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Environmental Health and Preventive Medicine
Published in: Environmental Health and Preventive Medicine 3/2015

01-05-2015 | Regular Article

The effect of co-administration of Lawsonia inermis extract and octreotide on experimental hepatocellular carcinoma

Authors: N. M. Abdel-Hamid, O. M. Mohafez, M. H. Nazmy, A. Farhan, K. Thabet

Published in: Environmental Health and Preventive Medicine | Issue 3/2015

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Abstract

Objectives

To investigate the effect of Lawsonia inermis total methanolic extract (LIE) and octreotide (OC) on hepatocellular carcinoma (HCC) progression, depending on somatostatin receptor 2 (SSTR-2) and Alfa fetoprotein (AFP) perturbations.

Methods

Sixty albino mice, divided into five groups (12/each); all except control were injected with single diethyl nitrosamine (DENA) dose of 90 mg/kg body weight, intraperitoneally (IP). DENA group was killed at the last day of week 18. LIE group was given 200 mg/100 ml drinking water from first day of DENA injection until end of week 18. OC group received OC (0.1 mg/kg body weight, twice daily by subcutaneous injection, SC from the first day of week 17 till end of week 18. LIE + OC was given medications till the last day of week 18. Serum AFP, liver tissue SSTR-2 mRNA, its protein expression, reduced glutathione (GSH) and malondialdehyde (MDA) were analyzed.

Results

A significant increase in plasma AFP and hepatic mRNA, associated to liver tissue neoplastic changes, SSTR-2 expression and MDA with decreased hepatic GSH were observed in DENA group. These changes were significantly improved by LIE and/or OC.

Conclusions

LIE and/or OC treatment has effective chemopreventive action due to their ability to alleviate oxidative stress, desensitizing cellular growth receptor to SST.
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Metadata
Title
The effect of co-administration of Lawsonia inermis extract and octreotide on experimental hepatocellular carcinoma
Authors
N. M. Abdel-Hamid
O. M. Mohafez
M. H. Nazmy
A. Farhan
K. Thabet
Publication date
01-05-2015
Publisher
Springer Japan
Published in
Environmental Health and Preventive Medicine / Issue 3/2015
Print ISSN: 1342-078X
Electronic ISSN: 1347-4715
DOI
https://doi.org/10.1007/s12199-015-0451-9

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