Published in:
Open Access 01-12-2003 | Research article
Bioelectronic DNA detection of human papillomaviruses using eSensor™: a model system for detection of multiple pathogens
Published in: BMC Infectious Diseases | Issue 1/2003
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Background
We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor™) in identifying multiple pathogens.
Methods
Two chips were spotted with capture probes consisting of DNA oligonucleotide sequences specific for HPV types. Electrically conductive signal probes were synthesized to be complementary to a distinct region of the amplified HPV target DNA. A portion of the HPV L1 region that was amplified by using consensus primers served as target DNA. The amplified target was mixed with a cocktail of signal probes and added to a cartridge containing a DNA chip to allow for hybridization with complementary capture probes.
Results
Two bioelectric chips were designed and successfully detected 86% of the HPV types contained in clinical samples.
Conclusions
This model system demonstrates the potential of the eSensor platform for rapid and integrated detection of multiple pathogens.