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Tumor Biology
Published in: Tumor Biology 2/2015

01-02-2015 | Research Article

WWP1 as a potential tumor oncogene regulates PTEN-Akt signaling pathway in human gastric carcinoma

Authors: Li Zhang, Zongyin Wu, Zhao Ma, Hongtao Liu, Yahong Wu, Qinxian Zhang

Published in: Tumor Biology | Issue 2/2015

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Abstract

Whelming evidence has demonstrated that WW domain containing E3 ubiquitin protein ligase 1 (WWP1) participates in a wide variety of biological processes and is tightly related to the initiation and progression of many tumors. Currently, although mounting evidence supports a role of WWP1 in tumor promotion and tumorigenesis, the potential roles of WWP1 and its biological functions in gastric carcinoma are not fully understood. Here, we found that WWP1 messenger RNA (mRNA) and protein were highly expressed in gastric carcinoma tissues and cells. High WWP1 mRNA and protein levels were tightly related to differentiation status, TNM stage, invasive depth, lymph node metastasis, and poor prognosis in gastric carcinoma. Furthermore, WWP1 siRNA significantly decreased WWP1 protein level in MKN-45 and AGS cells; meanwhile, WWP1 depletion markedly inhibited tumor proliferation in vitro and in vivo, arrested cell cycle at G0/G1 phase, and induced cell apoptosis in MKN-45 and AGS cells. Most notably, WWP1 downregulation both inactivated PTEN-Akt signaling pathway in MKN-45 and AGS cells. Taken altogether, our findings suggest that WWP1 acts as an oncogenic factor and should be considered as a novel interfering molecular target for gastric carcinoma.
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Metadata
Title
WWP1 as a potential tumor oncogene regulates PTEN-Akt signaling pathway in human gastric carcinoma
Authors
Li Zhang
Zongyin Wu
Zhao Ma
Hongtao Liu
Yahong Wu
Qinxian Zhang
Publication date
01-02-2015
Publisher
Springer Netherlands
Published in
Tumor Biology / Issue 2/2015
Print ISSN: 1010-4283
Electronic ISSN: 1423-0380
DOI
https://doi.org/10.1007/s13277-014-2696-0

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