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Cancer Cell International
Published in: Cancer Cell International 1/2020

01-12-2020 | Radiotherapy | Primary research

Baicalin reverses radioresistance in nasopharyngeal carcinoma by downregulating autophagy

Authors: Cong Wang, Yinli Yang, Lining Sun, Jing Wang, Zhansheng Jiang, Yanwei Li, Dongying Liu, Haiyan Sun, Zhanyu Pan

Published in: Cancer Cell International | Issue 1/2020

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Abstract

Background

Radiation resistance is the main cause of recurrence after radiotherapy, and increased autophagy after radiotherapy is related to radiotherapy resistance. This study aims to investigate the reversal effect of baicalin on radioresistance and its related mechanism.

Methods

CCK-8 and flow cytometry were used to detect the effect of proliferation and apoptosis by baicalin. Clone formation test was used to verify the effect of baicalin radiosensitization. Western blot analysis and electron microscopy were employed to observe the effect of baicalin on autophagy.

Results

Compared with the radiation therapy (RT) group, the RT combined baicalin (RT + BA) group showed a significantly low 2 Gy survival fraction of radiation therapy (P < 0.05). LC3-II protein expression in the RT group was significantly higher than which in the RT + BA group (P < 0.05). Electron microscopy showed that more autophagic vacuoles were observed in the RT group than those in the RT + BA group.

Conclusions

Overall, baicalin can reverse the radioresistance of human nasopharyngeal carcinoma CNE-2R cells by downregulating RT-enhanced autophagy.
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Metadata
Title
Baicalin reverses radioresistance in nasopharyngeal carcinoma by downregulating autophagy
Authors
Cong Wang
Yinli Yang
Lining Sun
Jing Wang
Zhansheng Jiang
Yanwei Li
Dongying Liu
Haiyan Sun
Zhanyu Pan
Publication date
01-12-2020
Publisher
BioMed Central
Keyword
Radiotherapy
Published in
Cancer Cell International / Issue 1/2020
Electronic ISSN: 1475-2867
DOI
https://doi.org/10.1186/s12935-020-1107-4

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