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BMC Pediatrics

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Open Access 01-12-2024 | Respiratory Microbiota | Research

Alterations of gut microbiota in infants with biliary atresia identified by 16S rRNA-sequencing

Authors: Fei Liu, Ru Wei, Xiaogang Xu, Menglong Lan, Boyuan Tao, Zijian Liang, Jixiao Zeng

Published in: BMC Pediatrics | Issue 1/2024

Abstract

Background

Biliary atresia (BA) is a severe neonatal disease with progressive intra- and extra-hepatic bile ducts inflammation and hepatic fibrosis. Characterization of gut microbiome profiles in infants with biliary atresia can provide valuable information and potential disease biomarkers. Our study aims to explore the relationship between gut microbiota and biliary atresia.

Methods

16 S ribosomal RNA (rRNA) gene sequencing was carried out to identify the differences in composition and diversity of gut microbiota between infants with BA and healthy subjects. A total of 31 infants with biliary atresia and 20 healthy subjects were recruited.

Results

The composition of gut microbiota in BA group was significantly different with the normal control group (P < 0.05) and the abundance ratio of Klebsiella/Bifidobacterium showed great potential for identification of BA (P < 0.01). In addition, the differential bacterial taxa were involved in lipid and vitamins metabolism.

Conclusion

Our results could provide potential non-invasive biomarker for identification of biliary atresia and contribute to the treatment in terms of ameliorating microbiota dysbiosis.

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Jiang H, Gao P, Chen H, Zhong Z, Shu M, Zhang Z, et al. The prognostic value of CD8 + and CD45RO + T cells infiltration and Beclin1 expression levels for early postoperative cholangitis of biliary atresia patients after Kasai operation. J Korean Med Sci. 2018;33:e198. https://doi.org/10.3346/jkms.2018.33.e198.CrossRefPubMedPubMedCentral

Song W, Sun LY, Zhu ZJ, Wei L, Qu W, Zeng ZG, et al. Characteristics of gut microbiota in children with biliary atresia after liver transplantation. Front Physiol. 2021;12:704313. https://doi.org/10.3389/fphys.2021.704313.CrossRefPubMedPubMedCentral

Sundaram SS, Mack CL, Feldman AG, Sokol RJ. Biliary atresia: indications and timing of liver transplantation and optimization of pretransplant care. Liver Transpl. 2017;23:96–109. https://doi.org/10.1002/lt.24640.CrossRefPubMedPubMedCentral

Gad EH, Kamel Y, Salem TA, Ali MA, Sallam AN. Short- and long-term outcomes after Kasai operation for type III biliary atresia: twenty years of experience in a single tertiary Egyptian center-A retrospective cohort study. Ann Med Surg. 2021;62:302–14. https://doi.org/10.1016/j.amsu.2021.01.052.CrossRef

Ji Y, Yang K, Zhang X, Jin S, Jiang X, Chen S, et al. The short-term outcome of modified laparoscopic Kasai portoenterostomy for biliary atresia. Surg Endosc. 2021;35:1429–34. https://doi.org/10.1007/s00464-020-07530-7.CrossRefPubMed

Venkat V, Ng VL, Magee JC, Ye W, Hawthorne K, Harpavat S, et al. Modeling outcomes in children with biliary atresia with native liver after 2 years of age. Hepatol Commun. 2020;4:1824–34. https://doi.org/10.1002/hep4.1602.CrossRefPubMedPubMedCentral

Bezerra JA, Wells RG, Mark CL, Karpen SJ, Hoofnagle JH, Doo E, et al. Biliary atresia: clinical and research challenges for the twenty-first century. Hepatology. 2018;68:1163–73. https://doi.org/10.1002/hep.29905.CrossRefPubMed

Hukkinen M, Kerola A, Lohi J, Jahnukainen T, Heikkila P, Pakarinen MP. Very low bilirubin after portoenterostomy improves survival of the native liver in patients with biliary atresia by deferring liver fibrogenesis. Surgery. 2019;165:843–50. https://doi.org/10.1016/j.surg.2018.10.032.CrossRefPubMed

Khayat A, Alamri AM, Saadah OI. Outcomes of late Kasai portoenterostomy in biliary atresia: a single-center experience. J Int Med Res. 2021;49:3000605211012596. https://doi.org/10.1177/03000605211012596.CrossRefPubMed

10.

Ryon EL, Parreco JP, Sussman MS, Quiroz HJ, Willobee BA, Perez EA, et al. Drivers of hospital readmission and early liver transplant after Kasai portoenterostomy. J Surg Res. 2020;256:48–55. https://doi.org/10.1016/j.jss.2020.06.019.CrossRefPubMed

11.

Ramachandran P, Safwan M, Balaji MS, Unny AK, Akhtarkhavari A, Tamizhvanan V, et al. Early cholangitis after portoenterostomy in children with biliary atresia. J Indian Assoc Pediatr Surg. 2019;24:185–8. https://doi.org/10.4103/jiaps.JIAPS_96_18.CrossRefPubMedPubMedCentral

12.

Yang L, Zhou Y, Xu PP, Mourya R, Lei HY, Cao GQ, et al. Diagnostic accuracy of serum matrix metalloproteinase-7 for biliary atresia. Hepatology. 2018;68:2069–77. https://doi.org/10.1002/hep.30234.CrossRefPubMed

13.

He L, Ming DK, Tam G, Lui VCH, Tam PWH, Chung PHY. Biomarkers for the diagnosis and post–Kasai portoenterostomy prognosis of biliary atresia: a systematic review and meta–analysis. Sci Rep. 2021;11:11692. https://doi.org/10.1038/s41598-021-91072-y.CrossRefPubMedPubMedCentralADS

14.

Brahee DD, Lampl BS. Neonatal diagnosis of biliary atresia: a practical review and update. Pediatr Radiol. 2022;52:685–92. https://doi.org/10.1007/s00247-021-05148-y.CrossRefPubMed

15.

Liu F, Zeng J, Zhu D, Xu X, Lan M, Wang M, et al. PDGFA gene rs9690350 polymorphism increases biliary atresia risk in Chinese children. Biosci Rep. 2020;40:BSR20200068. https://doi.org/10.1042/BSR20200068.CrossRefPubMedPubMedCentral

16.

Jain V, Alexander EC, Burford C, Verma A, Dhawan A. Gut: a potential modifiable risk factor in biliary atresia. J Pediatr Gastroenterol Nutr. 2021;72:184–93. https://doi.org/10.1097/MPG.0000000000002973.CrossRefPubMed

17.

Mentella MC, Scaldaferri F, Pizzoferrato M, Gasbarrini A, Miggiano GAD, Nutrition. IBD and gut microbiota: a review. Nutrients. 2020;12:944. https://doi.org/10.3390/nu12040944.CrossRefPubMedPubMedCentral

18.

Pan Q, Guo F, Huang Y, Li A, Chen S, Chen J, et al. Gut microbiota dysbiosis in systemic lupus erythematosus: novel insights into mechanisms and promising therapeutic strategies. Front Immunol. 2021;12:799788. https://doi.org/10.3389/fimmu.2021.799788.CrossRefPubMedPubMedCentral

19.

Wang J, Qian T, Jiang J, Yang Y, Shen Z, Huang Y, et al. Gut microbial profile in biliary atresia: a case-control study. J Gastroenterol Hepatol. 2020;35:334–42. https://doi.org/10.1111/jgh.14777.CrossRefPubMed

20.

van Wessel D, Nomden M, Bruggink J, de Kleine R, Kurilshikov A, Verkade H, et al. Gut microbiota composition of biliary atresia patients before Kasai portoenterostomy associates with long-term outcome. J Pediatr Gastroenterol Nutr. 2021;73:485–90. https://doi.org/10.1097/MPG.0000000000003234.CrossRefPubMedPubMedCentral

21.

Fligor SC, Hirsch TI, Tsikis ST, Adeola A, Puder M. Current and emerging adjuvant therapies in biliary atresia. Front Pediatr. 2022;10:1007813. https://doi.org/10.3389/fped.2022.1007813.CrossRefPubMedPubMedCentral

22.

Forbes JD, Azad MB, Vehling L, Tun HM, Konya TB, Guttman DS, et al. Association of exposure to formula in the hospital and subsequent infant feeding practices with gut microbiota and risk of overweight in the first year of life. JAMA Pediatr. 2018;172:e181161. https://doi.org/10.1001/jamapediatrics.2018.1161.CrossRefPubMedPubMedCentral

23.

Xiong X, Loo SL, Zhang L, Tanaka MM. Modelling the effect of birth and feeding modes on the development of human gut microbiota. Proc. R. Soc. B. 2021;288:20201810. https://doi.org/10.1098/rspb.2020.1810.

24.

Tessier MEM, Cavallo L, Yeh J, Harpavat S, Hoffman K, Petrosino JF, et al. The fecal microbiome in infants with biliary atresia associates with bile flow after Kasai portoenterostomy. J Pediatr Gastroenterol Nutr. 2020;70:789–95. https://doi.org/10.1097/MPG.0000000000002686.CrossRefPubMed

25.

Sun X, Cai Y, Dai W, Jiang W, Tang W. The difference of gut microbiome in different biliary diseases in infant before operation and the changes after operation. BMC Pediatr. 2022;22:502. https://doi.org/10.1186/s12887-022-03570-1.CrossRefPubMedPubMedCentral

26.

Elaine Chen YF, Lai MW, Tsai CN, Lai JY, Yang YC, Chen SY. Association of gut microbiota composition and copy number variation with Kasai procedure outcomes in infants with biliary atresia. Pediatr Neonatol. 2020;61:238–40. https://doi.org/10.1016/j.pedneo.2019.12.011.CrossRefPubMed

Title: Alterations of gut microbiota in infants with biliary atresia identified by 16S rRNA-sequencing
Authors: Fei Liu
Ru Wei
Xiaogang Xu
Menglong Lan
Boyuan Tao
Zijian Liang
Jixiao Zeng
Publication date: 01-12-2024
Publisher: BioMed Central
Keywords: Respiratory Microbiota
Biomarkers
Published in: BMC Pediatrics / Issue 1/2024
Electronic ISSN: 1471-2431
DOI: https://doi.org/10.1186/s12887-024-04582-9

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Springer Medicine

Alterations of gut microbiota in infants with biliary atresia identified by 16S rRNA-sequencing

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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