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Published in: Tumor Biology 5/2012

01-10-2012 | Research Article

MicroRNA-181a promotes gastric cancer by negatively regulating tumor suppressor KLF6

Authors: Xiangyang Zhang, Yuqiang Nie, Yanlei Du, Jie Cao, Bo Shen, Yuyuang Li

Published in: Tumor Biology | Issue 5/2012

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Abstract

MicroRNAs have emerged as crucial regulators of tumorigenesis. However, it remains unknown whether miR-181a is involved in the pathogenesis of gastric cancer. In this study, we found that miR-181a is overexpressed in human gastric cancer tissues. Ectopic expression of miR-181a mimic promoted the proliferation, colony formation, migration, and invasion and inhibited the apoptosis of SGC-7901 gastric cancer cells, whereas ectopic expression of miR-181a inhibitor inhibited the malignant phenotypes of SGC-7901 cells. Site-directed mutagenesis and luciferase reporter assay demonstrated that miR-181a repressed KLF6 expression by targeting its 3′-UTR. Western blot analysis further showed that KLF6 protein was significantly decreased or increased when miR-181a mimic or inhibitor was transfected into SGC-7901 cells, respectively. In summary, these data suggest that KLF6 gene is a direct target of miR-181a and miR-181a functions as an oncomir in gastric cancer by repressing the expression of tumor suppressor KLF6.
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Metadata
Title
MicroRNA-181a promotes gastric cancer by negatively regulating tumor suppressor KLF6
Authors
Xiangyang Zhang
Yuqiang Nie
Yanlei Du
Jie Cao
Bo Shen
Yuyuang Li
Publication date
01-10-2012
Publisher
Springer Netherlands
Published in
Tumor Biology / Issue 5/2012
Print ISSN: 1010-4283
Electronic ISSN: 1423-0380
DOI
https://doi.org/10.1007/s13277-012-0414-3

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