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Arthritis Research & Therapy
Published in: Arthritis Research & Therapy 3/2011

Open Access 01-06-2011 | Research article

Analysis of IL-17+cells in facet joints of patients with spondyloarthritis suggests that the innate immune pathway might be of greater relevance than the Th17-mediated adaptive immune response

Authors: Heiner Appel, René Maier, Peihua Wu, Rebecca Scheer, Axel Hempfing, Ralph Kayser, Andreas Thiel, Andreas Radbruch, Christoph Loddenkemper, Joachim Sieper

Published in: Arthritis Research & Therapy | Issue 3/2011

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Abstract

Introduction

In this study, we analysed the number of IL-17+ cells in facet joints, in the peripheral blood (PB) and synovial fluid (SF) of spondyloarthritis (SpA) patients and compared these results with those of patients with other rheumatic diseases and controls.

Methods

Immunohistochemical analysis of IL-17+ cells was performed in facet joints of 33 ankylosing spondylitis (AS) patients and compared with data from 20 osteoarthritis (OA) patients. The frequency of IL-17+CD4+ T cells in PB and SF of SpA patients (PB n = 30, SF n = 11), rheumatoid arthritis (RA) patients (PB n = 14, SF n = 7), OA patients (PB n = 10) and healthy controls (PB n = 12) was analysed after stimulation with Staphylococcus aureus Enterotoxin B and phorbol 12-myristate 13-acetate/ionomycin and quantified by flow cytometry.

Results

In AS facet joints, the frequency of IL-17-secreting cells was significantly higher than in samples obtained from OA patients (P < 0.001), with a slight predominance of IL-17+ cells among the mononuclear cells (61.5% ± 14.9%) compared to cells with polysegmental nuclei. Immunofluorescence microscopy revealed that the majority of IL-17+ cells were myeloperoxidase-positive (35.84 ± 13.06/high-power field (HPF) and CD15+ neutrophils (24.25 ± 10.36/HPF), while CD3+ T cells (0.51 ± 0.49/HPF) and AA-1+ mast cells (2.28 ± 1.96/HPF) were less often IL-17-positive. The frequency of IL-17+CD4+ T cells in the PB and SF of SpA patients did not differ significantly compared to RA patients, OA patients or healthy controls.

Conclusions

Our data suggest an important role for IL-17 in the inflammatory processes in AS. However, the innate immune pathway might be of greater relevance than the Th17-mediated adaptive immune response.
Appendix
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Metadata
Title
Analysis of IL-17+cells in facet joints of patients with spondyloarthritis suggests that the innate immune pathway might be of greater relevance than the Th17-mediated adaptive immune response
Authors
Heiner Appel
René Maier
Peihua Wu
Rebecca Scheer
Axel Hempfing
Ralph Kayser
Andreas Thiel
Andreas Radbruch
Christoph Loddenkemper
Joachim Sieper
Publication date
01-06-2011
Publisher
BioMed Central
Published in
Arthritis Research & Therapy / Issue 3/2011
Electronic ISSN: 1478-6362
DOI
https://doi.org/10.1186/ar3370

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