Skip to main content
Top
Published in: Journal of Parasitic Diseases 1/2017

01-03-2017 | Original Article

RETRACTED ARTICLE: Antigen based detection of cystic echinococcosis in buffaloes using ELISA and Dot-EIA

Authors: A. Sangaran, S. T. Bino Sundar, Bhaskaran Ravi Latha

Published in: Journal of Parasitic Diseases | Issue 1/2017

Login to get access

Abstract

Cystic echinococcosis is caused by the larval stage of the dog tapeworm, Echinococcus granulosus. The disease is recognized as one of the world’s major zoonoses affecting human beings and domestic animals apart from its economic and public health importance. Development of the cysts in the intermediate host such as buffaloes occurs in the lungs, liver and other organs. In this study, detection of circulating antigen in the diagnosis of cystic echinococcosis in buffaloes was done using enzyme linked immunosorbent assay and Dot-Enzyme immunoassay (Dot-EIA). The sensitivity and specificity were determined as 89 and 92 % respectively, whereas those of Dot-EIA were determined as 94 and 96 %.
Literature
go back to reference Al Yaman FM, Assaf L, Hailat N, Abdel Hafez FH (1985) Prevalence of hydatidosis in slaughtered animals from North Jordan. Ann Trop Med Parasitol 79:501–506CrossRef Al Yaman FM, Assaf L, Hailat N, Abdel Hafez FH (1985) Prevalence of hydatidosis in slaughtered animals from North Jordan. Ann Trop Med Parasitol 79:501–506CrossRef
go back to reference Craig PS, Nelson GS (1984) The detection of circulating antigen in human hydatid disease. Ann Trop Med Parasitol 78:219–227CrossRef Craig PS, Nelson GS (1984) The detection of circulating antigen in human hydatid disease. Ann Trop Med Parasitol 78:219–227CrossRef
go back to reference Craig PS, Rickard MD (1981) Studies on the specific immunodiagnosis of larval cestode infections of cattle and sheep using antigens purified by affinity chromatography in an enzyme linked immunosorbent assay (ELISA). Int J Parasitol 11:441–449CrossRef Craig PS, Rickard MD (1981) Studies on the specific immunodiagnosis of larval cestode infections of cattle and sheep using antigens purified by affinity chromatography in an enzyme linked immunosorbent assay (ELISA). Int J Parasitol 11:441–449CrossRef
go back to reference El-Muhtaseb HH (1984) Surgical management of hydatid cysts of the liver: retrospective study of 75 cases. Jordan Med J 18:35–46 El-Muhtaseb HH (1984) Surgical management of hydatid cysts of the liver: retrospective study of 75 cases. Jordan Med J 18:35–46
go back to reference Gatne ML, Narasapur VS, Deshpande VS, Niphadkar SM (1990) Protein content and electrophoretic patterns of hydatid fluid. Indian Vet J 67:169–170 Gatne ML, Narasapur VS, Deshpande VS, Niphadkar SM (1990) Protein content and electrophoretic patterns of hydatid fluid. Indian Vet J 67:169–170
go back to reference Judson DG, Dixon JB, Clarkson MJ, Pritchard J (1985) Ovine hydatidosis: some immunological characteristics of the seronegative host. Parasitology 91:349–357CrossRef Judson DG, Dixon JB, Clarkson MJ, Pritchard J (1985) Ovine hydatidosis: some immunological characteristics of the seronegative host. Parasitology 91:349–357CrossRef
go back to reference Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein determination using Folin–Ciocalteu reagent. J Biol Chem 193:265–275PubMed Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein determination using Folin–Ciocalteu reagent. J Biol Chem 193:265–275PubMed
go back to reference Moosa RA, Abdel Hafez SK (1994) Serodiagnosis and sero epidemiology of human unilocular hydatidosis in Jordan. Parasitol Res 80:664–671CrossRef Moosa RA, Abdel Hafez SK (1994) Serodiagnosis and sero epidemiology of human unilocular hydatidosis in Jordan. Parasitol Res 80:664–671CrossRef
go back to reference Romia SA, Yousuf ME, Handoussa AE, Rizk HM, Sallam SM (1992) Dot-ELISA as a diagnostic test in hydatid disease. J Egypt Soc Parasitol 22:603–610PubMed Romia SA, Yousuf ME, Handoussa AE, Rizk HM, Sallam SM (1992) Dot-ELISA as a diagnostic test in hydatid disease. J Egypt Soc Parasitol 22:603–610PubMed
go back to reference Schantz PM (1972) Hydatidosis: magnitude del problemyt perspectivase de control. Biol Sanit-Panam 74:187–197 [Schwabe, C.W.1986. Current issues of hydatid disease: a zoonosis of increasing importance in R.C.A. Thompson] The Biology of Echinococcus and hydatid disease. George Allen and Unwin, London, pp. 81–113. Schantz PM (1972) Hydatidosis: magnitude del problemyt perspectivase de control. Biol Sanit-Panam 74:187–197 [Schwabe, C.W.1986. Current issues of hydatid disease: a zoonosis of increasing importance in R.C.A. Thompson] The Biology of Echinococcus and hydatid disease. George Allen and Unwin, London, pp. 81–113.
Metadata
Title
RETRACTED ARTICLE: Antigen based detection of cystic echinococcosis in buffaloes using ELISA and Dot-EIA
Authors
A. Sangaran
S. T. Bino Sundar
Bhaskaran Ravi Latha
Publication date
01-03-2017
Publisher
Springer India
Published in
Journal of Parasitic Diseases / Issue 1/2017
Print ISSN: 0971-7196
Electronic ISSN: 0975-0703
DOI
https://doi.org/10.1007/s12639-016-0762-9

Other articles of this Issue 1/2017

Journal of Parasitic Diseases 1/2017 Go to the issue