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BMC Immunology
Published in: BMC Immunology 1/2006

Open Access 01-12-2006 | Research article

Human cerebrospinal fluid contains CD4+ memory T cells expressing gut- or skin-specific trafficking determinants: relevance for immunotherapy

Authors: Pia Kivisäkk, Barbara Tucky, Tao Wei, James J Campbell, Richard M Ransohoff

Published in: BMC Immunology | Issue 1/2006

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Abstract

Background

Circulating memory T cells can be divided into tissue-specific subsets, which traffic through distinct tissue compartments during physiologic immune surveillance, based on their expression of adhesion molecules and chemokine receptors. We reasoned that a bias (either enrichment or depletion) of CSF T cell expression of known organ-specific trafficking determinants might suggest that homing of T cells to the subarachnoid space could be governed by a CNS-specific adhesion molecule or chemokine receptor.

Results

The expression of cutaneous leukocyte antigen (CLA) and CC-chemokine receptor 4 (CCR4; associated with skin-homing) as well as the expression of integrin α4β7 and CCR9 (associated with gut-homing) was analyzed on CD4+ memory T cells in CSF from individuals with non-inflammatory neurological diseases using flow cytometry. CSF contained similar proportions of CD4+ memory T cells expressing CLA, CCR4, integrin α4β7 and CCR9 as paired blood samples.

Conclusion

The results extend our previous findings that antigen-experienced CD4+ memory T cells traffic through the CSF in proportion to their abundance in the peripheral circulation. Furthermore, the ready access of skin- and gut-homing CD4+ memory T cells to the CNS compartment via CSF has implications for the mechanisms of action of immunotherapeutic strategies, such as oral tolerance or therapeutic immunization, where immunogens are administered using an oral or subcutaneous route.
Appendix
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Metadata
Title
Human cerebrospinal fluid contains CD4+ memory T cells expressing gut- or skin-specific trafficking determinants: relevance for immunotherapy
Authors
Pia Kivisäkk
Barbara Tucky
Tao Wei
James J Campbell
Richard M Ransohoff
Publication date
01-12-2006
Publisher
BioMed Central
Published in
BMC Immunology / Issue 1/2006
Electronic ISSN: 1471-2172
DOI
https://doi.org/10.1186/1471-2172-7-14

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