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Virology Journal

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Open Access 01-12-2009 | Research

Nuclease-resistant double-stranded DNA controls or standards for hepatitis B virus nucleic acid amplification assays

Authors: Shuang Meng, Sien Zhan, Jinming Li

Published in: Virology Journal | Issue 1/2009

Abstract

Background

Identical blood samples tested using different kits can give markedly different hepatitis B virus (HBV) DNA levels, which can cause difficulty in the interpretation of viral load. A universal double-stranded DNA control or standard that can be used in all commercial HBV DNA nucleic acid amplification assay kits is urgently needed. By aligning all HBV genotypes (A-H), we found that the surface antigen gene and precore-core gene regions of HBV are the most conserved regions among the different HBV genotypes. We constructed a chimeric fragment by overlapping extension polymerase chain reaction and obtained a 1,349-bp HBV_C+S fragment. We then packaged the fragment into lambda phages using a traditional lambda phage cloning procedure.

Results

The obtained armored DNA was resistant to DNase I digestion and was stable, noninfectious to humans, and could be easily extracted using commercial kits. More importantly, the armored DNA may be used with all HBV DNA nucleic acid amplification assay kits.

Conclusions

The lambda phage packaging system can be used as an excellent expression platform for armored DNA. The obtained armored DNA possessed all characteristics of an excellent positive control or standard. In addition, this armored DNA is likely to be appropriate for all commercial HBV DNA nucleic acid amplification detection kits. Thus, the constructed armored DNA can probably be used as a universal positive control or standard in HBV DNA assays.

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Title: Nuclease-resistant double-stranded DNA controls or standards for hepatitis B virus nucleic acid amplification assays
Authors: Shuang Meng
Sien Zhan
Jinming Li
Publication date: 01-12-2009
Publisher: BioMed Central
Published in: Virology Journal / Issue 1/2009
Electronic ISSN: 1743-422X
DOI: https://doi.org/10.1186/1743-422X-6-226

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