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European Journal of Medical Research

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01-12-2010 | Research

Absolute count of T and B lymphocyte subsets is decreased in systemic sclerosis

Authors: T Gambichler, C Tigges, B Burkert, S Höxtermann, P Altmeyer, A Kreuter

Published in: European Journal of Medical Research | Issue 1/2010

Abstract

Background

Previous reports on lymphocyte subpopulations in systemic sclerosis (SSc) are conflicting. Therefore, we aimed to investigate the lymphocyte subsets in SSc patients who were not on immunosuppressive therapy.

Methods

Lymphocyte subsets were assessed in the peripheral blood of SSc patients (n = 29) and healthy controls (n = 29) using the four colour flow cytometry method. Correlation studies were also performed in order to assess the relationship between lymphocyte subsets and clinical parameters.

Results

The absolute count of lymphocytes (P = 0.0042), CD3+ (P = 0.0014), CD4+ (P = 0.0070), CD8+ (P = 0.021), and CD19+ cells (P = 0.024) was significantly decreased in SSc patients when compared to healthy controls. CD4+/CD8+ ratio and the absolute count of CD56+ cells observed in SSc patients did not significantly differ from controls (P = 0.165; P = 0.632, respectively). There was no substantial relationship between the lymphocyte subset levels and clinical features (i.e., SSc subtype, autoantibody profiles, organ involvement), except for a significant inverse correlation of CD19+ cells and the modified Rodnan skin score (r = -0.43, P = 0.020).

Conclusion

Our data support previous reports indicating that subsets of T lymphocytes as well as B lymphocytes play a role in the pathogenesis of SSc.

Leroy EC, Black C, Fleischmajer R, Jablonska S, Krieg T, et al.: Scleroderma (systemic sclerosis): classification, subsets and pathogenesis. J Rheumatol 1988, 15: 202–205.PubMed

Melendro EI, Saldate C, Rivero SJ, Alarcon-Segovia D: T-cell subpopulations in the peripheral blood of patients with connective tissue diseases as determined by flow cytometry using monoclonal antibodies. Clin Immunol Immunopathol 1983, 27: 340–347. 10.1016/0090-1229(83)90086-7PubMedCrossRef

Dalkiliç E, Dilek K, Güllülü M, Yavuz M, Karakoç Y, Yurtkuran M, et al.: Lymphocyte phenotypes in systemic sclerosis. Ann Rheum Dis 1999, 58: 719–720.PubMedCentralPubMedCrossRef

Kahan A, Kahan A, Picard F, Menkès CJ, Amor B: Abnormalities of T lymphocyte subsets in systemic sclerosis demonstrated with anti-CD45RA and anti-CD29 monoclonal antibodies. Ann Rheum Dis 1991, 50: 354–358. 10.1136/ard.50.6.354PubMedCentralPubMedCrossRef

Gorla R, Airò P, Malagoli A, Carella G, Prati E, Brugnoni D, Franceschini F, Cattaneo R: CD4+ and CD8+ subsets: naive and memory cells in the peripheral blood of patients with systemic sclerosis. Clin Rheumatol 1994, 13: 83–87. 10.1007/BF02229871PubMedCrossRef

Fiocco U, Rosada M, Cozzi L, Ortolani C, De Silvestro G, Ruffatti A, Cozzi E, Gallo C, Todesco S: Early phenotypic activation of circulating helper memory T cells in scleroderma: correlation with disease activity. Ann Rheum Dis 1993, 52: 272–277. 10.1136/ard.52.4.272PubMedCentralPubMedCrossRef

Ercole LP, Malvezzi M, Boaretti AC, Utiyama SR, Rachid A: Analysis of lymphocyte subpopulations in systemic sclerosis. J Investig Allergol Clin Immunol 2003, 13: 87–93.PubMed

Ingegnoli F, Trabattoni D, Saresella M, Fantini F, Clerici M: Distinct immune profiles characterize patients with diffuse or limited systemic sclerosis. Clin Immunol 2003, 108: 21–28. 10.1016/S1521-6616(03)00062-7PubMedCrossRef

Artlett CM, Cox LA, Ramos RC, Dennis TN, Fortunato RA, Hummers LK, Jimenez SA, Smith JB: Increased microchimeric CD4+ T lymphocytes in peripheral blood from women with systemic sclerosis. Clin Immunol 2002, 103: 303–308. 10.1006/clim.2002.5222PubMedCrossRef

10.

Tsuji-Yamada J, Nakazawa M, Minami M, Sasaki T: Increased frequency of interleukin 4 producing CD4+ and CD8+ cells in peripheral blood from patients with systemic sclerosis. J Rheumatol 2001, 28: 1252–1258.PubMed

11.

Riccieri V, Parisi G, Spadaro A, Scrivo R, Barone F, Moretti T, Bernardini G, Strom R, Taccari E, Valesini G: Reduced circulating natural killer T cells and gamma/delta T cells in patients with systemic sclerosis. J Rheumatol 2005, 32: 283–286.PubMed

12.

Mitsuo A, Morimoto S, Nakiri Y, Suzuki J, Kaneko H, Tokano Y, Tsuda H, Takasaki Y, Hashimoto H: Decreased CD161+CD8+ T cells in the peripheral blood of patients suffering from rheumatic diseases. Rheumatology (Oxford) 2006, 45: 1477–1484. 10.1093/rheumatology/kel119CrossRef

13.

Sato S, Fujimoto M, Hasegawa M, Takehara K: Altered blood B lymphocyte homeostasis in systemic sclerosis: expanded naive B cells and diminished but activated memory B cells. Arthritis Rheum 2004, 50: 1918–1927. 10.1002/art.20274PubMedCrossRef

14.

Balbir-Gurman A, Denton CP, Nichols B, Knight CJ, Nahir AM, et al.: Non-invasive measurement of biomechanical skin properties in systemic sclerosis. Ann Rheum Dis 2002, 61: 237–241. 10.1136/ard.61.3.237PubMedCentralPubMedCrossRef

15.

Majone F, Olivieri S, Cozzi F, Montaldi A, Tonello M, Visentin MS, Ciprian L, Ruffatti A: Increased apoptosis in circulating lymphocyte cultures of anti-RNA polymerase III positive patients with systemic sclerosis. Rheumatol Int 2008, in press.

Title: Absolute count of T and B lymphocyte subsets is decreased in systemic sclerosis
Authors: T Gambichler
C Tigges
B Burkert
S Höxtermann
P Altmeyer
A Kreuter
Publication date: 01-12-2010
Publisher: BioMed Central
Published in: European Journal of Medical Research / Issue 1/2010
Electronic ISSN: 2047-783X
DOI: https://doi.org/10.1186/2047-783X-15-1-44

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Absolute count of T and B lymphocyte subsets is decreased in systemic sclerosis

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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