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BMC Cancer
Published in: BMC Cancer 1/2007

Open Access 01-12-2007 | Research article

Identification of a robust gene signature that predicts breast cancer outcome in independent data sets

Authors: James E Korkola, Ekaterina Blaveri, Sandy DeVries, Dan H Moore II, E Shelley Hwang, Yunn-Yi Chen, Anne LH Estep, Karen L Chew, Ronald H Jensen, Frederic M Waldman

Published in: BMC Cancer | Issue 1/2007

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Abstract

Background

Breast cancer is a heterogeneous disease, presenting with a wide range of histologic, clinical, and genetic features. Microarray technology has shown promise in predicting outcome in these patients.

Methods

We profiled 162 breast tumors using expression microarrays to stratify tumors based on gene expression. A subset of 55 tumors with extensive follow-up was used to identify gene sets that predicted outcome. The predictive gene set was further tested in previously published data sets.

Results

We used different statistical methods to identify three gene sets associated with disease free survival. A fourth gene set, consisting of 21 genes in common to all three sets, also had the ability to predict patient outcome. To validate the predictive utility of this derived gene set, it was tested in two published data sets from other groups. This gene set resulted in significant separation of patients on the basis of survival in these data sets, correctly predicting outcome in 62–65% of patients. By comparing outcome prediction within subgroups based on ER status, grade, and nodal status, we found that our gene set was most effective in predicting outcome in ER positive and node negative tumors.

Conclusion

This robust gene selection with extensive validation has identified a predictive gene set that may have clinical utility for outcome prediction in breast cancer patients.
Appendix
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Metadata
Title
Identification of a robust gene signature that predicts breast cancer outcome in independent data sets
Authors
James E Korkola
Ekaterina Blaveri
Sandy DeVries
Dan H Moore II
E Shelley Hwang
Yunn-Yi Chen
Anne LH Estep
Karen L Chew
Ronald H Jensen
Frederic M Waldman
Publication date
01-12-2007
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2007
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/1471-2407-7-61

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