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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2017

Open Access 01-12-2017 | Research article

Chemical composition and anti-herpes simplex virus type 1 (HSV-1) activity of extracts from Cornus canadensis

Authors: Serge Lavoie, Isabelle Côté, André Pichette, Charles Gauthier, Michaël Ouellet, Francine Nagau-Lavoie, Vakhtang Mshvildadze, Jean Legault

Published in: BMC Complementary Medicine and Therapies | Issue 1/2017

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Abstract

Background

Many plants of boreal forest of Quebec have been used by Native Americans to treat a variety of microbial infections. However, the antiviral activities of these plants have been seldom evaluated on cellular models to validate their in vitro efficiencies. In this study, Cornus canadensis L. (Cornaceae), a plant used in Native American traditional medicine to treat possible antiviral infections, has been selected for further examination.

Methods

The plant was extracted by decoction and infusion with water, water/ethanol 1:1 and ethanol to obtain extracts similar to those used by Native Americans. The effects of the extracts were tested on herpes simplex virus type-1 (HSV-1) using a plaque reduction assay. Moreover, bioassay-guided fractionation was achieved to isolate bioactive compounds.

Results

Water/ethanol 1:1 infusion of C. canadensis leaves were the most active extracts to inhibit virus absorption with EC50 of about 9 μg mL−1, whereas for direct mode, both extraction methods using water or water/ethanol 1:1 as solvent were relatively similar with EC50 ranging from 11 to 17 μg mL−1. The fractionation led to the identification of active fractions containing hydrolysable tannins. Tellimagrandin I was found the most active compound with an EC50 of 2.6 μM for the direct mode and 5.0 μM for the absorption mode.

Conclusion

Altogether, the results presented in this work support the antiviral activity of Cornus canadensis used in Native American traditional medicine.
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Metadata
Title
Chemical composition and anti-herpes simplex virus type 1 (HSV-1) activity of extracts from Cornus canadensis
Authors
Serge Lavoie
Isabelle Côté
André Pichette
Charles Gauthier
Michaël Ouellet
Francine Nagau-Lavoie
Vakhtang Mshvildadze
Jean Legault
Publication date
01-12-2017
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2017
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-017-1618-2

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