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01-09-2016 | Original Article

In vitro production of Toxocara canis excretory-secretory (TES) antigen

Authors: Divyamol Thomas, N. Jeyathilakan, S. Abdul Basith, T. M. A. Senthilkumar

Published in: Journal of Parasitic Diseases | Issue 3/2016

Abstract

Toxocara canis is a widespread gastrointestinal nematode parasite of dogs and cause Toxocara larva migrans, an important zoonotic disease in humans on ingestion of infective eggs. Toxocarosis is one of the few human parasitic diseases whose serodiagnosis uses a standardized antigen, T. canis excretory secretory antigen (TES). The present study describes collection of T. canis adult worm, collection and embryonation of T. canis eggs, hatching and separation of T. canis larvae, in vitro maintenance of T. canis second stage larvae for production of TES, concentration of culture fluid TES and yield of TES in correlation with various methods cited in literature.

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Title: In vitro production of Toxocara canis excretory-secretory (TES) antigen
Authors: Divyamol Thomas
N. Jeyathilakan
S. Abdul Basith
T. M. A. Senthilkumar
Publication date: 01-09-2016
Publisher: Springer India
Published in: Journal of Parasitic Diseases / Issue 3/2016
Print ISSN: 0971-7196
Electronic ISSN: 0975-0703
DOI: https://doi.org/10.1007/s12639-014-0630-4

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