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Journal of Diabetes & Metabolic Disorders

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01-06-2021 | Eosinophilia | Research article

Vitamin D3 attenuates oxidative stress and regulates glucose level and leukocyte count in a semi-chronic streptozotocin-induced diabetes model

Authors: Maja Mitrašinović-Brulić, Amela Dervišević, Asija Začiragić, Muhamed Fočak, Amina Valjevac, Almira Hadžović-Džuvo, Damir Suljević

Published in: Journal of Diabetes & Metabolic Disorders | Issue 1/2021

Abstract

Purpose

Vitamin D3 (vit-D3) is a potent immunomodulator with anti-inflammatory and antioxidative properties. We used streptozotocin (STZ)-induced rat model of diabetes (DM) to evaluate the effects of vit-D3. We measured serum biochemical parameters, interleukin-17 (IL-17), osteocalcin (OC), malondialdehyde (MDA), and immune cell count on the 21st day of experiment.

Method

A total of 24 Wistar rats were randomly divided into three groups. Each group had eight rats. During the 1st day of the experiment, the control group was injected intraperitoneally with citrate buffer, while STZ group and STZ + vit-D3 group were injected by a single i.p. dose (35 mg/kg) of STZ dissolved in citrate buffer (pH 4,5; 0,1 M). Vitamin D3 was applied via oral gavage once daily to the STZ + vit-D3 group for a total period of 14 days, starting from the 7th day of the experiment.

Results

STZ rats showed a significant reduction in OC and an increase in MDA and IL-17 serum concentrations compared to the control rats. We also observed a significant STZ-associated decrease in the number of lymphocytes and a significant increase in monocyte and eosinophil number. Oral treatment with vit-D3 to STZ-induced diabetic rats significantly increased OC and decreased MDA serum levels. Furthermore, vit-D3 treatment resulted in a good regulation of hematopoiesis such as increase in the number of segmented granulocytes and lymphocytes and a reduction in the number of monocytes and eosinophils.

Conclusion

Vit-D3 treatment has important therapeutic effects; among many others it can attenuate oxidative stress and ameliorate the hyperglycemic state in the STZ-induced rat diabetic model, which is promising for further clinical trials.

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Title: Vitamin D3 attenuates oxidative stress and regulates glucose level and leukocyte count in a semi-chronic streptozotocin-induced diabetes model
Authors: Maja Mitrašinović-Brulić
Amela Dervišević
Asija Začiragić
Muhamed Fočak
Amina Valjevac
Almira Hadžović-Džuvo
Damir Suljević
Publication date: 01-06-2021
Publisher: Springer International Publishing
Keyword: Eosinophilia
Published in: Journal of Diabetes & Metabolic Disorders / Issue 1/2021
Electronic ISSN: 2251-6581
DOI: https://doi.org/10.1007/s40200-021-00814-2

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Vitamin D3 attenuates oxidative stress and regulates glucose level and leukocyte count in a semi-chronic streptozotocin-induced diabetes model

Abstract

Purpose

Method

Results

Conclusion

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