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BMC Musculoskeletal Disorders

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Open Access 01-12-2013 | Research article

Extracorporeal shockwave therapy shows a number of treatment related chondroprotective effect in osteoarthritis of the knee in rats

Authors: Ching-Jen Wang, Shan-Ling Hsu, Lin-Hsiu Weng, Yi-Chih Sun, Feng-Sheng Wang

Published in: BMC Musculoskeletal Disorders | Issue 1/2013

Abstract

Background

Extracorporeal shockwave therapy (ESWT) shows chondroprotective effect in osteoarthritis of the rat knees. However, the ideal number of ESWT is unknown. This study investigated the effects of different numbers of ESWT in osteoarthritis of the knee in rats.

Methods

Forty-five male Sprague-Dawley rats were divided into five groups. Group I underwent sham arthrotomy without anterior cruciate ligament transection (ACLT) or medial meniscectomy (MM) and received no ESWT. Group II underwent ACLT + MM and received no ESWT. Group III underwent ACLT + MM, and received ESWT once a week for one treatment. Group IV underwent ACLT + MM and received ESWT twice a week for 2 treatments. Group V underwent ACLT + MM and received ESWT three times a week for 3 treatments. Each treatment consisted of 800 impulses of shockwave at 14 Kv to the medial tibia condyle. The evaluations included radiographs of the knee, histomorphological examination and immunohistochemical analysis at 12 weeks.

Results

At 12 weeks, group II and V showed more radiographic arthritis than groups I, III and IV. On histomorphological examination, the Safranin O matrix staining in groups III and IV are significantly better than in groups II and V, and the Mankin scores in groups III and IV are less than groups II and V. Groups III and IV showed significant decreases of Mankin score and increase of Safranin O stain as compared to group I. Group V showed significant increases of Mankin score and a decrease of Safranin O stain as compared to group II. In articular cartilage, group II showed significant increase of MMP13 and decrease of collagen II as compared to group I. Groups III and IV showed significant decrease of MMP13 and increase of collagen II as compared to group I. Group V showed significant increase of MMP13 and decrease of collagen II as compared to group II. In subchondral bone, vWF, VEGF, BMP-2 and osteocalcin significantly decreased in groups II and V, but increased in groups III and IV relative to group I.

Conclusions

ESWT shows a number of treatment related chondroproctective effect in osteoarthritis of the knee in rats.

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The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2474/14/44/prepub

Title: Extracorporeal shockwave therapy shows a number of treatment related chondroprotective effect in osteoarthritis of the knee in rats
Authors: Ching-Jen Wang
Shan-Ling Hsu
Lin-Hsiu Weng
Yi-Chih Sun
Feng-Sheng Wang
Publication date: 01-12-2013
Publisher: BioMed Central
Published in: BMC Musculoskeletal Disorders / Issue 1/2013
Electronic ISSN: 1471-2474
DOI: https://doi.org/10.1186/1471-2474-14-44

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Extracorporeal shockwave therapy shows a number of treatment related chondroprotective effect in osteoarthritis of the knee in rats

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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