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Reproductive Medicine and Biology
Published in: Reproductive Medicine and Biology 1/2014

01-01-2014 | Original Article

Evaluation of oxygen consumption in human vitrified and warmed pre-antral follicles after prolonged low temperatures

Authors: Toshihiko Kyoya, Yusuke Nakamura, Shizue Miyatani, Tomoko Miyagawa, Tatsuhiro Tomiyama, Koichi Kyono

Published in: Reproductive Medicine and Biology | Issue 1/2014

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Abstract

Purpose

The purpose of this study was to evaluate the effect of transportation at prolonged low temperatures on the survival of pre-antral follicles.

Methods

Ovarian tissue was removed from six women with gender identity disorder. Tissues were stored in an icebox at 4 °C for 6 or 18 h prior to vitrification. After warming, ovarian tissues were cultured for 24 h and follicle survival was assessed via a viability/cytotoxicity kit. Morphological features and oxygen consumption rate (OCR) were evaluated by scanning electrochemical microscopy (SECM).

Results

Survival rate of isolated primordial follicles was 95.7 and 100 %, and that of primary follicles was 91.7 and 81.8 % in the 6- and 18-h groups respectively. There was no difference in morphology between the 6- and 18-h storage groups. In comparison with OCR of vitrified-warmed follicles and OCR of 24-h culture after vitrified-warmed follicles, OCR of 24-h culture after vitrified-warmed primordial follicles was significantly higher in both 6-hour (0.02 ± 0.02 vs 0.07 ± 0.04, P < 0.05) and 18-h groups (0.02 ± 0.02 vs 0.11 ± 0.10, P < 0.05).

Conclusions

This strongly suggests that prolonged transportation of ovarian tissue at low temperatures is useful when there are no available local systems for fertility preservation.
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Metadata
Title
Evaluation of oxygen consumption in human vitrified and warmed pre-antral follicles after prolonged low temperatures
Authors
Toshihiko Kyoya
Yusuke Nakamura
Shizue Miyatani
Tomoko Miyagawa
Tatsuhiro Tomiyama
Koichi Kyono
Publication date
01-01-2014
Publisher
Springer Japan
Published in
Reproductive Medicine and Biology / Issue 1/2014
Print ISSN: 1445-5781
Electronic ISSN: 1447-0578
DOI
https://doi.org/10.1007/s12522-013-0166-9

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