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International Journal of Diabetes in Developing Countries

Published in:

01-09-2018 | Original Article

SNP in microRNA sequences or binding sites of miRNAs: association with type 2 diabetes mellitus susceptibility and in silico analysis

Authors: Mahdiyeh Moudi, Mostafa Montazer Zohour

Published in: International Journal of Diabetes in Developing Countries | Issue 3/2018

Abstract

Type 2 diabetes mellitus (T2DM) is characterized with defects in insulin secretion, increased hepatic glucose production, and resistance to the action of insulin. The Iranian population is genetically predisposed to T2DM similar to other Asian populations. Therefore, it is imperative to search the genetic variations in T2DM susceptibility unique to the Iranian population. Three hundred patients with type 2 diabetes mellitus and 300 healthy subjects were selected. Peripheral venous blood samples were collected from all subjects. SNPs of mir-605 and the 3′-UTR of ESR1 gene were genotyped by PCR-RFLP. Also, in silico analysis was performed to evaluate potential effects of two SNPs. A significant association was found between all genotypes of ESR1 and T2DM. The data analysis identified an increased risk of T2DM associated with rs9341070 CT genotype among case subjects in relation to control subjects (p value = 0.005; OR = 2.05; 95% CI = 1.24–3.28). The rs2043556 genotypes in miR-605 were significantly correlated with T2DM and there were correlations between GG genotypes of mir-605 rs2043556 with decreased risk for T2DM (p value = 0.01; OR = 0.38; 95% CI = 0.18–0.80). The rs9341070 CT acted as a non-protective factor to increase the risk of T2DM, while the rs2043556 GG genotype is identified as protective factor in diabetes mellitus type 2. According to bioinformatics analysis, it was predicted that rs9341070 in 3′-UTR of ESR1 gene and rs2043556 in mir-605 possibly participate in the regulation of mature miRNA and mRNA targets.

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Title: SNP in microRNA sequences or binding sites of miRNAs: association with type 2 diabetes mellitus susceptibility and in silico analysis
Authors: Mahdiyeh Moudi
Mostafa Montazer Zohour
Publication date: 01-09-2018
Publisher: Springer India
Published in: International Journal of Diabetes in Developing Countries / Issue 3/2018
Print ISSN: 0973-3930
Electronic ISSN: 1998-3832
DOI: https://doi.org/10.1007/s13410-017-0587-9

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