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Virology Journal
Published in: Virology Journal 1/2013

Open Access 01-12-2013 | Research

Monitoring of adenovirus serotypes in environmental samples by combined PCR and melting point analyses

Authors: Nils Marten Hartmann, Melanie Dartscht, Regine Szewzyk, Hans-Christoph Selinka

Published in: Virology Journal | Issue 1/2013

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Abstract

Background

Human adenoviruses are promising candidates for addressing health risks associated with enteric viruses in environmental waters. Relatively harmless but common, these DNA viruses persist within the population and are generally considered extremely stable, remaining infectious in water for long periods of time. Group-specific or single species detection of human adenoviruses in environmental samples is usually based on polymerase chain reaction assays. Simultaneous identification of specific species or serotypes needs additional processing. Here we present a simple molecular approach for the monitoring of serotypic diversity in the human adenovirus populations in contaminated water sites.

Methods

Diversity patterns of human adenoviruses in environmental samples, collected in an outdoor artificial stream and pond simulation system, were analyzed using a closed tube polymerase chain reaction method with subsequent melting point analysis.

Results

Human adenovirus serotype 41 was the most prominent adenovirus serotype detected in environmental water samples, but melting point analyses indicated the presence of additional adenovirus serotypes.

Conclusions

Based on investigations with spiked and environmental samples, a combination of qPCR and melting point analysis was shown to identify adenovirus serotypes in sewage contaminated water.
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Metadata
Title
Monitoring of adenovirus serotypes in environmental samples by combined PCR and melting point analyses
Authors
Nils Marten Hartmann
Melanie Dartscht
Regine Szewzyk
Hans-Christoph Selinka
Publication date
01-12-2013
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2013
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/1743-422X-10-190

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