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Published in:

01-06-2014

Hepatitis C virus (HCV) interaction with astrocytes: nonproductive infection and induction of IL-18

Authors: Ziqing Liu, Fang Zhao, Johnny J. He

Published in: Journal of NeuroVirology | Issue 3/2014

Abstract

Hepatitis C virus (HCV) infection causes the central nervous system (CNS) abnormalities in more than 50 % of chronically infected subjects. However, the underlying mechanisms are largely unknown. In this study, we characterized the HCV interactions with astrocytes, one of the putative HCV target cells in the brain. We demonstrated that primary human astrocytes (PHA) were very inefficiently infected by HCV, either in the cell-free form or through cell–cell contact. We then determined the potential restriction steps of HCV infection and replication in these cells. PHA expressed all known HCV receptors but failed to support HCV entry. HCV IRES-mediated RNA translation was functional in PHA and further enhanced by miR122 expression. Nevertheless, PHA did not support HCV replication regardless of miR122 expression. To our great surprise, we found that HCV exposure induced robust IL-18 expression in PHA and exhibited direct neurotoxicity. Taken together, these results showed that astrocytes did not support productive HCV infection and replication, but HCV interactions with astrocytes and neurons alone might be sufficient to cause CNS dysfunction.

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Title: Hepatitis C virus (HCV) interaction with astrocytes: nonproductive infection and induction of IL-18
Authors: Ziqing Liu
Fang Zhao
Johnny J. He
Publication date: 01-06-2014
Publisher: Springer US
Published in: Journal of NeuroVirology / Issue 3/2014
Print ISSN: 1355-0284
Electronic ISSN: 1538-2443
DOI: https://doi.org/10.1007/s13365-014-0245-7

ACC 2024 Congress

Springer Medicine

Hepatitis C virus (HCV) interaction with astrocytes: nonproductive infection and induction of IL-18

Abstract

ACC 2024 Congress

Springer Medicine

Abstract

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