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BMC Immunology
Published in: BMC Immunology 1/2020

Open Access 01-12-2020 | Research article

Tertiary lymphoid structure related B-cell IgE isotype switching and secondary lymphoid organ linked IgE production in mouse allergy model

Authors: Dmitrii Borisovich Chudakov, Dmitrii Yuryevich Ryasantsev, Daria Sergeevna Tsaregorotseva, Olga Dmitrievna Kotsareva, Gulnar Vaisovna Fattakhova, Elena Viktorovna Svirshchevskaya

Published in: BMC Immunology | Issue 1/2020

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Abstract

Background

Numerous data obtained by different research laboratories indicate that specific IgE production is triggered independently of specific IgG or IgA ones and so it is not linked to fully matured germinal centers formation in the secondary lymphoid organs. The aim of this study was to clarify whether specific IgE production is triggered by low antigen doses administrated in tertiary tissues enriched by lymphoid structures.

Methods

Ovalbumin (OVA) in different doses (100 ng to 10 μg) was administrated three times a week for 4–5 weeks intraperitoneally (i.p.) or subcutaneously (s.c.) to female BALB/c mice in the wither region which is enriched in fat-associated lymphoid clusters or in the foot pad region not containing them.

Results

OVA-specific IgE was predominantly induced by low but not high antigen doses and only after immunization into the withers. IgE isotype switching was triggered exclusively in the withers adipose tissue but not in the regional lymph nodes while mature IgE expressing cells were observed both in the withers and lymph nodes. Anti-proliferative genotoxic stress inducing drugs shifted the balance from IgG1 towards IgE production.

Conclusions

Tertiary lymphoid structures possess unique environment where B-cell antibody isotype switching to IgE predominantly occurs. This phenomenon is partially explained by hampered proliferation of B-cells in these structures.
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Metadata
Title
Tertiary lymphoid structure related B-cell IgE isotype switching and secondary lymphoid organ linked IgE production in mouse allergy model
Authors
Dmitrii Borisovich Chudakov
Dmitrii Yuryevich Ryasantsev
Daria Sergeevna Tsaregorotseva
Olga Dmitrievna Kotsareva
Gulnar Vaisovna Fattakhova
Elena Viktorovna Svirshchevskaya
Publication date
01-12-2020
Publisher
BioMed Central
Published in
BMC Immunology / Issue 1/2020
Electronic ISSN: 1471-2172
DOI
https://doi.org/10.1186/s12865-020-00376-7

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