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Reproductive Biology and Endocrinology

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Open Access 01-12-2011 | Methodology

Detection of vitellogenin incorporation into zebrafish oocytes by FITC fluorescence

Authors: Yohei Matsuda, Yoshie Ito, Hisashi Hashimoto, Hayato Yokoi, Tohru Suzuki

Published in: Reproductive Biology and Endocrinology | Issue 1/2011

Abstract

Background

Large volumes of lymph can be collected from the eye-sacs of bubble-eye goldfish. We attempted to induce vitellogenin (Vtg) in the eye-sac lymph of bubble-eye goldfish and develop a method for visualizing Vtg incorporation by zebrafish oocytes using FITC-labeling.

Methods

Estrogen efficiently induced Vtg in the eye-sac lymph of goldfish. After FITC-labeled Vtg was prepared, it was injected into mature female zebrafish.

Results

Incorporation of FITC-labeled Vtg by zebrafish oocytes was detected in in vivo and in vitro experiments. The embryos obtained from zebrafish females injected with FITC-labeled Vtg emitted FITC fluorescence from the yolk sac and developed normally.

Conclusion

This method for achieving Vtg incorporation by zebrafish oocytes could be useful in experiments related to the development and endocrinology of zebrafish oocytes.

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Title: Detection of vitellogenin incorporation into zebrafish oocytes by FITC fluorescence
Authors: Yohei Matsuda
Yoshie Ito
Hisashi Hashimoto
Hayato Yokoi
Tohru Suzuki
Publication date: 01-12-2011
Publisher: BioMed Central
Published in: Reproductive Biology and Endocrinology / Issue 1/2011
Electronic ISSN: 1477-7827
DOI: https://doi.org/10.1186/1477-7827-9-45

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Detection of vitellogenin incorporation into zebrafish oocytes by FITC fluorescence

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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