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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2012

Open Access 01-12-2012 | Research article

Improvements of insulin resistance in ovariectomized rats by a novel phytoestrogen from Curcuma comosa Roxb

Authors: Mujalin Prasannarong, Vitoon Saengsirisuwan, Pawinee Piyachaturawat, Apichart Suksamrarn

Published in: BMC Complementary Medicine and Therapies | Issue 1/2012

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Abstract

Background

Curcuma comosa Roxb. (C. comosa) is an indigenous medicinal herb that has been used in Thailand as a dietary supplement to relieve postmenopausal symptoms. Recently, a novel phytoestrogen, (3R)-1,7-diphenyl-(4E,6E)-4,6-heptadien-3-ol or compound 049, has been isolated and no study thus far has investigated the role of C. comosa in preventing metabolic alterations occurring in estrogen-deprived state. The present study investigated the long-term effects (12 weeks) of C. comosa hexane extract and compound 049 on insulin resistance in prolonged estrogen-deprived rats.

Methods

Female Sprague-Dawley rats were ovariectomized (OVX) and treated with C. comosa hexane extract (125 mg, 250 mg, or 500 mg/kg body weight (BW)) and compound 049 (50 mg/kg BW) intraperitoneally three times per week for 12 weeks. Body weight, food intake, visceral fat weight, uterine weight, serum lipid profile, glucose tolerance, insulin action on skeletal muscle glucose transport activity, and GLUT-4 protein expression were determined.

Results

Prolonged ovariectomy resulted in dyslipidemia, impaired glucose tolerance and insulin-stimulated skeletal muscle glucose transport, as compared to SHAM. Treatment with C. comosa hexane extract and compound 049, three times per week for 12 weeks, markedly reduced serum total cholesterol and low-density lipoprotein levels, improved insulin sensitivity and partially restored uterine weights in ovariectomized rats. In addition, compound 049 or high doses of C. comosa hexane extract enhanced insulin-mediated glucose uptake in skeletal muscle and increased muscle GLUT-4 protein levels.

Conclusions

Treatment with C. comosa and its diarylheptanoid derivative improved glucose and lipid metabolism in estrogen-deprived rats, supporting the traditional use of this natural phytoestrogen as a strategy for relieving insulin resistance and its related metabolic defects in postmenopausal women.
Appendix
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Literature
1.
DeFronzo RA, Ferrannini E: Insulin resistance. A multifaceted syndrome responsible for NIDDM, obesity, hypertension, dyslipidemia, and atherosclerotic cardiovascular disease. Diabetes Care. 1991, 14: 173-194. 10.2337/diacare.14.3.173.CrossRefPubMed
2.
Lindheim S, Buchanan TA, Duffy DM, Vijod MA, Kojima T, Stanczyk FZ, Lobo RA: Comparison of estimates of insulin sensitivity in pre- and postmenopausal women using the insulin tolerance test and the frequently sampled intravenous glucose tolerance test. J Soc Gynecol Invest. 1994, 1: 150-154.
3.
Pfeilschifter J, Koditz R, Pfohl M, Schatz H: Changes in proinflammatory cytokine activity after menopause. Endocr Rev. 2002, 23: 90-119. 10.1210/er.23.1.90.CrossRefPubMed
4.
Sites CK, Toth MJ, Cushman M, L'Hommedieu GD, Tchernof A, Tracy RP, Poehlman ET: Menopause-related differences in inflammation markers and their relationship to body fat distribution and insulin-stimulated glucose disposal. Fertil Steril. 2002, 77: 128-135. 10.1016/S0015-0282(01)02934-X.CrossRefPubMed
5.
Carr M: The emergence of the metabolic syndrome with menopause. J Clin Endocrinol Metab. 2003, 88: 2404-2411. 10.1210/jc.2003-030242.CrossRefPubMed
6.
López-Candales A: Metabolic syndrome X: a comprehensive review of the pathophysiology and recommended therapy. J Med. 2001, 32: 283-300.PubMed
7.
Women's Health Initiative Investigators: Effect of oestrogen plus progestin on the incidence of diabetes in postmenopausal women: results from the women's health initiative hormone trial. Diabetologia. 2004, 47: 1175-1187.
8.
Women's Health Initiative Investigators: Risk and benefits of estrogen plus progestin in healthy postmenopausal women. JAMA. 2002, 288: 321-333. 10.1001/jama.288.3.321.CrossRef
9.
Lethaby AE, Brown J, Marjoribanks J, Kronenberg F, Roberts H, Eden J: Phytoestrogens for vasomotor menopausal symptoms. Cochrane Database Syst Rev. 2007, 17: CD001395-
10.
de Kleijn MJJ, van der Schouw YT, Wilson PWF, Grobbee DE, Jacques PF: Dietary intake of phytoestrogens is associated with a favorable metabolic cardiovascular risk profile in postmenopausal U.S. women: the framingham study. J Nutr. 2002, 132: 276-282.PubMed
11.
van der Schouw YT, Pijpe A, Lebrun CEI, Bots ML, Peeters PHM, van Staveren WA, Lamberts SWJ, Grobbee DE: Higher usual dietary intake of phytoestrogens is associated with lower aortic stiffness in postmenopausal women. Arterioscler Thromb Vasc Biol. 2002, 22: 1316-1322. 10.1161/01.ATV.0000027176.83618.1A.CrossRefPubMed
12.
Ingram D, Sanders K, Kolybaba M, Lopez D: Case-control study of phyto-oestrogens and breast cancer. Lancet. 1997, 350: 990-994. 10.1016/S0140-6736(97)01339-1.CrossRefPubMed
13.
Adlercreutz H: Phyto-oestrogens and cancer. Lancet Oncol. 2002, 3: 364-373. 10.1016/S1470-2045(02)00777-5.CrossRefPubMed
14.
Piyachaturawat P, Charoenpiboonsin J, Toskulkao C, Suksamrarn A: Reduction of plasma cholesterol by curcuma comosa extract in hypercholesterolaemic hamsters. J Ethnopharmacol. 1999, 66: 199-204. 10.1016/S0378-8741(98)00170-6.CrossRefPubMed
15.
Piyachaturawat P, Srivoraphan P, Chuncharunee A, Komaratat P, Suksamrarn A: Cholesterol lowering effects of a choleretic phloracetophenone in hypercholesterolemic hamsters. Eur J Pharmacol. 2002, 439: 141-147. 10.1016/S0014-2999(02)01453-X.CrossRefPubMed
16.
Jantaratnotai N, Utaisincharoen P, Piyachaturawat P, Chongthammakun S, Sanvarinda Y: Inhibitory effect of Curcuma comosa on NO production and cytokine expression in LPS-activated microglia. Life Sci. 2005, 78: 571-577.CrossRefPubMed
17.
Sodsai A, Piyachaturawat P, Sophasan S, Suksamrarn A, Vongsakul M: Suppression by Curcuma comosa Roxb. of pro-inflammatory cytokine secretion in phorbol-12-myristate-13-acetate stimulated human mononuclear cells. Int Immunopharmacol. 2007, 7: 524-531. 10.1016/j.intimp.2006.12.013.CrossRefPubMed
18.
Su J, Sripanidkulchai B, Sripanidkulchai K, Piyachaturawat P, Wara-Aswapati N: Effect of Curcuma comosa and estradiol on the spatial memory and hippocampal estrogen receptor in the post-training ovariectomized rats. J Nat Med. 2011, 65: 57-62. 10.1007/s11418-010-0457-y.CrossRefPubMed
19.
Weerachayaphorn J, Chuncharunee A, Mahagita C, Lewchalermwongse B, Suksamrarn A, Piyachaturawat P: A protective effect of curcuma comosa roxb. on bone loss in estrogen deficient mice. J Ethnopharmacol. 2011, 137: 956-962. 10.1016/j.jep.2011.06.040.CrossRefPubMed
20.
Suksamrarn A, Ponglikitmongkol M, Wongkrajang K, Chindaduang A, Kittidanairak S, Jankam A, Yingyongnarongkul B, Kittipanumat N, Chokchaisiri R, Khetkam P, Piyachaturawat P: Diarylheptanoids, new phytoestrogens from the rhizomes of Curcuma comosa: Isolation, chemical modification and estrogenic activity evaluation. Bioorg Med Chem. 2008, 16: 6891-6902. 10.1016/j.bmc.2008.05.051.CrossRefPubMed
21.
Winuthayanon W, Suksen K, Boonchird C, Chuncharunee A, Ponglikitmongkol M, Suksamrarn A, Piyachaturawat P: Estrogenic activity of diarylheptanoids from Curcuma comosa Roxb. requires metabolic activation. J Agric Food Chem. 2009, 57: 840-845. 10.1021/jf802702c.CrossRefPubMed
22.
Soontornchainaksaeng P, Jenjittikul T: Chromosome number variation of phytoestrogen-producing Curcuma (Zingiberaceae) from Thailand. J Nat Med. 2010, 64: 370-377. 10.1007/s11418-010-0414-9.CrossRefPubMed
23.
Cortez MY, Torgan CE, Brozinick JT, Ivy JL: Insulin resistance of obese Zucker rats exercise trained at two different intensities. Am J Physiol. 1991, 261: E613-E619.PubMed
24.
Yki-Jarvinen H, Young AA, Lamkin C, Foley JE: Kinetics of glucose disposal in whole body and across the forearm in man. J Clin Invest. 1987, 79: 1713-1719. 10.1172/JCI113011.CrossRefPubMedPubMedCentral
25.
Henriksen EJ, Halseth AE: Early alterations in soleus GLUT-4, glucose transport, and glycogen in voluntary running rats. J Appl Physiol. 1994, 76: 1862-1867.PubMed
26.
Holloszy JO, Hansen PA: Regulation of glucose transport into skeletal muscle. Rev Physiol Biochem Pharmacol. 1996, 128: 99-193.PubMed
27.
Saengsirisuwan V, Perez FR, Sloniger JA, Maier T, Henriksen EJ: Interactions of exercise training and alpha-lipoic acid on insulin signaling in skeletal muscle of obese Zucker rats. Am J Physiol Endocrinol Metab. 2004, 287: E529-E536. 10.1152/ajpendo.00013.2004.CrossRefPubMed
28.
Park YW, Zhu S, Palaniappan L, Heshka S, Carnethon MR, Heymsfield SB: The metabolic syndrome: prevalence and associated risk factor findings in the US population from the Third National Health and Nutrition Examination Survey, 1988-1994. Arch Intern Med. 2003, 163: 427-436. 10.1001/archinte.163.4.427.CrossRefPubMedPubMedCentral
29.
Sternfeld B, Bhat AK, Wang H, Sharp T, Quesenberry CP: Menopause, physical activity, and body composition/fat distribution in midlife women. Med Sci Sports Exerc. 2005, 37: 1195-1202. 10.1249/01.mss.0000170083.41186.b1.CrossRefPubMed
30.
Bonds DE, Lasser N, Qi L, Brzyski R, Caan B, Heiss G, Limacher MC, Liu JH, Mason E, Oberman A, O'Sullivan MJ, Phillips LS, Prineas RJ, Tinker L: The effect of conjugated equine oestrogen on diabetes incidence: the women's health initiative randomised trial. Diabetologia. 2006, 49: 459-468.CrossRefPubMed
31.
Hodis HN, Mack WJ, Lobo RA, Shoupe D, Sevanian A, Mahrer PR, Selzer RH, Liu C, Azen SP: Estrogen in the prevention of atherosclerosis. Ann Intern Med. 2001, 135: 939-953.CrossRefPubMed
32.
Jones ME, Thorburn AW, Britt KL, Hewitt KN, Wreford NG, Proietto J, Oz OK, Leury BJ, Robertson KM, Yao S, Simpson ER: Aromatase-deficient (ArKO) mice have a phenotype of increased adiposity. Proc Natl Acad Sci USA. 2000, 97: 12735-12740. 10.1073/pnas.97.23.12735.CrossRefPubMedPubMedCentral
33.
Bryzgalova H, Gao H, Ahren B, Zierath JR, Galuska D, Steiler TL, Dahlman-Wright K, Nilsson S, Gustafsson JA, Efendic S, Khan A: Evidence that oestrogen receptor-α plays an important role in the regulation of glucose homeostasis in mice: insulin sensitivity in the liver. Diabetologia. 2006, 49: 588-597. 10.1007/s00125-005-0105-3.CrossRefPubMed
34.
Barros RP, Machado UF, Warner M, Gustafsson JA: Muscle GLUT4 regulation by estrogen receptors ERbeta and ERalpha. Proc Natl Acad Sci USA. 2006, 103: 1605-1608. 10.1073/pnas.0510391103.CrossRefPubMedPubMedCentral
35.
Latour MG, Shinoda M, Lavoie JM: Metabolic effects of physical training in ovariectomized and hyperestrogenic rats. J Appl Physiol. 2001, 90: 235-241.PubMed
36.
Rachoń D, Vortherms T, Seidlová-Wuttke D, Wuttke W: Effects of dietary equol on body weight gain, intra-abdominal fat accumulation, plasma lipids, and glucose tolerance in ovariectomized Sprague-Dawley rats. Menopause. 2007, 14: 1-8. 10.1097/01.gme.0000248705.07333.27.CrossRef
37.
Saengsirisuwan V, Pongseeda S, Prasannarong M, Vichaiwong K, Toskulkao C: Modulation of insulin resistance in ovariectomized rats by exercise training and estrogen replacement. Metabolism. 2009, 58: 38-47. 10.1016/j.metabol.2008.08.004.CrossRefPubMed
38.
Luca C, Olefsky JM: Inflammation and insulin resistance. FEBS Lett. 2008, 582: 97-105. 10.1016/j.febslet.2007.11.057.CrossRefPubMed
39.
Charoenwanthanang P, Lawanprasert S, Phivthong-ngam L, Piyachaturawat P, Sanvarinda Y, Porntadavity S: Effects of Curcuma comosa on the expression of atherosclerosis-related cytokine genes in rabbits fed a high-cholesterol diet. J Ethnopharmacol. 2011, 134: 608-613. 10.1016/j.jep.2011.01.006.CrossRefPubMed
40.
Campos H, McNamara JR, Wilson PW, Ordovas JM, Schaefer EJ: Differences in low density lipoprotein subfractions and apolipoproteins in premenopausal and postmenopausal women. J Clin Endocrinol Metab. 1988, 67: 30-35. 10.1210/jcem-67-1-30.CrossRefPubMed
41.
Jensen J, Nilas L, Christiansen C: Influence of menopause on serum lipid and lipoproteins. Maturitas. 1990, 12: 321-331. 10.1016/0378-5122(90)90012-U.CrossRefPubMed
42.
Piyachaturawat P, Teeratagolpisal N, Toskulkao C, Suksamrarn A: Hypolipidemic effect of Curcuma comosa in mice. Artery. 1997, 22: 233-241.PubMed
43.
Intapad S, Suksamrarn A, Piyachaturawat P: Enhancement of vascular relaxation in rat aorta by phytoestrogens from Curcuma comosa Roxb. Vascul Pharmacol. 2009, 51: 284-290. 10.1016/j.vph.2009.07.005.CrossRefPubMed
44.
Intapad S, Saengsirisuwan V, Prasannarong M, Chuncharunee A, Suvitayawat W, Chokchaisiri R, Suksamrarn A, Piyachaturawat P: Long-term effect of phytoestrogens from Curcuma comosa Roxb. on vascular relaxation in ovariectomized rats. J Agric Food Chem. 2012, 60: 758-764.CrossRefPubMed
45.
Winuthayanon W, Piyachaturawat P, Suksamrarn A, Ponglikitmongkol M, Arao Y, Hewitt SC, Korach KS: Diarylheptanoid phytoestrogens isolated from the medicinal plant Curcuma comosa: biologic actions in vitro and in vivo indicate estrogen receptor-dependent mechanisms. Environ Health Perspect. 2009, 117: 1155-1161.CrossRefPubMedPubMedCentral
Metadata
Title
Improvements of insulin resistance in ovariectomized rats by a novel phytoestrogen from Curcuma comosa Roxb
Authors
Mujalin Prasannarong
Vitoon Saengsirisuwan
Pawinee Piyachaturawat
Apichart Suksamrarn
Publication date
01-12-2012
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2012
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/1472-6882-12-28

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