Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on sleep in brain health

LIVE: Thursday 2nd May 2024, 18:00-19:30 (CEST)

Quality sleep is essential for health. But what happens to our brains when sleep patterns are disturbed? Join our experts to explore the interplay between sleep disruption and neurological diseases, and the questions that you need to be asking your patients to help you prevent the harmful effects of sleep deprivation.
ADVANCED SEARCH
Log in
Top
Tumor Biology
Published in: Tumor Biology 2/2016

01-02-2016 | Original Article

iTRAQ-based quantitative proteomic analysis of esophageal squamous cell carcinoma

Authors: Feiyan Deng, Keming Zhou, Qiaoxin Li, Dong Liu, Mengyan Li, Hui Wang, Wei Zhang, Yuqing Ma

Published in: Tumor Biology | Issue 2/2016

Login to get access

Abstract

Esophageal squamous cell carcinoma (ESCC) is one of the most common cancers. In this study, our objective was to identify differentially regulated proteins in ESCC using isobaric tag for relative and absolute quantification (iTRAQ) technique and liquid chromatography–tandem mass spectrometry (LC–MS/MS). We compared the protein expression profiles of ESCC tumor tissues with the corresponding adjacent normal tissue from three patients. It was determined that 72 and 57 unique proteins were significantly up-regulated and down-regulated in all three samples. In addition, there were 431 significantly differentially regulated proteins having at least two biological samples. This subject found some of the differential proteins, such as prolyl 4-hydroxylase subunit alpha-1, prolyl 4-hydroxylase subunit alpha-2, and calponin-2, immunoglobulin superfamily containing leucine-rich repeat protein, and prolyl 3-hydroxylase1, which were few studies about them in ESCC. In order to determine the results, we performed another independent experiment. Our results indicated quantitative proteomics, as a robust discovery tool for the identification, differentially regulated proteins in cancers.
Appendix
Available only for authorised users
Literature
1.
Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. CA Cancer J Clin. 2011;61(2):69–90.CrossRefPubMed
2.
Zhang P, Xi M, Li Q-Q, He L-R, Liu S-L, Zhao L, et al. The modified Glasgow prognostic score is an independent prognostic factor in patients with inoperable thoracic esophageal squamous cell carcinoma undergoing chemoradiotherapy. J Cancer. 2014;5(8):689–95.CrossRefPubMedPubMedCentral
3.
Keszei AP, Alexandra Goldbohm R, Schouten LJ, Jakszyn P, van den Brandt PA. Dietary N-nitroso compounds, endogenous nitrosation, and the risk of esophageal and gastric cancer subtypes in the Netherlands. Am J Clin Nutr. 2013;97(1):135–46.CrossRefPubMed
4.
5.
Guo X, Hao Y, Kamilijiang M, Hasimu A, Yuan J, Wu G, et al. Potential predictive plasma biomarkers for cervical cancer by 2D-DIGE proteomics and ingenuity pathway analysis. Tumour Biol. 2015;36(3):1711–20.CrossRefPubMed
6.
Atrih A, Mudaliar MA, Zakikhani P, Lamont DJ, Huang JT, Bray SE, et al. Quantitative proteomics in resected renal cancer tissue for biomarker discovery and profiling. Br J Cancer. 2014;110(6):1622–33.CrossRefPubMedPubMedCentral
7.
Gan CS, Chong PK, et al. Technical, experimental, and biological variations in isobaric tags for relatives and absolute quantitation (iTRAQ). J Proteome Res. 2007;6(2):821–7.CrossRefPubMed
8.
Gilmore JM, Washburn MP. Advances in shotgun proteomics and the analysis of membrane proteomes. J Proteomics. 2010;73(11):2078–91.CrossRefPubMed
9.
Xiao Z, Li G, Chen Y, Li M, Peng F, et al. Quantitative proteomic analysis of formalin-fixed and paraffin-embedded nasopharyngeal carcinoma using iTRAQ labeling, two-dimensional liquid chromatography and tandem mass spectrometry. J Histochem Cytochem. 2010;58(6):517–27.CrossRefPubMedPubMedCentral
10.
Finn RD, Marshall M, Bateman A. Pfam: visualization of protein–protein interactions in PDB at domain and amino acid resolutions. Bioinformatics. 2005;21(3):410–2.CrossRefPubMed
11.
Saccone G, Louis C, Zhang H, Petrella V, Di Natale M, Perri M, et al. Male-specific phosphorylated SR proteins in adult flies of the Mediterranean fruitfly Ceratitis capitata. BMC Genetics. 2014;15 Suppl 2:S6.CrossRefPubMedPubMedCentral
12.
Xu Q, Lee C. Discovery of novel splice forms and functional analysis of cancer-specific alternative splicing in human expressed sequences. Nucleic Acids Res. 2003;31(19):5635–43.CrossRefPubMedPubMedCentral
13.
14.
Fortes P, Longman D, McCracken S, Ip JY, Poot R, Mattaj IW, et al. Identification and characterization of RED120: a conserved PWI domain protein with links to splicing and 3′-end formation. FEBS Lett. 2007;581:3087–97.CrossRefPubMed
15.
Zhou A, Ou AC, Cho A, Benz Jr EJ, Huang SC. Novel splicing factor RBM25 modulates Bcl-x Pre-mRNA 5′ splice site selection. Mol Cell Biol. 2008;28(19):5924–36.CrossRefPubMedPubMedCentral
16.
Gilkes DM, Saumendra B, Pallavi C, Denis W, Semenza GL. Hypoxia-inducible factor 1 (HIF-1) promotes extracellular matrix remodeling under hypoxic conditions by inducing P4HA1, P4HA2, and PLOD2 expression in fibroblasts. J Biol Chem. 2013;288(15):10819–29.CrossRefPubMedPubMedCentral
17.
Zhang K, Corsa CA, Ponik SM, Prior JL, et al. The collagen receptor discoidin domain receptor 2 stabilizes SNAIL1 to facilitate breast cancer metastasis. Nat Cell Biol. 2013;15(6):677–87.CrossRefPubMedPubMedCentral
18.
19.
Chakravarthi BV, Pathi SS, Goswami MT, et al. The miR-124-prolyl hydroxylase P4HA1-MMP1 axis plays a critical role in prostate cancer progression. Oncotarget. 2014;5(16):6654–69.CrossRefPubMedPubMedCentral
20.
Pan PW, Zhang Q, Bai F, Hou J, Bai G. Profiling and comparative analysis of glycoproteins in Hs578BST and Hs578T and investigation of prolyl 4-hydroxylase alpha polypeptide II expression and influence in breast cancer cells. Biochemistry (Mosc). 2012;77(5):539–45.CrossRef
21.
Jarzab B, Wiench M, Fujarewicz K, Simek K, et al. Gene expression profile of papillary thyroid cancer: sources of variability and diagnostic implications. Cancer Res. 2005;65(4):1587–97.CrossRefPubMed
22.
Chang KP, Yu JS, Chien KY, Lee CW, Liang Y, et al. Identification of PRDX4 and P4HA2 as metastasis associated proteins in oral cavity squamous cell carcinoma by comparative tissue proteomics of microdissected specimens using iTRAQ technology. J Proteome Res. 2011;10(11):4935–47.CrossRefPubMed
23.
Ridley AJ. Rho GTPases and actin dynamics in membrane pro-trusions and vesicle trafficking. Trends Cell Biol. 2006;16(10):522–9.CrossRefPubMed
24.
Kaneko M, Takeoka M, Oguchi M, Koganehira Y, et al. Calpon in hl suppresses tumor growth of src-induce d transformed 3Y1 cells in association with a de crease in angiogenesis. Jpn J Cancer Res. 2002;93(8):935–43.CrossRefPubMed
25.
Yanagisawa Y, Takeoka M, Ehara T, Itano N, Miyagawa S, Taniguchi S. Reduction of calponin H1 expression in human colon cancer blood vessels. Eur J Surg Oncol. 2008;34(5):531–7.CrossRefPubMed
26.
Ogura T, Kobayashi H, Ueoka Y, Okugawa K, Kato K, Hirakawa T, et al. Adenovirus-mediated calponin h1 gene therapy directed against peritoneal dissemination of ovarian cancer: bifunctional therapeutic effects on peritoneal cell layer and cancer cells. Clin Cancer Res. 2006;12(17):5216–23.CrossRefPubMed
27.
Hossain MM, Hwang D-Y, Huang Q-Q, Sasaki Y, Jin J-P. Developmentally regulated expression of calponin isoforms and the effect of h2-calponin on cell proliferation. Am J Physiol. 2003;284(1):156–67.CrossRef
28.
Choi SY, Jang JH, Kim KR. Analysis of differentially expressed genes in human rectal carcinoma using suppression subtractive hybridization. Clin Exp Med. 2011;11(4):219–26.CrossRefPubMed
29.
Hossain MM, Hwang D-Y, Huang Q-Q, Sasaki Y, Jin J-P. Developmentally regulated expression of calponin isoforms and the effect of h2-calponin on cell proliferation. Am J Physiol. 2003;284(1):C156–67.CrossRef
30.
Nagasawa A, Kubota R, Imamura Y, Nagamine K, et al. Cloning of the cDNA for a new member of the immunoglobulin superfamily (ISLR) containing leucine-rich repeat (LRR). Genomics. 1997;44(3):273–9.CrossRefPubMed
31.
D’Andrea LD, Regan L. TPR proteins: the versatile helix. Trends Biochem Sci. 2003;28(12):655–62.CrossRefPubMed
32.
Ishikawa Y, Wirz J, Vranka JA, Nagata K, Bächinger HP. Biochemical characterization of the prolyl 3-hydroxylase 1.cartilage-associated protein.cyclophilin B complex. J Biol Chem. 2009;284(26):17641–7.CrossRefPubMedPubMedCentral
33.
Ahmad Mir S, Pavithra R, Jain AP, Khan AA, Datta KK, Mohan SV, et al. LC–MS-based serum metabolomic analysis reveals dysregulation of phosphatidylcholines in esophageal squamous cell carcinoma. J Proteomics. 2015;S1874-3919(15):00231–6.
34.
Li C, Guo X, Jianqing Z, Yang M, Ge B, Li Z. Serum differential protein identification of Xinjiang Kazakh esophageal cancer patients based on the two-dimensional liquid-phase chromatography and LTQ MS. Mol Biol Rep. 2014;41(5):2893–905.CrossRefPubMed
Metadata
Title
iTRAQ-based quantitative proteomic analysis of esophageal squamous cell carcinoma
Authors
Feiyan Deng
Keming Zhou
Qiaoxin Li
Dong Liu
Mengyan Li
Hui Wang
Wei Zhang
Yuqing Ma
Publication date
01-02-2016
Publisher
Springer Netherlands
Published in
Tumor Biology / Issue 2/2016
Print ISSN: 1010-4283
Electronic ISSN: 1423-0380
DOI
https://doi.org/10.1007/s13277-015-3840-1

Other articles of this Issue 2/2016

Tumor Biology 2/2016 Go to the issue

Review

Serum microRNAs as potential noninvasive biomarkers for glioma

Original Article

Synergistic efficacy of γ-radiation together with gallium trichloride and/or doxorubicin against Ehrlich carcinoma in female mice

Original Article

CpG methylation of ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) and P53 mutation pattern in sporadic colorectal cancer

Original Article

Reversal of efflux of an anticancer drug in human drug-resistant breast cancer cells by inhibition of protein kinase Cα (PKCα) activity

Original Article

Combined analysis identifies six genes correlated with augmented malignancy from non-small cell to small cell lung cancer

Original Article

The clinical and biological significance of MICA in clear cell renal cell carcinoma patients
Webinar | 19-02-2024 | 17:30 (CET)

Keynote webinar | Spotlight on antibody–drug conjugates in cancer

Watch now

Antibody–drug conjugates (ADCs) are novel agents that have shown promise across multiple tumor types. Explore the current landscape of ADCs in breast and lung cancer with our experts, and gain insights into the mechanism of action, key clinical trials data, existing challenges, and future directions.

Dr. Véronique Diéras
Prof. Fabrice Barlesi
Developed by: Springer Medicine
Image Credits