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Cancer Cell International
Published in: Cancer Cell International 1/2022

Open Access 01-12-2022 | Review

Technique integration of single-cell RNA sequencing with spatially resolved transcriptomics in the tumor microenvironment

Authors: Hailan Yan, Jinghua Shi, Yi Dai, Xiaoyan Li, Yushi Wu, Jing Zhang, Zhiyue Gu, Chenyu Zhang, Jinhua Leng

Published in: Cancer Cell International | Issue 1/2022

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Abstract

Background

The tumor microenvironment contributes to tumor initiation, growth, invasion, and metastasis. The tumor microenvironment is heterogeneous in cellular and acellular components, particularly structural features and their gene expression at the inter-and intra-tumor levels.

Main text

Single-cell RNA sequencing profiles single-cell transcriptomes to reveal cell proportions and trajectories while spatial information is lacking. Spatially resolved transcriptomics redeems this lack with limited coverage or depth of transcripts. Hence, the integration of single-cell RNA sequencing and spatial data makes the best use of their strengths, having insights into exploring diverse tissue architectures and interactions in a complicated network. We review applications of integrating the two methods, especially in cellular components in the tumor microenvironment, showing each role in cancer initiation and progression, which provides clinical relevance in prognosis, optimal treatment, and potential therapeutic targets.

Conclusion

The integration of two approaches may break the bottlenecks in the spatial resolution of neighboring cell subpopulations in cancer, and help to describe the signaling circuitry about the intercommunication and its exact mechanisms in producing different types and malignant stages of tumors.
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Metadata
Title
Technique integration of single-cell RNA sequencing with spatially resolved transcriptomics in the tumor microenvironment
Authors
Hailan Yan
Jinghua Shi
Yi Dai
Xiaoyan Li
Yushi Wu
Jing Zhang
Zhiyue Gu
Chenyu Zhang
Jinhua Leng
Publication date
01-12-2022
Publisher
BioMed Central
Published in
Cancer Cell International / Issue 1/2022
Electronic ISSN: 1475-2867
DOI
https://doi.org/10.1186/s12935-022-02580-4

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