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Arthritis Research & Therapy
Published in: Arthritis Research & Therapy 5/2006

Open Access 01-10-2006 | Research article

Preventing autoimmune arthritis using antigen-specific immature dendritic cells: a novel tolerogenic vaccine

Authors: Igor Popov, Mu Li, Xiufen Zheng, Hongtao San, Xusheng Zhang, Thomas E Ichim, Motohiko Suzuki, Biao Feng, Costin Vladau, Robert Zhong, Bertha Garcia, Gill Strejan, Robert D Inman, Wei-Ping Min

Published in: Arthritis Research & Therapy | Issue 5/2006

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Abstract

Conventional treatments for autoimmune diseases have relied heavily on nonspecific immune suppressants, which possess a variety of adverse effects without inhibiting the autoimmune process in a specific manner. In the present study we demonstrate the effectiveness of antigen-specific, maturation-resistant, tolerogenic dendritic cells (DC) in suppressing collagen-induced arthritis, a murine model of rheumatoid arthritis. Treatment of DC progenitors with the NF-κB inhibiting agent LF 15-0195 (LF) resulted in a population of tolerogenic DC that are characterized by low expression of MHC class II, CD40, and CD86 molecules, as well as by poor allostimulatory capacity in a mixed leukocyte reaction. Administering LF-treated DC pulsed with keyhole limpet hemocyanin antigen to naïve mice resulted hyporesponsiveness specific for this antigen. Furthermore, administration of LF-treated DC to mice with collagen-induced arthritis resulted in an improved clinical score, in an inhibited antigen-specific T-cell response, and in reduced antibody response to the collagen. The efficacy of LF-treated DC in preventing arthritis was substantiated by histological examination, which revealed a significant decrease in inflammatory cell infiltration in the joints. In conclusion, we demonstrate that in vitro-generated antigen-specific immature DC may have important potential as a tolerogenic vaccine for the treatment of autoimmune arthritis.
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Metadata
Title
Preventing autoimmune arthritis using antigen-specific immature dendritic cells: a novel tolerogenic vaccine
Authors
Igor Popov
Mu Li
Xiufen Zheng
Hongtao San
Xusheng Zhang
Thomas E Ichim
Motohiko Suzuki
Biao Feng
Costin Vladau
Robert Zhong
Bertha Garcia
Gill Strejan
Robert D Inman
Wei-Ping Min
Publication date
01-10-2006
Publisher
BioMed Central
Published in
Arthritis Research & Therapy / Issue 5/2006
Electronic ISSN: 1478-6362
DOI
https://doi.org/10.1186/ar2031

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