

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Abstract

Background

Human Papillomavirus (HPV) genotype distribution varies according to the method of assessment and population groups. This study analyzed type-specific HPV infections among women ranging from 14–95 years old, displaying normal and abnormal cytology, from São Paulo and Barretos cities, Brazil.

Methods

Women found positive for High Risk-HPVs DNA by either the Hybrid Capture 2 (HC2) or Cobas HPV Test (n = 431) plus a random sample of 223 negative by both assays and 11 samples with indeterminate results, totalizing 665 samples, were submitted to HPV detection by the PapilloCheck test. Cytological distribution included 499 women with a cytological result of Negative for Intraepithelial Lesion or Malignancy and 166 with some abnormality as follows: 54 Atypical Squamous Cells of Undetermined Significance; 66 Low-Grade Squamous Intraepithelial Lesion; 43 High-Grade Squamous Intraepithelial Lesion and 3 (0.5 %) Invasive Cervical Cancer.

Results

From the 323 samples (48.6 %) that had detectable HPV-DNA by the PapilloCheck assay, 31 were HPV negative by the cobas HPV and HC2 assays. Out of these 31 samples, 14 were associated with HR-HPVs types while the remaining 17 harbored exclusively low-risk HPVs. In contrast, 49 samples positive by cobas HPV and HC 2 methods tested negative by the PapilloCheck assay (19.8 %). Overall, the most frequent HR-HPV type was HPV 16 (23.2 %), followed by 56 (21.0 %), 52 (8.7 %) and 31 (7.7 %) and the most frequent LR-HPV type was HPV 42 (12.1 %) followed by 6 (6.2 %). Among the HR-HPV types, HPV 56 and 16 were the most frequent types in NILM, found in 19.1 and 17.7 % of the patients respectively while in HSIL and ICC cases, HPV 16 was the predominant type, detected in 37.2 and 66.7 % of these samples.

Conclusions

In the population studied, HPV 16 and 56 were the most frequently detected HR-HPV types. HPV 56 was found mainly in LSIL and NILM suggesting a low oncogenic potential. HPV 16 continues to be the most prevalent type in high-grade lesions whereas HPV 18 was found in a low frequency both in NILM and abnormal smears. Surveillance of HPV infections by molecular methods is an important tool for the development and improvement of prevention strategies.
Literature
1.
Kocjan BJ, Bzhalava D, Forslund O, Dillner J, Poljak M. Molecular methods for identification and characterization of novel papillomaviruses. Clin Microbiol Infect. 2015;21(9):808–16.CrossRefPubMed
2.
Munoz N, Bosch FX, de Sanjose S, Herrero R, Castellsague X, Shah KV, et al. Epidemiologic classification of human papillomavirus types associated with cervical cancer. N Engl J Med. 2003;348(6):518–27.CrossRef
3.
Piana A, Sotgiu G, Castiglia P, Pischedda S, Cocuzza C, Capobianco G, et al. Prevalence and type distribution of human papillomavirus infection in women from North Sardinia, Italy. BMC Public Health. 2011;11:785.CrossRefPubMedPubMedCentral
4.
5.
Bouvard V, Baan R, Straif K, Grosse Y, Secretan B, El Ghissassi F, et al. A review of human carcinogens--Part B: biological agents. Lancet Oncol. 2009;10(4):321–2.CrossRefPubMed
6.
de Sanjose S, Quint WG, Alemany L, Geraets DT, Klaustermeier JE, Lloveras B, et al. Human papillomavirus genotype attribution in invasive cervical cancer: a retrospective cross-sectional worldwide study. Lancet Oncol. 2010;11(11):1048–56.CrossRefPubMed
7.
de Sanjose S, Diaz M, Castellsague X, Clifford G, Bruni L, Munoz N, et al. Worldwide prevalence and genotype distribution of cervical human papillomavirus DNA in women with normal cytology: a meta-analysis. Lancet Infect Dis. 2007;7(7):453–9.CrossRefPubMed
8.
Fernandes JV, Meissner Rde V, de Carvalho MG, Fernandes TA, de Azevedo PR, Villa LL. Prevalence of HPV infection by cervical cytologic status in Brazil. Int J Gynaecol Obstet. 2009;105(1):21–4.CrossRefPubMed
9.
Matsumoto K, Oki A, Furuta R, Maeda H, Yasugi T, Takatsuka N, et al. Predicting the progression of cervical precursor lesions by human papillomavirus genotyping: a prospective cohort study. Int J Cancer. 2011;128(12):2898–910.CrossRefPubMed
10.
Powell NG, Hibbitts SJ, Boyde AM, Newcombe RG, Tristram AJ, Fiander AN. The risk of cervical cancer associated with specific types of human papillomavirus: a case–control study in a UK population. Int J Cancer. 2011;128(7):1676–82.CrossRefPubMed
11.
WHO. The World Health Organization - Information Centre on HPV and Cervical Cancer (HPV Information Centre). Human Papillomavirus and Related Diseases. 2014. [cited 2014 Out 28] Avaiable at http://​www.​who.​int/​hpvcentre. 2014.
12.
13.
Otero-Motta AP, Ordonez JL, Gonzalez-Celador R, Rivas B, Macias Mdel C, Bullon A, et al. Prevalence of human papillomavirus genotypes in cytologic abnormalities from unvaccinated women living in north-western Spain. APMIS. 2011;119(3):204–15.CrossRefPubMed
14.
Levi JE, Longatto-filho A, Eluf-neto J, Rodrigues CL, Oliveira CM, Carloni AC, Lorenzi AT, Tacla M, Fregnani JHTG, Ab’saber AM, Scapulatempo C, Villa LL. Evaluation of HPV molecular tests in primary screening for cervical cancer in Brazil. Open J Obstet Gynecol. 2014;4:470–8.CrossRef
15.
Bruno A, Serravalle K, Travassos AG, Lima BGC. Distribuição dos Genótipos de papillomavírus humano em mulheres do estado da Bahia, Brasil. Rev Bras Ginecol Obstet. 2014;36(9):416–22.CrossRefPubMed
16.
Herrero R, Castle PE, Schiffman M, Bratti MC, Hildesheim A, Morales J, et al. Epidemiologic profile of type-specific human papillomavirus infection and cervical neoplasia in Guanacaste, Costa Rica. J Infect Dis. 2005;191(11):1796–807.CrossRefPubMed
17.
Kjaer SK, Breugelmans G, Munk C, Junge J, Watson M, Iftner T. Population-based prevalence, type- and age-specific distribution of HPV in women before introduction of an HPV-vaccination program in Denmark. Int J Cancer. 2008;123(8):1864–70.CrossRefPubMed
18.
Li N, Franceschi S, Howell-Jones R, Snijders PJ, Clifford GM. Human papillomavirus type distribution in 30,848 invasive cervical cancers worldwide: Variation by geographical region, histological type and year of publication. Int J Cancer. 2011;128(4):927–35.CrossRefPubMed
19.
Bruni L, Diaz M, Castellsague X, Ferrer E, Bosch FX, de Sanjose S. Cervical human papillomavirus prevalence in 5 continents: meta-analysis of 1 million women with normal cytological findings. J Infect Dis. 2010;202(12):1789–99.CrossRefPubMed
20.
Noronha V, Mello W, Villa L, Brito A, Macedo R, Bisi F, et al. Human papillomavirus associated with uterine cervix lesions. Rev Soc Bras Med Trop. 1999;32(3):235–40.CrossRefPubMed
21.
Eluf-Neto J, Booth M, Munoz N, Bosch FX, Meijer CJ, Walboomers JM. Human papillomavirus and invasive cervical cancer in Brazil. Br J Cancer. 1994;69(1):114–9.CrossRefPubMedPubMedCentral
22.
Oliveira-Silva M, Lordello CX, Zardo LM, Bonvicino CR, Moreira MA. Human Papillomavirus in Brazilian women with and without cervical lesions. Virol J. 2011;8:4.CrossRefPubMedPubMedCentral
23.
Baldez da Silva MF, Chagas BS, Guimaraes V, Katz LM, Felix PM, Miranda PM, et al. HPV31 and HPV33 incidence in cervical samples from women in Recife, Brazil. Genet Mol Res. 2009;8(4):1437–43.CrossRefPubMed
24.
Rabelo-Santos SH, Zeferino L, Villa LL, Sobrinho JP, Amaral RG, Magalhaes AV. Human papillomavirus prevalence among women with cervical intraepithelial neoplasia III and invasive cervical cancer from Goiania, Brazil. Mem Inst Oswaldo Cruz. 2003;98(2):181–4.CrossRefPubMed
25.
Tozetti IA, Scapulatempo IDL, Kawski VL, Ferreira AW, Levi JE. Multiple types of human papillomavirus in cervical samples in women in Campo Grande, MS, Brazil. BJID. 2006;10(5):309–10.
26.
Castilho JL, Levi JE, Luz PM, Cambou MC, Vanni T, de Andrade A, et al. A cross-sectional study of high-risk human papillomavirus clustering and cervical outcomes in HIV-infected women in Rio de Janeiro, Brazil. BMC Cancer. 2015;15:478.CrossRefPubMedPubMedCentral
27.
28.
Brismar-Wendel S, Froberg M, Hjerpe A, Andersson S, Johansson B. Age-specific prevalence of HPV genotypes in cervical cytology samples with equivocal or low-grade lesions. Br J Cancer. 2009;101(3):511–7.CrossRefPubMedPubMedCentral
29.
Arbyn M, Verdoodt F, Snijders PJ, Verhoef VM, Suonio E, Dillner L, et al. Accuracy of human papillomavirus testing on self-collected versus clinician-collected samples: a meta-analysis. Lancet Oncol. 2014;15(2):172–83.CrossRefPubMed
30.
Mayrand MH, Duarte-Franco E, Rodrigues I, Walter SD, Hanley J, Ferenczy A, et al. Human papillomavirus DNA versus Papanicolaou screening tests for cervical cancer. N Engl J Med. 2007;357(16):1579–88.CrossRefPubMed
31.
Sankaranarayanan R, Nene BM, Shastri SS, Jayant K, Muwonge R, Budukh AM, et al. HPV screening for cervical cancer in rural India. N Engl J Med. 2009;360(14):1385–94.CrossRefPubMed
32.
Ronco G, Giorgi-Rossi P, Carozzi F, Confortini M, Dalla Palma P, Del Mistro A, et al. Efficacy of human papillomavirus testing for the detection of invasive cervical cancers and cervical intraepithelial neoplasia: a randomised controlled trial. Lancet Oncol. 2010;11(3):249–57.CrossRefPubMed
33.
Sandri MT, Riggio D, Salvatici M, Passerini R, Zorzino L, Boveri S, et al. Typing of human papillomavirus in women with cervical lesions: prevalence and distribution of different genotypes. J Med Virol. 2009;81(2):271–7.CrossRefPubMed
34.
Vidal AC, Murphy SK, Hernandez BY, Vasquez B, Bartlett JA, Oneko O, et al. Distribution of HPV genotypes in cervical intraepithelial lesions and cervical cancer in Tanzanian women. Infect Agent Cancer. 2011;6(1):20.CrossRefPubMedPubMedCentral
35.
36.
Dalstein V, Merlin S, Bali C, Saunier M, Dachez R, Ronsin C. Analytical evaluation of the PapilloCheck test, a new commercial DNA chip for detection and genotyping of human papillomavirus. J Virol Methods. 2009;156(1–2):77–83.CrossRefPubMed
37.
38.
de Villiers EM, Fauquet C, Broker TR, Bernard HU, zur Hausen H. Classification of papillomaviruses. Virology. 2004;324(1):17–27.CrossRefPubMed
39.
Capra G, Giovannelli L, Bellavia C, Migliore MC, Caleca MP, Perino A, et al. HPV genotype prevalence in cytologically abnormal cervical samples from women living in south Italy. Virus Res. 2008;133(2):195–200.CrossRefPubMed
40.
Bosch FX, Lorincz A, Munoz N, Meijer CJ, Shah KV. The causal relation between human papillomavirus and cervical cancer. J Clin Pathol. 2002;55(4):244–65.CrossRefPubMedPubMedCentral
41.
Trottier H, Franco EL. The epidemiology of genital human papillomavirus infection. Vaccine. 2006;24 Suppl 1:S1–15.PubMed
42.
Bosch FX, Burchell AN, Schiffman M, Giuliano AR, de Sanjose S, Bruni L, et al. Epidemiology and natural history of human papillomavirus infections and type-specific implications in cervical neoplasia. Vaccine. 2008;26 Suppl 10:K1–16.CrossRefPubMed
43.
de Ona M, Alvarez-Arguelles ME, Torrents M, Villa L, Rodriguez-Feijoo A, Palacio A, et al. Prevalence, evolution, and features of infection with human papillomavirus: a 15-year longitudinal study of routine screening of a women population in the north of Spain. J Med Virol. 2010;82(4):597–604.CrossRefPubMed
44.
Delgado D, Marin JM, de Diego J, Guerra S, Gonzalez B, Barrios JL, et al. Human papillomavirus (HPV) genotype distribution in women with abnormal cervical cytology in the Basque Country, Spain. Enferm Infecc Microbiol Clin. 2012;30(5):230–5.CrossRefPubMed
45.
Martin P, Kilany L, Garcia D, Lopez-Garcia AM, Martin-Azana MJ, Abraira V, et al. Human papillomavirus genotype distribution in Madrid and correlation with cytological data. BMC Infect Dis. 2011;11:316.CrossRefPubMedPubMedCentral
46.
Schopp B, Holz B, Zago M, Stubenrauch F, Petry KU, Kjaer SK, et al. Evaluation of the performance of the novel PapilloCheck HPV genotyping test by comparison with two other genotyping systems and the HC2 test. J Med Virol. 2010;82(4):605–15.CrossRefPubMed
47.
Ciapponi A, Bardach A, Glujovsky D, Gibbons L, Picconi MA. Type-specific HPV prevalence in cervical cancer and high-grade lesions in Latin America and the Caribbean: systematic review and meta-analysis. PLoS One. 2011;6(10):e25493.CrossRefPubMedPubMedCentral
48.
de Oliveira CM, Fregnani JH, Carvalho JP, Longatto-Filho A, Levi JE. Human papillomavirus genotypes distribution in 175 invasive cervical cancer cases from Brazil. BMC Cancer. 2013;13:357.CrossRefPubMedPubMedCentral
49.
Argyri E, Papaspyridakos S, Tsimplaki E, Michala L, Myriokefalitaki E, Papassideri I, et al. A cross sectional study of HPV type prevalence according to age and cytology. BMC Infect Dis. 2013;13:53.CrossRefPubMedPubMedCentral
50.
Vieira L, Almeida A. The cytology and DNA detection by the PapilloCheck((R)) test in the diagnosis of human papillomavirus infection. Eur J Microbiol Immunol (Bp). 2013;3(1):61–7.CrossRef
51.
Stoler MH, Castle PE, Solomon D, Schiffman M. The expanded use of HPV testing in gynecologic practice per ASCCP-guided management requires the use of well-validated assays. Am J Clin Pathol. 2007;127(3):335–7.CrossRefPubMed
52.
Solomon D. Chapter 14: role of triage testing in cervical cancer screening. J Natl Cancer Inst Monogr. 2003;31:97–101.CrossRefPubMed
53.
Schiffman M, Wentzensen N, Wacholder S, Kinney W, Gage JC, Castle PE. Human papillomavirus testing in the prevention of cervical cancer. J Natl Cancer Inst. 2011;103(5):368–83.CrossRefPubMedPubMedCentral
54.
Cuzick J, Mayrand MH, Ronco G, Snijders P, Wardle J. Chapter 10: new dimensions in cervical cancer screening. Vaccine. 2006;24 Suppl 3:S3/90–7.
55.
Ferreccio C, Barriga MI, Lagos M, Ibanez C, Poggi H, Gonzalez F, et al. Screening trial of human papillomavirus for early detection of cervical cancer in Santiago. Chile Int J Cancer. 2013;132(4):916–23.CrossRefPubMed
56.
Castellsague X. Natural history and epidemiology of HPV infection and cervical cancer. Gynecol Oncol. 2008;110(3 Suppl 2):S4–7.CrossRefPubMed
57.
Castle PE, Solomon D, Wheeler CM, Gravitt PE, Wacholder S, Schiffman M. Human papillomavirus genotype specificity of hybrid capture 2. J Clin Microbiol. 2008;46(8):2595–604.CrossRefPubMedPubMedCentral
58.
Guo M, Hu L, Baliga M, He Z, Hughson MD. The predictive value of p16(INK4a) and hybrid capture 2 human papillomavirus testing for high-grade cervical intraepithelial neoplasia. Am J Clin Pathol. 2004;122(6):894–901.CrossRefPubMed
59.
60.
Peevor R, Bowden S, Jones J, Fiander AN, Hibbitts S. Human Papillomavirus negative but dyskaryotic cervical cytology: re-analysis of molecular testing. J Clin Virol. 2009;44(4):322–4.CrossRefPubMed
61.
Gray SH, Walzer TB. New strategies for cervical cancer screening in adolescents. Curr Opin Pediatr. 2004;16(4):344–9.CrossRefPubMed
62.
Mejlhede N, Bonde J, Fomsgaard A. High frequency of multiple HPV types in cervical specimens from Danish women. APMIS. 2009;117(2):108–14.CrossRefPubMed
63.
Gonzalez-Bosquet E, Esteva C, Munoz-Almagro C, Ferrer P, Perez M, Lailla JM. Identification of vaccine human papillomavirus genotypes in squamous intraepithelial lesions (CIN2-3). Gynecol Oncol. 2008;111(1):9–12.CrossRefPubMed
64.
Sjoeborg KD, Trope A, Lie AK, Jonassen CM, Steinbakk M, Hansen M, et al. HPV genotype distribution according to severity of cervical neoplasia. Gynecol Oncol. 2010;118(1):29–34.CrossRefPubMed
65.
Wright Jr TC, Stoler MH, Behrens CM, Apple R, Derion T, Wright TL. The ATHENA human papillomavirus study: design, methods, and baseline results. Am J Obstet Gynecol. 2012;206(1):46 e1–e11.CrossRefPubMed
66.
Halfon P, Lindemann ML, Raimondo A, Ravet S, Camus C, Khiri H, et al. HPV genotype distribution according to severity of cervical neoplasia using the Digene HPV genotyping LQ test. Arch Virol. 2013;158(6):1143–9.68.CrossRefPubMedPubMedCentral
67.
Wheeler CM, Hunt WC, Cuzick J, Langsfeld E, Pearse A, Montoya GD, et al. A population-based study of human papillomavirus genotype prevalence in the United States: baseline measures prior to mass human papillomavirus vaccination. Int J Cancer. 2013;132(1):198–207.CrossRefPubMed
68.
Chatterjee A. The next generation of HPV vaccines: nonavalent vaccine V503 on the horizon. Expert Rev Vaccines. 2014;13(11):1279–90.CrossRefPubMed
69.
Malagon T, Drolet M, Boily MC, Franco EL, Jit M, Brisson J, et al. Cross-protective efficacy of two human papillomavirus vaccines: a systematic review and meta-analysis. Lancet Infect Dis. 2012;12(10):781–9.CrossRefPubMed
70.
Halec G, Alemany L, Lloveras B, Schmitt M, Alejo M, Bosch FX, et al. Pathogenic role of the eight probably/possibly carcinogenic HPV types 26, 53, 66, 67, 68, 70, 73 and 82 in cervical cancer. J Pathol. 2014;234(4):441–51.CrossRefPubMed
Metadata
Title
HPV genotype distribution in Brazilian women with and without cervical lesions: correlation to cytological data
Authors
Toni Ricardo Martins
Cristina Mendes de Oliveira
Luciana Reis Rosa
Cristiane de Campos Centrone
Célia Luiza Regina Rodrigues
Luisa Lina Villa
José Eduardo Levi
Publication date
01-12-2016
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2016
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/s12985-016-0594-3

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