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Malaria Journal
Published in: Malaria Journal 1/2015

Open Access 01-12-2015 | Research

Plasmodium transmission blocking activities of Vernonia amygdalina extracts and isolated compounds

Authors: Solomon M Abay, Leonardo Lucantoni, Nisha Dahiya, Geme Dori, Edson G Dembo, Fulvio Esposito, Guilio Lupidi, Sonny Ogboi, Robert K Ouédraogo, Annamaria Sinisi, Orazio Taglialatela-Scafati, R Serge Yerbanga, Massimo Bramucci, Luana Quassinti, Jean Bosco Ouédraogo, George Christophides, Annette Habluetzel

Published in: Malaria Journal | Issue 1/2015

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Abstract

Background

Medicinal plants are a validated source for discovery of new leads and standardized herbal medicines. The aim of this study was to assess the activity of Vernonia amygdalina leaf extracts and isolated compounds against gametocytes and sporogonic stages of Plasmodium berghei and to validate the findings on field isolates of Plasmodium falciparum.

Methods

Aqueous (Ver-H2O) and ethanolic (Ver-EtOH) leaf extracts were tested in vivo for activity against sexual and asexual blood stage P. berghei parasites. In vivo transmission blocking effects of Ver-EtOH and Ver-H2O were estimated by assessing P. berghei oocyst prevalence and density in Anopheles stephensi mosquitoes. Activity targeting early sporogonic stages (ESS), namely gametes, zygotes and ookinetes was assessed in vitro using P. berghei CTRPp.GFP strain. Bioassay guided fractionation was performed to characterize V. amygdalina fractions and molecules for anti-ESS activity. Fractions active against ESS of the murine parasite were tested for ex vivo transmission blocking activity on P. falciparum field isolates. Cytotoxic effects of extracts and isolated compounds vernolide and vernodalol were evaluated on the human cell lines HCT116 and EA.hy926.

Results

Ver-H2O reduced the P. berghei macrogametocyte density in mice by about 50% and Ver-EtOH reduced P. berghei oocyst prevalence and density by 27 and 90%, respectively, in An. stephensi mosquitoes. Ver-EtOH inhibited almost completely (>90%) ESS development in vitro at 50 μg/mL. At this concentration, four fractions obtained from the ethylacetate phase of the methanol extract displayed inhibitory activity >90% against ESS. Three tested fractions were also found active against field isolates of the human parasite P. falciparum, reducing oocyst prevalence in Anopheles coluzzii mosquitoes to one-half and oocyst density to one-fourth of controls. The molecules and fractions displayed considerable cytotoxicity on the two tested cell-lines.

Conclusions

Vernonia amygdalina leaves contain molecules affecting multiple stages of Plasmodium, evidencing its potential for drug discovery. Chemical modification of the identified hit molecules, in particular vernodalol, could generate a library of druggable sesquiterpene lactones. The development of a multistage phytomedicine designed as preventive treatment to complement existing malaria control tools appears a challenging but feasible goal.
Appendix
Available only for authorised users
Literature
1.
WHO (2011) World Malaria Report 2011. World Health Organization, Geneva
2.
WHO (2014) Fact Sheet No. 94. World Health Organization, Geneva
3.
Okell LC, Drakeley CJ, Ghani AC, Bousema T, Sutherland CJ (2008) Reduction of transmission from malaria patients by artemisinin combination therapies: a pooled analysis of six randomized trials. Malar J 7:125PubMedCentralPubMedCrossRef
4.
White NJ (2013) Primaquine to prevent transmission of falciparum malaria. Lancet Infect Dis 13:175–181PubMedCrossRef
5.
Abay SM (2013) Blocking malaria transmission to Anopheles mosquitoes using artemisinin derivatives and primaquine: a systematic review and meta-analysis. Parasit Vectors 6:278PubMedCentralPubMedCrossRef
6.
Wilairatana P, Krudsood S, Tangpukdee N (2010) Appropriate time for primaquine treatment to reduce Plasmodium falciparum transmission in hypoendemic areas. Korean J Parasitol 48:179–182PubMedCentralPubMedCrossRef
7.
World Health Organization (2012) Updated WHO policy recommendation (October 2012) single dose primaquine as a gametocytocide in Plasmodium falciparum Malaria. 2012(October)
8.
Eziefula A, Gosling R, Hwang J, Hsiang M, Bousema T, von Seidlein L et al (2012) Rationale for short course primaquine in Africa to interrupt malaria transmission. Malar J 11:360PubMedCentralPubMedCrossRef
9.
Achan J, Talisuna AO, Erhart A, Yeka A, Tibenderana JK, Baliraine FN et al (2011) Quinine, an old anti-malarial drug in a modern world: role in the treatment of malaria. Malar J 10:144PubMedCentralPubMedCrossRef
10.
Weathers PJ, Arsenault PR, Covello PS, Mcmickle A, Teoh KH, Reed DW (2012) Artemisinin production in Artemisia annua: studies in planta and results of a novel delivery method for treating malaria and other neglected diseases. Phytochem Rev 10:173–183CrossRef
11.
Pukrittayakamee S, Chotivanich K, Clemens R, Looareesuwan S, White NJ, Chantra A (2004) Activities of artesunate and primaquine against asexual- and sexual-stage parasites in falciparum malaria. Antimicrob Agents Chemother 48:1329–1334PubMedCentralPubMedCrossRef
12.
Lucantoni L, Yerbanga RS, Lupidi G, Pasqualini L, Esposito F, Habluetzel A (2010) Transmission blocking activity of a standardized neem (Azadirachta indica) seed extract on the rodent malaria parasite Plasmodium berghei in its vector Anopheles stephensi. Malar J 9:66PubMedCentralPubMedCrossRef
13.
Kleeberg H (2004) Neem based products: registration requirement, regulatory processes and global implications. In: Koul O, Wahab S (eds) Neem Today millenium. Kluwater Academic Publishers, New York, p 114
14.
Jones IW, Denholm AA, Ley SV, Lovell H, Wood A, Sinden RE (1994) Sexual development of malaria parasites is inhibited in vitro by the neem extract azadirachtin, and its semi-synthetic analogues. FEMS Microbiol Lett 120:267–273PubMedCrossRef
15.
Yerbanga R, Lucantoni L, Ouédraogo R, Da DF, Yaméogo K, Churcher T et al (2014) Transmission blocking activity of Azadirachta indica and Guiera senegalensis extracts on the sporogonic development of Plasmodium falciparum field isolates in Anopheles coluzzii mosquitoes. Parasit Vectors 7:185PubMedCentralPubMedCrossRef
16.
Adera T (2003) Beliefs and traditional treatment of malaria in Kishe settlement area, southwest Ethiopia. Ethiop Med J 41:25–34PubMed
17.
Willcox M (2011) Improved traditional phytomedcines in current use for the clinical treatment of malaria. Planta Med 77:662–671PubMedCrossRef
18.
Dori GU (2011) Investigating into South Ethiopian folklore medicine employed for malaria intervention and quantitative method for screening drugs/vaccines acting on pre-erythrocytic stage of Plasmodia. Ph.D. thesis, University of Camerino
19.
Gedif T, Hahn HJ (2002) Treatment of malaria in Ethiopian folk medicine. Trop Dr 32:206–209
20.
Godesso A (2008) Community’s perceptions of malaria and the underlying interventions for its management and control in Jimma town, Oromiya National Regional State. Master’s thesis, Addis Ababa University
21.
Toyang N, Verpoorte R (2013) A review of the medicinal potentials of plants of the genus Vernonia (Asteraceae). J Ethnopharmacol 146:681–723PubMedCrossRef
22.
Lacroix D, Prado S, Kamoga D, Kasenene J, Namukobe J, Krief S et al (2011) Antiplasmodial and cytotoxic activities of medicinal plants traditionally used in the village of Kiohima, Uganda. J Ethnopharmacol 133:850–855PubMedCrossRef
23.
Masaba SC (2000) The antimalarial activity of Vernonia amygdalina Del (Compositae). Trans R Soc Trop Med Hyg 94:694–695PubMedCrossRef
24.
Omoregie ES, Pal A, Darokar MP, Chanda D, Sisodia B (2010) In vitro and in vivo antiplasmodial activity and cytotoxicity of extracts from Vernonia amygdalina Del. leaves. Malar J 9(Suppl 2):P30PubMedCentralCrossRef
25.
Tona L, Ngimbi NP, Tsakala M, Mesia K, Cimanga K, Apers S et al (1999) Antimalarial activity of 20 crude extracts from nine African medicinal plants used in Kinshasa, Congo. J Ethnopharmacol 68:193–203PubMedCrossRef
26.
Tona L, Cimanga RK, Mesia K, Musuamba CT, De Bruyne T, Apers S et al (2004) In vitro antiplasmodial activity of extracts and fractions from seven medicinal plants used in the Democratic Republic of Congo. J Ethnopharmacol 93:27–32PubMedCrossRef
27.
Abosi AAO, Raseroka BHB (2003) In vivo antimalarial activity of Vernonia amygdalina. Br J Biomed Sci 60:89–91PubMed
28.
Iwalokun BA (2008) Enhanced antimalarial effects of chloroquine by aqueous Vernonia amygdalina leaf extract in mice infected with chloroquine resistant and sensitive Plasmodium berghei strains. Afr Health Sci 8:25–35PubMedCentralPubMed
29.
Melariri P, Campbell W, Etusim P, Smith P (2011) In vitro and in vivo antiplasmodial activities of extracts of Cymbopogon citratus Staph and Vernonia amygdalina Delile leaves. J Nat Prod 4:164–172
30.
Njan AA, Adzu B, Agaba AG, Byarugaba D, Díaz-Llera S, Bangsberg DR (2008) The analgesic and antiplasmodial activities and toxicology of Vernonia amygdalina. J Med Food 11:574–581PubMedCrossRef
31.
Challand S, Willcox M (2009) A clinical trial of the traditional medicine Vernonia amygdalina in the treatment of uncomplicated malaria. J Altern Complement Med 15:1231–1237PubMedCrossRef
32.
Erasto P, Grierson DS, Afolayan AJ (2006) Bioactive sesquiterpene lactones from the leaves of Vernonia amygdalina. J Ethnopharmacol 106:117–120PubMedCrossRef
33.
Luo X, Jiang Y, Fronczek F, Lin C, Izevbigie E, Lee K et al (2011) Isolation and structure determination of a sesquiterpene lactone (vernodalinol) from Vernonia amygdalina extracts. Pharm Biol 49:464–470PubMedCentralPubMedCrossRef
34.
Owoeye O, Yousuf S, Akhtar M, Qamar K, Dar A, Farombi E et al (2010) Another anticancer elemanolide from Vernonia amygdalina Del. Int J Biol Chem Sci 4:226–234
35.
Kupchan S, Hemingway R, Karim A, Werner D (1969) Tumor inhibitors. XLVII. Vernodalin and vernomygdin. Two new cytotoxic sesquiterpene lactones from Vernonia amygdalina Del. J Org Chem 34:3908–3911PubMedCrossRef
36.
Jisaka M, Ohigashi H, Takagaki T, Nozaki H, Tada T, Hirota M et al (1992) Bitter steroid glucosides, vernoniosides A1, A2, and A3, and related B1 from a possible medicinal plant, Vernonia amygdalina, used by wild chimpanzees. Tetrahedron 48:625–632CrossRef
37.
Igile GO, Oleszek W, Burda S, Jurzysta M (1994) Flavonoids from Vernonia amygdalina and their antioxidant ativities. J Agric Food Chem 42:2445–2448CrossRef
38.
Laekeman GM, Mertens J, Totté J, Bult H, Vlietinck AJ, Herman AG (1983) Isolation and pharmacological characterization of vernolepin. J Nat Prod 46:161–169PubMedCrossRef
39.
Chukwujekwu JC, Lategan CA, Smith PJ, Van Heerden FR, Van Staden J (2009) Antiplasmodial and cytotoxic activity of isolated sesquiterpene lactones from the acetone leaf extract of Vernonia colorata. S Afr J Bot 75:176–179CrossRef
40.
Ohigashi H, Huffman AM, Izutsu D, Koshimizu K, Kawanaka M, Sugiyama H et al (1994) Toward the chemical ecology of medicinal plant use in chimpanzees: The case of Vernonia amygdalina, a plant used by wild chimpanzees possibly for parasite-related diseases. J Chem Ecol 20:541–553PubMedCrossRef
41.
Asaka Y, Kubota T, Kukarni A (1977) Studies on a bitter principle from Vernonia anthelmintica. Phytochemistry 16:1838–1839CrossRef
42.
Rabe T, Mullholland D, van Staden J (2002) Isolation and identification of antibacterial compounds from Vernonia colorata leaves. J Ethnopharmacol 80:91–94PubMedCrossRef
43.
Orjuela-Sánchez P, Duggan E, Nolan J, Frangos JA, Carvalho LJM (2012) A lactate dehydrogenase ELISA-based assay for the in vitro determination of Plasmodium berghei sensitivity to anti-malarial drugs. Malar J 11:366PubMedCentralPubMedCrossRef
44.
Lundie RJ, Koning-Ward TF, Davey GM, Nie CQ, Hansen DS, Lau LS et al (2008) Blood-stage Plasmodium infection induces CD8+ T lymphocytes to parasite-expressed antigens, largely regulated by CD8alpha+ dendritic cells. Proc Natl Acad Sci USA 105:14509–14514PubMedCentralPubMedCrossRef
45.
46.
Vlachou D, Zimmermann T, Cantera R, Janse CJ, Waters AP, Kafatos FC (2004) Real-time, in vivo analysis of malaria ookinete locomotion and mosquito midgut invasion. Cell Micro 6:671–685CrossRef
47.
Coetzee M, Hunt RH, Wilkerson R, Della TA, Coulibaly MB, Besansky NJ (2013) Anopheles coluzzii and Anopheles amharicus, new members of the Anopheles gambiae complex. Zootaxa 3619:246–274PubMedCrossRef
48.
Blagborough AM, Churcher TS, Upton LM, Ghani AC, Gething PW, Sinden RE (1812) Transmission-blocking interventions eliminate malaria from laboratory populations. Nat Commun 2013:4
49.
Kiszewski AE (2010) Blocking Plasmodium falciparum malaria transmission with drugs: the gametocytocidal and sporontocidal properties of current and prospective antimalarials. Pharmaceuticals 4:44–68PubMedCentralCrossRef
50.
Chotivanich K, Sattabongkot J, Udomsangpetch R, Looareesuwan S, Day NPJ, Coleman RE et al (2006) Transmission-blocking activities of quinine, primaquine, and artesunate. Antimicrob Agents Chemother 50:1927–1930PubMedCentralPubMedCrossRef
51.
Coleman RE, Nath AK, Schneider I, Song GH, Klein TA, Milhous WK (1994) Prevention of sporogony of Plasmodium falciparum and P. berghei in Anopheles stephensi mosquitoes by transmission-blocking antimalarials. Am J Trop Med Hyg 50:646–653PubMed
52.
Tepongning RN, Lucantoni L, Nasuti CC, Dori GU, Yerbanga SR, Lupidi G et al (2011) Potential of a Khaya ivorensis—Alstonia boonei extract combination as antimalarial prophylactic remedy. J Ethnopharmacol 137:743–751PubMedCrossRef
53.
Jarvis B (2008) Statistical aspects of the microbiological examinations of foods, 2nd edn. Elsevier, Amsterdam, p 8
54.
Delves MJ, Ramakrishnan C, Blagborough AM, Leroy D, Wells TNC, Sinden RE (2012) A high-throughput assay for the identification of malarial transmission-blocking drugs and vaccines. Int J Parasitol 42:999–1006PubMedCrossRef
55.
Torres JA, Rodriguez MH, Rodriguez MC, Hernandez-Hernandez FC (2005) Plasmodium berghei: effect of protease inhibitors during gametogenesis and early zygote development. Exp Parasitol 111:255–259PubMedCrossRef
56.
Delves MJ, Ruecker A, Straschil U, Lelièvre J, Marques S, López-Barragán MJ et al (2013) Male and female Plasmodium falciparum mature gametocytes show different responses to antimalarial drugs. Antimicrob Agents Chemother 57:3268–3274PubMedCentralPubMedCrossRef
57.
Pradel G (2007) Proteins of the malaria parasite sexual stages: expression, function and potential for transmission blocking strategies. Parasitology 134:1911–1929PubMedCrossRef
58.
Rupp I, Bosse R, Schirmeister T, Pradel G (2008) Effect of protease inhibitors on exflagellation in Plasmodium falciparum. Mol Bochem Parasitol 158:208–212CrossRef
59.
Rostorfer HH (1949) Comparison of methods for measurement of avian hemoglobin. J Biol Chem 180:901–911PubMed
60.
Martínez A, Rajapakse CSK, Naoulou B, Kopkalli Y, Sánchez-delgado RA (2008) The mechanism of antimalarial action of the ruthenium (II)-chloroquine complex [RuCl2(CQ)]2. J Biol Inorg Chem 13:703–712PubMedCentralPubMedCrossRef
61.
Mokoka TA, McGaw LJ, Mdee LK, Bagla VP, Iwalewa EO, Eloff JN (2013) Antimicrobial activity and cytotoxicity of triterpenes isolated from leaves of Maytenusundata (Celastraceae). BMC Complement Altern Med 13:111PubMedCentralPubMedCrossRef
62.
Bousema T, Dinglasan RR, Morlais I, Gouagna LC, van Warmerdam T, Awono-Ambene PH et al (2012) Mosquito feeding assays to determine the infectiousness of naturally infected Plasmodium falciparum gametocyte carriers. PLoS One 7:e42821PubMedCentralPubMedCrossRef
63.
Mehra N, Bhasin V (1993) In vitro gametocytocidal activity of artemisinin and its derivatives on Plasmodium falciparum. Jpn J Med Sci Biol 46:37–43PubMedCrossRef
64.
Bousema T, Okell L, Shekalaghe S, Griffin JT, Omar S, Sawa P et al (2010) Revisiting the circulation time of Plasmodium falciparum gametocytes: molecular detection methods to estimate the duration of gametocyte carriage and the effect of gametocytocidal drugs. Malar J 9:136PubMedCentralPubMedCrossRef
65.
66.
Adebayo OL, James A, Kasim SB, Jagri OP (2014) Leaf extracts of Vernonia amygdalina Del. from Northern Ghana contain bioactive agents that inhibit the growth of some beta-lactamase producing bacteria in vitro. BR. J Pharm Res 4:192–202
67.
Imaga NOA, Bamigbetan DO (2013) In vivo biochemical assessment of aqueous extracts of Vernonia amygdalina (Bitter leaf). Int J Nutr Metab 5:22–27
68.
Akinjogunla O, Ekoi O, Odeyemi A, Odeyemy A (2011) Phytochemical screening and in vitro antibacterial assessment of aqueous leaf extracts of Vernonia amygdalina (Asteraceae) and Ocimum gratissimum (Lamiaceae) on moxifloxacin resistant Escherichia coli isolated from clinical and environmental samples. Nat Sci 9:42–52
69.
Anyasor G, Ogunwenmo K, Ogunnowo A, Alao-Sanni O (2010) Comparative antioxidant phytochemical and proximate analysis of aqueous and methanolic extracts of Vernonia amygdalina and Talinum triangulare.pdf. Pakistan. J Nutr 9:259–264
70.
Ghamba P, Balla H, Goje L, Halidu A, Dauda M (2014) In vitro antimicrobial activities of Vernonia amygdalina on selected clinical isolates. Int J Curr Microbiol Appl Sci 3:1103–1113
71.
Seidel V (2005) Initial and bulk extraction. In: Sarker SD, Latif Z, Gray AI (eds) Natural products isolation, 2nd edn. Humana press Inc., Totowa, p 36
72.
Woods JR, Mo H, Bieberich AA, Alavanja T, Colby DA (2013) Amino-derivatives of the sesquiterpene lactone class of natural products as prodrugs. MedChemComm 4:27–33CrossRef
73.
Jisaka M, Ohigashi H, Takegawa K, Huffman M, Koshimizu K (1993) Antitumoral and antimicrobial activities of bitter sesquiterpene lactone of Vernonia amygdalina, a possible medicinal plant used by wild chimpanzees. Biosci Biotech Biochem 57:833–834CrossRef
74.
Ngbolua KN, Rakotoarimanana H, Rafatro H, Ratsimamanga US, Mudogo V, Mpiana PT et al (2011) Comparative antimalarial and cytotoxic activities of two Vernonia species: Vernonia amygdalina from the Democratic Republic of Congo and Vernonia cinerea subsp vialis endemic to Madagascar. Int J Biol Chem Sci 5:345–353
75.
Omorgie E, Pal A, Sisodia B (2011) In vitro antimalarial and cytotoxic activities of leaf extracts of Vernonia amygdalina (Del.). Niger J Basic Appl Sci 19:121–126
76.
Bogale M, Petros B (1996) Evaluation of the antimalarial activity of some Ethiopian traditional medicinal plants against Plasmodium falciparum in vitro. SINET Ethiop J 19:233
77.
Pedersen MM, Chukwujekwu JC, Lategan CA, van Staden J, Smith PJ, Staerk D (2009) Antimalarial sesquiterpene lactones from Distephanus angulifolius. Phytochemistry 70:601–607PubMedCrossRef
78.
Pink R, Hudson A, Mouriès M-A, Bendig M (2005) Opportunities and challenges in antiparasitic drug discovery. Nat Rev Drug Discov 4:727–740PubMedCrossRef
79.
Pratheeshkumar P, Kuttan G (2011) Effect of vernolide-A, a sesquiterpene lactone from Vernonia cinerea L., on cell-mediated immune response in B16F-10 metastatic melanoma-bearing mice. Immunopharmacol Immunotoxicol 33:533–538PubMedCrossRef
80.
Ibrahim G, Abdurahman M, Ibrahim H, Ibrahim N, Magajii M (2011) Toxicity and analgesic effects of Vernonia amygdalina Del. (Asteraceae) leaf extract on mice. Int J Adv Pharm Biol Sci 1:1–4
81.
Luke UO, Robert AE, Egbung GE (2013) Effect of ethanolic root and twig extracts of Vernonia amygdalina (etidot) on liver function parameters of streptozotocin induced hyperglycaemic and normal wistar rats. Eur Sci J 9:199–211
82.
Nwanjo HU (2005) Efficacy of aqueous leaf extract of Vernonia amygdalina on plasma lipoprotein and oxidative status in diabetic rat models. Niger J Physiol Sci 20:39–42PubMed
83.
Yeap S, Liang W, Beh B, Ho W, Yousr A, Alitheen N (2012) In vivo antidiabetic and acute toxicity of spray-dried Vernonia amygdalina water extract. Int Food Res J 20:613–616
84.
Sha’a K, Oguche S, Watila I, Ikpa T (2011) In vitro antimalarial activity of the extracts of Vernonia amygdalina commonly used in traditional medicine in Nigeria. Sci World J 6:5–9
85.
Akah PA, Alemji JA, Salawu OA, Okoye TC, Offiah NV (2009) Effects of Vernonia amygdalina on biochemical and hematological parameters in diabetic rats. Asian J Med Sci 1:108–113
Metadata
Title
Plasmodium transmission blocking activities of Vernonia amygdalina extracts and isolated compounds
Authors
Solomon M Abay
Leonardo Lucantoni
Nisha Dahiya
Geme Dori
Edson G Dembo
Fulvio Esposito
Guilio Lupidi
Sonny Ogboi
Robert K Ouédraogo
Annamaria Sinisi
Orazio Taglialatela-Scafati
R Serge Yerbanga
Massimo Bramucci
Luana Quassinti
Jean Bosco Ouédraogo
George Christophides
Annette Habluetzel
Publication date
01-12-2015
Publisher
BioMed Central
Published in
Malaria Journal / Issue 1/2015
Electronic ISSN: 1475-2875
DOI
https://doi.org/10.1186/s12936-015-0812-2

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