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01-06-2016 | Original Article

Lactate dehydrogenase (LD), alkaline phosphatase (ALP) isoenzymatic patterns in Iraqi children with visceral leishmaniasis before and after treatment with stibogluconate

Authors: Jasim Hameed Taher, Yassir Mustafa Kamal AL-Mulla Hummadi, Nada Muhammed Taha AL-Bashir, Ali Shaalan AL-Araji

Published in: Journal of Parasitic Diseases | Issue 2/2016

Abstract

The mean levels of alkaline phosphatase (ALP), lactate dehydrogenase enzymes exhibited a significant elevation in visceral leishmaniasis (VL) patients compared to the control. There was no significant change in relation to the sex and age. ALP isoenzymes revealed three banding patterns which differ from the three zymodems which were obtained from control group. These differences may be due to isoenzymes activity of patients with VL before and after therapy. Lactate dehydrogenase (LD) isoenzymes revealed five banding patterns differ from the five normal zymodems. These differences mainly occurred due to LD isoenzymes activity in patients with VL before and after therapy.

Akrawi BA (1985) Purification of LDH-1 and LD-5 isoenzymes from kala-azaric sera and studies of their chemical and physical properties. M.Sc. Thesis, College of Science, Baghdad University

Al-Saffar NR, Al-Mudhaffer SA (1979) Lactate dehydrogenase and other enzymes in kala-azar patients in Iraq. Indian J Med Res 70:598–608PubMed

Alacam T, Gorgul G, Omurlu H (1996) Lactate dehydrogenase activity in periodontal ligament cells stored in different transport media. Oral Surg Oral Med Oral Pathol Oral Radiol 82:321–323CrossRef

Alvar J, Vélez ID, Bern C, Herrero M, Desjeux P, Cano J, Jannin J, den Boer M (2012) WHO Leishmaniasis Control Team Leishmaniasis worldwide and global estimates of its incidence. PLoSOne 7(5):e35671. doi:10.1371/journal.pone.0035671 CrossRef

Benyon S (1998) Mosby Crash Course Metabolism and Nutrition, 1st edn. Mosby Co., London, pp 141–146

Bishope ML, Duben-Engelkirk JL, Fody EP (2000) Clinical chemistry, 4th edn. Lippincott Williams and Wilkins, Philadelphia 185

Bollinger ME, Arredondo-Vega FX, Santistebane I, Schwarz K, Hershfied MS, Lederman HM (1996) Brief report: hepatic dysfunction as a complication of adenosine deaminase deficiency. New Jersey Med 334:1367–1371CrossRef

Broyles DL, Nielsen RG, Busset EM, Lu WD, Mizrahi IA, Nunnelly PA, Ngo TA, Noell J, Chritenson RH, Kress BC (1998) Analytical and Clinical Performance Chararacteristic of Tandem-MP Ostase a new immunoassay for serum bone alkaline phosphatase. Clin Chem 44:2139–2147PubMed

Bruns DE, Emerson JC, Intemann S (1981) Lactate dehydrogenase isoenzymes: changes during the first day after acute myocardial infarction. Clin Chem 27:1821PubMed

Juma ASM, AL-Jeboori TI (1999) Cytogenetic, Biochemical and Bacteriological studies patients infected with Schistosoma haematobium and those with bladder carcinoma. J Fac Med Baghdad 43:453–461

Kreisbergs RA (1978) Diabetic ketoacidosis: new concepts and trends in pathogenesis and treatment. Ann Intern Med 88:681–695CrossRef

Lee TH, Goldman LC (1986) Serum enzyme assays in diagnosis of acute myocardial infarction. Ann Inter Med 105:221–223CrossRef

Lott JA, Nemensanszky E (1987) Lactate dehydrogenase. In: Lott JA, Wolf PL (eds) Clinical enzymology, a case oriented approach. Field, Rich and Associated Inc, New York, pp 213–244

Mackawa M, Kanno T (1989) Laboratory and clinical features of Lactate dehydrogenase subunit deficiencies. Clin Chem Acta 185:299–308CrossRef

Marshal WJ (2000) Isoenzymes. In: Norbert W (ed) Clinical Chemistry, 4th edn. W.B. Saunders Company, Philadelphia, pp 215–299

Martin DW (1981) Blood plasma and clotting. In: Jr Martin DW, Mayes PA, Rodwell VW (eds) Harpers review of biochemistry, 18th edn. Lange Medical Publications, California, pp 540–552

Mathur P, Samantaray JC, Samanta P (2008) High prevalence of functional liver derangement in visceral leishmaniasis at an Indian tertiary care center. Clin Gastroenterol Hepatol 6(10):1170–1172. doi:10.1016/j.cgh CrossRefPubMed

McComb RB, Bowers GN, Poson S (1979) Alkaline phosphatase. Plenum press, New YorkCrossRef

McComb EB (1983) The measurement of LDH. In: Homburger HA, Skokie IL (eds) Clinical and Analytic Concepts in Enzymology, College of American Pathologist, pp 157–71

Meckenzie D, Henderson A (1983) Electrophoresis of Lactate dehydrogenase isoenzymes. Clin Chem 29:189–195

Mercer DW (1978) Improved column method for separating LD isoenzymes. Clin Chem 24:480–482PubMed

Moss DW, Henderson AR (1999) Clinical enzymology. Teitz textbook of clinical chemistry, burtis, CA and Ashwood ER, 3rd edn. W. B. Saunders Co., London, pp 617–716

Moss DW, Henderson AR, Kachmar JF (1986) Enzymes. In: Tietz NW (ed) Tietz Textbook of Clinical Chemistry, 2nd edn. W. B. Saunders Co., London, pp 619–677

Norbort W, Tietz N (1988) Fundamentals of clinical chemistry, 2nd edn. W. B Saunder, Philadelphia, p 61

Pagana KD, Pagana T (1998) Mosby’s manual of diagnostic and laboratory tests. C.V. Mosby Co., St. Louis Inc

Paz JM, Garcia A, Gonzales M (1990) Evaluation and determination of LD - isoenzymes, by chemical inhibition with percholate or with 1, 6-hexamedial. Clin Chem 36:355–358PubMed

Pincus MR, Schaffner JA (1996) Assessment of liver function. In: Henry JB (ed) Clinical diagnosis and management by laboratory methods, 19th edn. W. B. Saunder Co., Philadelphia, pp 253–267

Posen S, Doherty E (1981) The measurement of serum alkaline phosphatase in clinical medicine. Adv Clin Chem 22:165

Prass KW, Fayer R (1981) Hematology of experimental acute Sarcocystis bovicanis infection in calves. Vet Pathol 18:358–367

Rassam MB, AL-Mudhaffar SA (1979) The primary isolation of Leishmania donovani from Iraq on different culture media. Ann Trop Med Parasitol 73(4):345–347CrossRefPubMed

Rassam MB, AL-Shammah GA, AL-Sammerai FT, Salihi AR, AL-Ubaidi MA, AL-Bashir NM, Waheed IN (1996) Serum ceruloplasmin and heat-stable Alklaine phosphatase in sequamous cells carcinoma of the head and neck. J Fac Med 38:280–283

Raymond F, Datta H, Moss D (1991) Alkaline phosphatase isoforms in bile and serum and their generation from cells in vitro. Biochem Biophys Acta 1074:217–222CrossRefPubMed

Rodwell VW (1981) General properties of enzymes. In: Martin DW, Mayes PA, Rodwell VW (eds) Harpers Review of Biochemistry, 18th edn. Lang Medical publications, California, pp 51–63

Rotenberg Z, Weinberg I, Sagie A (1988) Total lactate dehydrogenase and it’s isoenzymes in serum of patients with non-small cell lung cancer. Clin Chem 34:668–670PubMed

Sacks JJ, Delgado DG, Lobel HO, Parker RL (1983) Toxoplasmosis infection associated with eating undercooked venison. Am J Epidemiol 118:832–838PubMed

Schwartz MK (1982) Enzymes tests in cancer. Clin Lab Med 2:479–491PubMed

Shibano M, Macli T, Nishimori Y, Nakamoto I, Ueda E, Masuhara K, Kitani T (1999) Assessment of alkaline phosphatase on the surface membrane of neutrophils by Immunofluorescence. Am J Hematol 60:12–18CrossRefPubMed

Smith AF, Beckett GJ, Walker SW, Rae PW ((2000)) Lecture Notes on Clinical Bochemistry, 6th edn edn. Blackwell Science, Oxford, pp 82–83

van Griensven J, Diro E (2012) Visceral leishmaniasis. Infect Dis Clin North Am 26(2):309–322. doi:10.1016/j.idc.2012.03.005 CrossRefPubMed

Varley H, Gowenlock A, Bell M (1980) Practical Clinical Biochemistry, 5th edn. William Heineman Medical Books Ltd., London

Warren BM (1981) The isoenzyme of alkaline phosphatase. Helena Laboratories, Beaumont

Yousif SI (1981) Physical and chemical properties of alkaline phosphatase isoenzymes isolated from sera of patients with kala-azar. M. Sc. Thesis. College of Science, Baghdad University

Zeltzer PM, Mavangos PJ, Evans AE, Schneider SL (1986) Serum neuron-specific enolase in children with neuroblastoma. Cancer 56:1230–1234CrossRef

Title: Lactate dehydrogenase (LD), alkaline phosphatase (ALP) isoenzymatic patterns in Iraqi children with visceral leishmaniasis before and after treatment with stibogluconate
Authors: Jasim Hameed Taher
Yassir Mustafa Kamal AL-Mulla Hummadi
Nada Muhammed Taha AL-Bashir
Ali Shaalan AL-Araji
Publication date: 01-06-2016
Publisher: Springer India
Published in: Journal of Parasitic Diseases / Issue 2/2016
Print ISSN: 0971-7196
Electronic ISSN: 0975-0703
DOI: https://doi.org/10.1007/s12639-014-0494-7

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Abstract

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