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BMC Cancer
Published in: BMC Cancer 1/2014

Open Access 01-12-2014 | Research article

Platelet extracts induce growth, migration and invasion in human hepatocellular carcinoma in vitro

Authors: Brian I Carr, Aldo Cavallini, Rosalba D’Alessandro, Maria Grazia Refolo, Catia Lippolis, Antonio Mazzocca, Caterina Messa

Published in: BMC Cancer | Issue 1/2014

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Abstract

Background

Thrombocytopenia has been reported to be associated with small size HCCs, and thrombocytosis to be associated with large size HCCs. The aim was to examine the effects of platelets in relation to HCC cell growth.

Methods

The effects of time-expired pooled normal human platelets were examined on human HCC cell line growth and invasion.

Results

Blood platelet numbers increased with increasing HCC tumor size and portal vein invasion. Platelet extracts enhanced cell growth in 4 human HCC cell lines, as well as cell migration, medium AFP levels and decreased apoptosis. Cell invasion was significantly enhanced, using a Matrigel-coated trans-well membrane and3D (Real-Time Imaging) invasion assay. Western blots showed that platelets caused enhanced phospho-ERK and phospho–JNK signaling and anti-apoptotic effect with increase of Bcl-xL (anti-apoptotic marker) and decrease of Bid (pro-apoptotic marker) levels. Their growth effects were blocked by a JNK inhibitor.

Conclusions

Platelets stimulated growth and invasion of several HCC cell lines in vitro, suggesting that platelets or platelet growth factors could be a potential pharmacological target.
Appendix
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Metadata
Title
Platelet extracts induce growth, migration and invasion in human hepatocellular carcinoma in vitro
Authors
Brian I Carr
Aldo Cavallini
Rosalba D’Alessandro
Maria Grazia Refolo
Catia Lippolis
Antonio Mazzocca
Caterina Messa
Publication date
01-12-2014
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2014
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/1471-2407-14-43

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