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Published in: Journal of Parasitic Diseases 3/2016

01-09-2016 | Original Article

A simple, efficient and inexpensive method for malaria parasites’ DNA catching from fixed Giemsa-stained blood slides

Authors: Abbas Ali Eskandarian, Sara Moradi, Saeed Abedi

Published in: Journal of Parasitic Diseases | Issue 3/2016

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Abstract

As parasitological or microscopic method is the gold standard and the best method for diagnosis of malaria, so fixed Geimsa-stained blood slides in the form of thick and thin blood smears are the most important data collections of malaria, especially historical slides. The parasites are dead but their DNA is valuable for many molecular biologic researches. A simple and efficient method for catching and extraction malaria parasites' DNA with a desired yield from dried and stained blood on slides is the first and major step. Introduction of an applicable, efficient and inexpensive DNA catching method and assessment of its performance in following molecular applications  was the main objective of present study.
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Metadata
Title
A simple, efficient and inexpensive method for malaria parasites’ DNA catching from fixed Giemsa-stained blood slides
Authors
Abbas Ali Eskandarian
Sara Moradi
Saeed Abedi
Publication date
01-09-2016
Publisher
Springer India
Published in
Journal of Parasitic Diseases / Issue 3/2016
Print ISSN: 0971-7196
Electronic ISSN: 0975-0703
DOI
https://doi.org/10.1007/s12639-014-0587-3

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