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Open Access 01-12-2006 | Research article

Increased mRNA expression levels of ERCC1, OGG1 and RAIin colorectal adenomas and carcinomas

Authors: Mona Sæbø, Camilla Furu Skjelbred, Bjørn Andersen Nexø, Håkan Wallin, Inger-Lise Hansteen, Ulla Vogel, Elin H Kure

Published in: BMC Cancer | Issue 1/2006

Abstract

Background

The majority of colorectal cancer (CRC) cases develop through the adenoma-carcinoma pathway. If an increase in DNA repair expression is detected in both early adenomas and carcinomas it may indicate that low repair capacity in the normal mucosa is a risk factor for adenoma formation.

Methods

We have examined mRNA expression of two DNA repair genes, ERCC1 and OGG1 as well as the putative apoptosis controlling gene RAI, in normal tissues and lesions from 36 cases with adenomas (mild/moderat n = 21 and severe n = 15, dysplasia) and 9 with carcinomas.

Results

Comparing expression levels of ERCC1, OGG1 and RAI between normal tissue and all lesions combined yielded higher expression levels in lesions, 3.3-fold higher (P = 0.005), 5.6-fold higher(P < 3·10^-5) and 7.7-fold higher (P = 0.0005), respectively. The levels of ERCC1, OGG1 and RAI expressions when comparing lesions, did not differ between adenomas and CRC cases, P = 0.836, P = 0.341 and P = 0.909, respectively. When comparing expression levels in normal tissue, the levels for OGG1 and RAI from CRC cases were significantly lower compared to the cases with adenomas, P = 0.012 and P = 0.011, respectively.

Conclusion

Our results suggest that increased expression of defense genes is an early event in the progression of colorectal adenomas to carcinomas.

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The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/6/208/prepub

Title: Increased mRNA expression levels of ERCC1, OGG1 and RAIin colorectal adenomas and carcinomas
Authors: Mona Sæbø
Camilla Furu Skjelbred
Bjørn Andersen Nexø
Håkan Wallin
Inger-Lise Hansteen
Ulla Vogel
Elin H Kure
Publication date: 01-12-2006
Publisher: BioMed Central
Published in: BMC Cancer / Issue 1/2006
Electronic ISSN: 1471-2407
DOI: https://doi.org/10.1186/1471-2407-6-208

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