Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on medication adherence

LIVE: Thursday 27th June 2024, 18:00-19:30 (CEST)
Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.
ADVANCED SEARCH
Log in
Top
Virology Journal
Published in: Virology Journal 1/2013

Open Access 01-12-2013 | Research

A recombinant pseudotyped lentivirus expressing the envelope glycoprotein of Hantaan virus induced protective immunity in mice

Authors: Lan Yu, Wentao Bai, Xingan Wu, Liang Zhang, Lei Zhang, Puyuan Li, Fang Wang, Ziyu Liu, Fanglin Zhang, Zhikai Xu

Published in: Virology Journal | Issue 1/2013

Login to get access

Abstract

Background

Hantaviruses cause acute hemorrhagic fever with renal syndrome (HFRS). Currently, several types of inactivated HFRS vaccines are widely used, however the limited ability of these immunogen to elicit neutralizing antibodies restricts vaccine efficacy. Development of an effective vaccine to overcome this weakness is must.

Methods

In the present study, a recombinant pseudotyped lentivirus bearing the hantaan virus (HTNV) envelope glycoproteins (GP), rLV-M, was constructed. C57BL/6 mice were immunized with the rLV-M and a series of immunological assays were conducted to determine the immunogenicity of the recombinant pseudotyped lentivirus. The humoral and cell-mediated immune responses induced by rLV-M were compared with those of the inactivated HFRS vaccine.

Results

Indirect immunofluorescence assay (IFA) showed the rLV-M expressed target proteins in HEK-293cells. In mice, the rLV-M efficiently induced GP-specific humoral responses and protection against HTNV infection. Furthermore, the rLV-M induced higher neutralizing antibody titers than the inactivated HFRS vaccine control.

Conclusions

The results indicated the potential of using a pseudotyped lentivirus as a delivery vector for a hantavirus vaccine immunogen.
Appendix
Available only for authorised users
Literature
1.
Battisti AJ, Chu YK, Chipman PR, Kaufmann B, Jonsson CB, Rossmann MG: Structural studies of Hantaan virus. J Virol 2011, 85: 835-841. 10.1128/JVI.01847-10PubMedPubMedCentralCrossRef
2.
Lober C, Anheier B, Lindow S, Klenk HD, Feldmann H: The Hantaan virus glycoprotein precursor is cleaved at the conserved pentapeptide WAASA. Virology 2001, 289: 224-229. 10.1006/viro.2001.1171PubMedCrossRef
3.
4.
5.
Nichol ST, Spiropoulou CF, Morzunov S, Rollin PE, Ksiazek TG, Feldmann H, Sanchez A, Childs J, Zaki S, Peters CJ: Genetic identification of a hantavirus associated with an outbreak of acute respiratory illness. Science 1993, 262: 914-917. 10.1126/science.8235615PubMedCrossRef
6.
MacNeil A, Ksiazek TG, Rollin PE: Hantavirus pulmonary syndrome, United States, 1993–2009. Emerg Infect Dis 2011, 17: 1195-1201. 10.3201/eid1707.101306PubMedPubMedCentralCrossRef
7.
8.
Hjelle B: Vaccines against hantaviruses. Expert Rev Vaccines 2002, 1: 373-384. 10.1586/14760584.1.3.373PubMedCrossRef
9.
Maes P, Clement J, Van Ranst M: Recent approaches in hantavirus vaccine development. Expert Rev Vaccines 2009, 8: 67-76. 10.1586/14760584.8.1.67PubMedCrossRef
10.
Khaiboullina SF, St Jeor SC: Hantavirus immunology. Viral Immunol 2002, 15: 609-625. 10.1089/088282402320914548PubMedCrossRef
11.
Arikawa J, Schmaljohn AL, Dalrymple JM, Schmaljohn CS: Characterization of Hantaan virus envelope glycoprotein antigenic determinants defined by monoclonal antibodies. J Gen Virol 1989,70(Pt 3):615-624.PubMedCrossRef
12.
Schmaljohn CS, Chu YK, Schmaljohn AL, Dalrymple JM: Antigenic subunits of Hantaan virus expressed by baculovirus and vaccinia virus recombinants. J Virol 1990, 64: 3162-3170.PubMedPubMedCentral
13.
Hooper JW, Custer DM, Thompson E, Schmaljohn CS: DNA vaccination with the Hantaan virus M gene protects Hamsters against three of four HFRS hantaviruses and elicits a high-titer neutralizing antibody response in Rhesus monkeys. J Virol 2001, 75: 8469-8477. 10.1128/JVI.75.18.8469-8477.2001PubMedPubMedCentralCrossRef
14.
Spik KW, Badger C, Mathiessen I, Tjelle T, Hooper JW, Schmaljohn C: Mixing of M segment DNA vaccines to Hantaan virus and Puumala virus reduces their immunogenicity in hamsters. Vaccine 2008, 26: 5177-5181. 10.1016/j.vaccine.2008.03.097PubMedCrossRef
15.
Hu B, Tai A, Wang P: Immunization delivered by lentiviral vectors for cancer and infectious diseases. Immunol Rev 2011, 239: 45-61. 10.1111/j.1600-065X.2010.00967.xPubMedPubMedCentralCrossRef
16.
Pincha M, Sundarasetty BS, Stripecke R: Lentiviral vectors for immunization: an inflammatory field. Expert Rev Vaccines 2010, 9: 309-321. 10.1586/erv.10.9PubMedCrossRef
17.
Iglesias MC, Frenkiel MP, Mollier K, Souque P, Despres P, Charneau P: A single immunization with a minute dose of a lentiviral vector-based vaccine is highly effective at eliciting protective humoral immunity against West Nile virus. J Gene Med 2006, 8: 265-274. 10.1002/jgm.837PubMedCrossRef
18.
Cheng L-f, Bai L, Li K, Li P-y, Hu G, Yu L, Wu X-a, Xu Z-k, Zhang F-l: Detection of the specific antigens in the tissues of the C57BL/6 mice infected with Hantavirus. J Trop Med 2011, 11: 481-482.
19.
Cho HW, Howard CR, Lee HW: Review of an inactivated vaccine against hantaviruses. Intervirology 2002, 45: 328-333. 10.1159/000067925PubMedCrossRef
20.
Zhang FL, Wu XA, Luo W, Bai WT, Liu Y, Yan Y, Wang HT, Xu ZK: The expression and genetic immunization of chimeric fragment of Hantaan virus M and S segments. Biochem Biophys Res Commun 2007, 354: 858-863. 10.1016/j.bbrc.2007.01.020PubMedCrossRef
21.
Custer DM, Thompson E, Schmaljohn CS, Ksiazek TG, Hooper JW: Active and passive vaccination against hantavirus pulmonary syndrome with Andes virus M genome segment-based DNA vaccine. J Virol 2003, 77: 9894-9905. 10.1128/JVI.77.18.9894-9905.2003PubMedPubMedCentralCrossRef
22.
Hooper JW, Kamrud KI, Elgh F, Custer D, Schmaljohn CS: DNA vaccination with hantavirus M segment elicits neutralizing antibodies and protects against seoul virus infection. Virology 1999, 255: 269-278. 10.1006/viro.1998.9586PubMedCrossRef
23.
Hooper JW, Ferro AM, Wahl-Jensen V: Immune serum produced by DNA vaccination protects hamsters against lethal respiratory challenge with Andes virus. J Virol 2008, 82: 1332-1338. 10.1128/JVI.01822-07PubMedPubMedCentralCrossRef
24.
Arikawa J, Yao JS, Yoshimatsu K, Takashima I, Hashimoto N: Protective role of antigenic sites on the envelope protein of Hantaan virus defined by monoclonal antibodies. Arch Virol 1992, 126: 271-281. 10.1007/BF01309700PubMedCrossRef
25.
Lawson ND, Stillman EA, Whitt MA, Rose JK: Recombinant vesicular stomatitis viruses from DNA. Proc Natl Acad Sci USA 1995, 92: 4477-4481. 10.1073/pnas.92.10.4477PubMedPubMedCentralCrossRef
26.
Whelan SP, Ball LA, Barr JN, Wertz GT: Efficient recovery of infectious vesicular stomatitis virus entirely from cDNA clones. Proc Natl Acad Sci USA 1995, 92: 8388-8392. 10.1073/pnas.92.18.8388PubMedPubMedCentralCrossRef
27.
Dull T, Zufferey R, Kelly M, Mandel RJ, Nguyen M, Trono D, Naldini L: A third-generation lentivirus vector with a conditional packaging system. J Virol 1998, 72: 8463-8471.PubMedPubMedCentral
28.
Zufferey R, Dull T, Mandel RJ, Bukovsky A, Quiroz D, Naldini L, Trono D: Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery. J Virol 1998, 72: 9873-9880.PubMedPubMedCentral
29.
Trono D: Lentiviral vectors: turning a deadly foe into a therapeutic agent. Gene Ther 2000, 7: 20-23. 10.1038/sj.gt.3301105PubMedCrossRef
30.
Pluta K, Kacprzak MM: Use of HIV as a gene transfer vector. Acta Biochim Pol 2009, 56: 531-595.PubMed
31.
Huret C, Desjardins D, Miyalou M, Levacher B, Amadoudji Zin M, Bonduelle O, Combadiere B, Dalba C, Klatzmann D, Bellier B: Recombinant retrovirus-derived virus-like particle-based vaccines induce hepatitis C virus-specific cellular and neutralizing immune responses in mice. Vaccine 2013, 31: 1540-1547. 10.1016/j.vaccine.2012.05.025PubMedCrossRef
32.
Lee BH, Yoshimatsu K, Araki K, Okumura M, Nakamura I, Arikawa J: A pseudotype vesicular stomatitis virus containing Hantaan virus envelope glycoproteins G1 and G2 as an alternative to hantavirus vaccine in mice. Vaccine 2006, 24: 2928-2934. 10.1016/j.vaccine.2005.12.040PubMedCrossRef
33.
Wang PZ, Huang CX, Zhang Y, Li ZD, Yo HT, Zhang Y, Jia ZS, Wang JP, Lian JQ, Sun YT, Bai XF: Analysis of the immune response to Hantaan virus nucleocapsid protein C-terminal-specific CD8(+) T cells in patients with hemorrhagic fever with renal syndrome. Viral Immunol 2009, 22: 253-260. 10.1089/vim.2008.0097PubMedCrossRef
34.
Wang M, Wang J, Zhu Y, Xu Z, Yang K, Yang A, Jin B: Cellular immune response to Hantaan virus nucleocapsid protein in the acute phase of hemorrhagic fever with renal syndrome: correlation with disease severity. J Infect Dis 2009, 199: 188-195. 10.1086/595834PubMedCrossRef
35.
Woo GJ, Chun EY, Kim KH, Kim W: Analysis of immune responses against nucleocapsid protein of the Hantaan virus elicited by virus infection or DNA vaccination. J Microbiol 2005, 43: 537-545.PubMed
36.
Wang M, Pennock DG, Spik KW, Schmaljohn CS: Epitope mapping studies with neutralizing and non-neutralizing monoclonal antibodies to the G1 and G2 envelope glycoproteins of Hantaan virus. Virology 1993, 197: 757-766. 10.1006/viro.1993.1652PubMedCrossRef
37.
Xu Z, Wei L, Wang L, Wang H, Jiang S: The in vitro and in vivo protective activity of monoclonal antibodies directed against Hantaan virus: potential application for immunotherapy and passive immunization. Biochem Biophys Res Commun 2002, 298: 552-558. 10.1016/S0006-291X(02)02491-9PubMedCrossRef
38.
Liang MF, Song G, Hang CS, Li DX, Qiu HL: Preliminary study on structural protein of epidemic hemorrhagic fever virus using monoclonal antibodies. Chinese Journal of Virology 1989, 5: 217-224.
39.
Yoo DW, Kang CY: Nucleotide sequence of the M segment of the genomic RNA of Hantaan virus 76–118. Nucleic Acids Res 1987, 15: 6299-6300. 10.1093/nar/15.15.6299PubMedPubMedCentralCrossRef
40.
Li PY, Yu L, Wu XA, Bai WT, Li K, Wang HT, Hu G, Zhang L, Zhang FL, Xu ZK: Modification of the adenoviral transfer vector enhances expression of the Hantavirus fusion protein GnS0.7 and induces a strong immune response in C57BL/6 mice. J Virol Methods 2012, 179: 90-96. 10.1016/j.jviromet.2011.10.005PubMedCrossRef
41.
Xu ZK, Zou HS, Wang LY, Wang HT, Xiao Y, Wang MX: Comparison of the micro cell culture test and the plaque assay for Hemorrhagic Fever with Renal Syndrome Virus and its neutralizing antibody. Chin J Exp Clin Virol 1995, 9: 126-128.
42.
Li K, Li PY, Wu XA, Zhang L, Liu ZY, Yu L, Zhang L, Cheng LF, Bai WT, Zhang FL, Xu ZK: Induction of Hantaan virus-specific immune responses in C57BL/6 mice by immunization with a modified recombinant adenovirus containing the chimeric gene, GcS0.7. Int J Mol Med 2013. Epub ahead of print
Metadata
Title
A recombinant pseudotyped lentivirus expressing the envelope glycoprotein of Hantaan virus induced protective immunity in mice
Authors
Lan Yu
Wentao Bai
Xingan Wu
Liang Zhang
Lei Zhang
Puyuan Li
Fang Wang
Ziyu Liu
Fanglin Zhang
Zhikai Xu
Publication date
01-12-2013
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2013
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/1743-422X-10-301

Other articles of this Issue 1/2013

Virology Journal 1/2013 Go to the issue

Research

Antiviral activity of stachyflin on influenza A viruses of different hemagglutinin subtypes

Research

Pepino mosaic virus genotype shift in North America and development of a loop-mediated isothermal amplification for rapid genotype identification

Short report

Interleukin-1β receptor expressed by modified vaccinia virus Ankara interferes with interleukin-1β activity produced in various virus-infected antigen-presenting cells

Research

Distinction of subtype-specific antibodies against European porcine influenza viruses by indirect ELISA based on recombinant hemagglutinin protein fragment-1

Methodology

Use of immuno-dominant epitope derived from genotype 4 as a diagnostic reagent for detecting the antibodies against Hepatitis E Virus

Research

Genomic adaptation of the ISA virus to Salmo salar codon usage
Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Reserve your place

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin
Prof. Florian Limbourg
Prof. Anoop Chauhan
Developed by: Springer Medicine
Obesity Clinical Trial Summary

At a glance: The STEP trials

Read more

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine
Image Credits