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Current Fungal Infection Reports
Published in: Current Fungal Infection Reports 4/2017

01-12-2017 | Clinical Mycology Lab Issues (S Córdoba, Section Editor)

A Comprehensive Analysis of MALDI-TOF MS and Ribosomal DNA Sequencing for Identification of Clinical Yeasts

Authors: Constanza Giselle Taverna, Mariana Mazza, Nicolas Refojo, Graciela Davel

Published in: Current Fungal Infection Reports | Issue 4/2017

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Abstract

Purpose of Review

The purpose of this study is to analyze the current knowledge about matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) identification of clinical yeasts comparing with the ribosomal DNA sequencing identification. We also review the lasts taxonomical and nomenclature changes of clinical yeasts.

Recent Findings

In most studies, the two main commercially available, MALDI Biotyper and VITEK MS, correctly identified more than 90% of yeasts isolates. Most drawbacks are due to low discrimination between closely related species or due to a lack of the reference main spectra (MSP) in the database. The addition of MSPs in the databases improves the performance.

Summary

MALDI-TOF MS has proven to be good for the identification of clinical yeasts and to differentiate some closely related species or species undifferentiated by conventional techniques. However, some species remain difficult to differentiate by this technique and should be identified by ribosomal DNA sequencing. Accurate and rapid yeasts identification could improve the management of patients with yeasts infections.
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Metadata
Title
A Comprehensive Analysis of MALDI-TOF MS and Ribosomal DNA Sequencing for Identification of Clinical Yeasts
Authors
Constanza Giselle Taverna
Mariana Mazza
Nicolas Refojo
Graciela Davel
Publication date
01-12-2017
Publisher
Springer US
Published in
Current Fungal Infection Reports / Issue 4/2017
Print ISSN: 1936-3761
Electronic ISSN: 1936-377X
DOI
https://doi.org/10.1007/s12281-017-0297-2

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