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Indian Journal of Hematology and Blood Transfusion

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01-04-2021 | Acute Myeloid Leukemia | Original Article

Downregulation of MIR100HG Induces Apoptosis in Human Megakaryoblastic Leukemia Cells

Authors: Parisa Bagheri, Mohammadreza Sharifi, Ava Ghadiri

Published in: Indian Journal of Hematology and Blood Transfusion | Issue 2/2021

Abstract

Long noncoding ribonucleic acids (lncRNAs) are ribonucleic acid (RNA) molecules longer than 200 nucleotides without protein-coding capacity. Several studies have shown that lncRNAs play a pivotal role in the initiation, maintenance, and progression of acute myeloid leukemia (AML), which could make them a promising candidate in the diagnosis and treatment of leukemia. Acute Megakaryoblastic leukemia (AMKL) is a rare form of AML with a poor prognosis and low survival. It has been reported that lncRNA MIR100HG is involved several types of malignancies. In the present study, MIR100HG was downregulated in a human acute megakaryoblastic leukemia cell line (M-07e) using Antisense LNA GapmeRs. In order to assess the expression level of MIR100HG, cell viability, apoptosis, and necrosis (late apoptosis), quantitative reverse transcription polymerase chain reaction (qRT-PCR), Methyl-thiazol Tetrazolium assay, AnnexinV, and propidium iodide staining was performed at different time points after the transfection. In addition, the expression level of TGFβ was evaluated by qRT-PCR. Our results revealed that inhibition of MIR100HG might serve as a new method for inhibition of the proliferation of AMKL cells and therefore, could be a promising approach in medicine for targeted therapy in AMKL.

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Title: Downregulation of MIR100HG Induces Apoptosis in Human Megakaryoblastic Leukemia Cells
Authors: Parisa Bagheri
Mohammadreza Sharifi
Ava Ghadiri
Publication date: 01-04-2021
Publisher: Springer India
Keywords: Acute Myeloid Leukemia
Leukemia
Published in: Indian Journal of Hematology and Blood Transfusion / Issue 2/2021
Print ISSN: 0971-4502
Electronic ISSN: 0974-0449
DOI: https://doi.org/10.1007/s12288-020-01324-6

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