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Annals of Nuclear Medicine
Published in: Annals of Nuclear Medicine 1/2023

Open Access 28-10-2022 | Positron Emission Tomography | Original Article

Detection of apoptosis by [18F]ML-10 after cardiac ischemia–reperfusion injury in mice

Authors: Maximilian Fischer, Mathias J. Zacherl, Jessica Olivier, Simon Lindner, Steffen Massberg, Peter Bartenstein, Freba Grawe, Sibylle Ziegler, Matthias Brendel, Sebastian Lehner, Guido Boening, Andrei Todica

Published in: Annals of Nuclear Medicine | Issue 1/2023

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Abstract

Objective

Myocardial infarction leads to ischemic heart disease and cell death, which is still a major obstacle in western society. In vivo imaging of apoptosis, a defined cascade of cell death, could identify myocardial tissue at risk.

Methods

Using 2-(5-[18F]fluoropentyl)-2-methyl-malonic acid ([18F]ML-10) in autoradiography and positron emission tomography (PET) visualized apoptosis in a mouse model of transient ligation of the left anterior descending (LAD) artery. 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) PET imaging indicated the defect area. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) histology stain indicated cardiac apoptosis.

Results

[18F]ML-10 uptake was evident in the ischemic area after transient LAD ligation in ex vivo autoradiography and in vivo PET imaging. Detection of [18F]ML-10 is in line with the defect visualized by [18F]FDG and the histological approach of TUNEL staining.

Conclusion

The tracer [18F]ML-10 is suitable for detecting apoptosis after transient LAD ligation in mice.
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Metadata
Title
Detection of apoptosis by [18F]ML-10 after cardiac ischemia–reperfusion injury in mice
Authors
Maximilian Fischer
Mathias J. Zacherl
Jessica Olivier
Simon Lindner
Steffen Massberg
Peter Bartenstein
Freba Grawe
Sibylle Ziegler
Matthias Brendel
Sebastian Lehner
Guido Boening
Andrei Todica
Publication date
28-10-2022
Publisher
Springer Nature Singapore
Published in
Annals of Nuclear Medicine / Issue 1/2023
Print ISSN: 0914-7187
Electronic ISSN: 1864-6433
DOI
https://doi.org/10.1007/s12149-022-01801-0

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