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01-09-2009 | Original Paper

Hematopoietic- and Neurologic-Expressed Sequence 1 Expression in the Murine GL261 and High-Grade Human Gliomas

Authors: Katharine M. Laughlin, Defang Luo, Che Liu, Gerry Shaw, Kenneth H. Warrington Jr., Jingxin Qiu, Anthony T. Yachnis, Jeffrey K. Harrison

Published in: Pathology & Oncology Research | Issue 3/2009

Abstract

The hematopoietic- and neurologic-expressed sequence 1 (Hn1) gene encodes a highly conserved protein that is expressed in developing and regenerating tissues. In this study, Hn1 expression was evaluated in human and murine malignant gliomas. Hn1 mRNA and protein were detected in the murine GL261 glioma cell line and in GL261 brain tumors in vivo. HN1 is also expressed in human U118MG and U87MG cell lines. Evaluation of human brain tumors using an anti-Hn1 polyclonal antibody detected strong immunoreactivity in high-grade (WHO III and IV) malignant gliomas. The rate of GL261 cell proliferation in vitro was unaltered by Hn1 depletion using an anti-Hn1 siRNA. However, tumors established from Hn1-depleted GL261 cells formed significantly smaller volumes than those established from control-treated cells. These data suggest a role for Hn1 in the biology of malignant brain tumors.

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Title: Hematopoietic- and Neurologic-Expressed Sequence 1 Expression in the Murine GL261 and High-Grade Human Gliomas
Authors: Katharine M. Laughlin
Defang Luo
Che Liu
Gerry Shaw
Kenneth H. Warrington Jr.
Jingxin Qiu
Anthony T. Yachnis
Jeffrey K. Harrison
Publication date: 01-09-2009
Publisher: Springer Netherlands
Published in: Pathology & Oncology Research / Issue 3/2009
Print ISSN: 1219-4956
Electronic ISSN: 1532-2807
DOI: https://doi.org/10.1007/s12253-008-9147-4

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