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Open Access 01-12-2021 | Migraine | Research article

Identification of disease- and headache-specific mediators and pathways in migraine using blood transcriptomic and metabolomic analysis

Authors: Timea Aczél, Tamás Körtési, József Kun, Péter Urbán, Witold Bauer, Róbert Herczeg, Róbert Farkas, Krisztián Kovács, Barna Vásárhelyi, Gellért B. Karvaly, Attila Gyenesei, Bernadett Tuka, János Tajti, László Vécsei, Kata Bölcskei, Zsuzsanna Helyes

Published in: The Journal of Headache and Pain | Issue 1/2021

Abstract

Background

Recent data suggest that gene expression profiles of peripheral white blood cells can reflect changes in the brain. We aimed to analyze the transcriptome of peripheral blood mononuclear cells (PBMC) and changes of plasma metabolite levels of migraineurs in a self-controlled manner during and between attacks.

Methods

Twenty-four patients with migraine were recruited and blood samples were collected in a headache-free (interictal) period and during headache (ictal) to investigate disease- and headache-specific alterations. Control samples were collected from 13 age- and sex-matched healthy volunteers. RNA was isolated from PBMCs and single-end 75 bp RNA sequencing was performed using Illumina NextSeq 550 instrument followed by gene-level differential expression analysis. Functional analysis was carried out on information related to the role of genes, such as signaling pathways and biological processes. Plasma metabolomic measurement was performed with the Biocrates MxP Quant 500 Kit.

Results

We identified 144 differentially-expressed genes in PBMCs between headache and headache-free samples and 163 between symptom-free patients and controls. Network analysis revealed that enriched pathways included inflammation, cytokine activity and mitochondrial dysfunction in both headache and headache-free samples compared to controls. Plasma lactate, succinate and methionine sulfoxide levels were higher in migraineurs while spermine, spermidine and aconitate were decreased during attacks.

Conclusions

It is concluded that enhanced inflammatory and immune cell activity, and oxidative stress can play a role in migraine susceptibility and headache generation.

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Title: Identification of disease- and headache-specific mediators and pathways in migraine using blood transcriptomic and metabolomic analysis
Authors: Timea Aczél
Tamás Körtési
József Kun
Péter Urbán
Witold Bauer
Róbert Herczeg
Róbert Farkas
Krisztián Kovács
Barna Vásárhelyi
Gellért B. Karvaly
Attila Gyenesei
Bernadett Tuka
János Tajti
László Vécsei
Kata Bölcskei
Zsuzsanna Helyes
Publication date: 01-12-2021
Publisher: Springer Milan
Keywords: Migraine
Cytokines
Headache
Published in: The Journal of Headache and Pain / Issue 1/2021
Print ISSN: 1129-2369
Electronic ISSN: 1129-2377
DOI: https://doi.org/10.1186/s10194-021-01285-9

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Identification of disease- and headache-specific mediators and pathways in migraine using blood transcriptomic and metabolomic analysis

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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