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Translational Stroke Research
Published in: Translational Stroke Research 3/2017

01-06-2017 | Original Article

MRI Characterization in the Acute Phase of Experimental Subarachnoid Hemorrhage

Authors: Dewei Guo, D. Andrew Wilkinson, B. Gregory Thompson, Aditya S. Pandey, Richard F Keep, Guohua Xi, Ya Hua

Published in: Translational Stroke Research | Issue 3/2017

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Abstract

A number of mechanisms have been proposed for the early brain injury after subarachnoid hemorrhage (SAH). In this study, we investigated the radiographic characteristics and influence of gender on early brain injury after experimental SAH. SAH was induced by endovascular perforation in male and female rats. Magnetic resonance imaging was performed in a 7.0-T Varian MR scanner at 24 h after SAH. The occurrence and size of T2 lesions, ventricular dilation, and white matter injury (WMI) were determined on T2-weighted images (T2WI). The effects of SAH on heme oxygenase-1 and fibrin/fibrinogen were examined by Western blotting and immunohistochemistry. SAH severity was assessed using a MRI grading system, and neurological function was evaluated according to a modified Garcia’s scoring system. T2 hyperintensity areas and enlarged ventricles were observed in T2WI coronal sections 24 h after SAH. The overall incidence of T2 lesions, WMI, and hydrocephalus was 54, 20, and 63%, respectively. Female rats had a higher incidence of T2 hyperintensity lesions and hydrocephalus, as well as larger T2 lesion volumes and higher average ventricular volume. SAH rats graded at 3–4 (our previously validated MRI grading scale) had larger T2 lesion volumes, more hydrocephalus, and worse neurological function compared with those graded at 0–2. In conclusion, T2 lesion, WMI, and hydrocephalus were the most prevalent MRI characteristics 24 h after experimental SAH. The T2 lesion area matched with fibrinogen/fibrin positive staining in the acute phase of SAH. SAH induced more severe brain injury in females compared to males in the acute phase of SAH.
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Metadata
Title
MRI Characterization in the Acute Phase of Experimental Subarachnoid Hemorrhage
Authors
Dewei Guo
D. Andrew Wilkinson
B. Gregory Thompson
Aditya S. Pandey
Richard F Keep
Guohua Xi
Ya Hua
Publication date
01-06-2017
Publisher
Springer US
Published in
Translational Stroke Research / Issue 3/2017
Print ISSN: 1868-4483
Electronic ISSN: 1868-601X
DOI
https://doi.org/10.1007/s12975-016-0511-5

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