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Published in: Medical Oncology 1/2015

01-01-2015 | Original Paper

Texosome-anchored superantigen triggers apoptosis in original ovarian cancer cells

Authors: Hamideh Mahmoodzadeh Hosseini, Jafar Soleimanirad, Elnaz Mehdizadeh Aghdam, Mohsen Amin, Abbas Ali Imani Fooladi

Published in: Medical Oncology | Issue 1/2015

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Abstract

Texosomes, nano-endosomal vesicles, are candidates for cancer immunotherapy due to their immunostimulating properties. We designed a new structure based on texosome and staphylococcal enterotoxin B (SEB) and assessed its cytotoxic impact on an ovarian cell line. Texosomes were isolated from tumor cells, and SEB was anchored onto by protein transfer method. MTT assay and Hoechst staining were used to identify the cytotoxic and apoptotic effects of this compound on treated cells with different concentrations of texosome–SEB (TEX–SEB). Moreover, the expression rate of bcl-2, bax, bak, bcl-xl and the activity of caspase-3 and caspase-9 were investigated. Treatments of the cells with 0.5, 2.5 and 10 μg/100 μl TEX–SEB were significantly cytotoxic within 24 h (p < 0.001). Hoechst staining revealed that all tested concentrations caused apoptosis after 24 h compared with the control cells (p < 0.001). Furthermore, it was found that treatment with all examined concentrations of TEX–SEB enhanced caspase-9 activity after 24 and 48 h, while caspase-3 activity was increased upon treatment with only 0.5 and 2.5 μg/100 μl of TEX–SEB after 24 h (p < 0.001). None of the concentrations of TEX–SEB affected the expression of the cancer-promoting genes. Our construct, the TEX–SEB, is a new model being able to create cytostatic properties on cancer cells.
Literature
2.
go back to reference Alberts DS. Treatment of refractory and recurrent ovarian cancer. Semin Oncol. 1999;26(1 Suppl 1):8–14.PubMed Alberts DS. Treatment of refractory and recurrent ovarian cancer. Semin Oncol. 1999;26(1 Suppl 1):8–14.PubMed
3.
go back to reference McGuire WP, Ozols RF. Chemotherapy of advanced ovarian cancer. Semin Oncol. 1998;25(3):340–8.PubMed McGuire WP, Ozols RF. Chemotherapy of advanced ovarian cancer. Semin Oncol. 1998;25(3):340–8.PubMed
4.
go back to reference Rieger J, Freichels H, Imberty A, Putaux JL, Delair T, Jérôme C, et al. Polyester nanoparticles presenting mannose residues: toward the development of new vaccine delivery systems combining biodegradability and targeting properties. Biomacromolecules. 2009;10(3):651–7. doi:10.1021/bm801492c.PubMedCrossRef Rieger J, Freichels H, Imberty A, Putaux JL, Delair T, Jérôme C, et al. Polyester nanoparticles presenting mannose residues: toward the development of new vaccine delivery systems combining biodegradability and targeting properties. Biomacromolecules. 2009;10(3):651–7. doi:10.​1021/​bm801492c.PubMedCrossRef
5.
go back to reference Hosseini HM, Fooladi AA, Nourani MR, Ghanezadeh F. Role of Exosome in infectious disease. Inflamm Allergy Drug Targets. 2013;12(1):29–37.PubMedCrossRef Hosseini HM, Fooladi AA, Nourani MR, Ghanezadeh F. Role of Exosome in infectious disease. Inflamm Allergy Drug Targets. 2013;12(1):29–37.PubMedCrossRef
8.
go back to reference Tan A, De La Peña H, Seifalian AM. The application of exosomes as a nanoscale cancer vaccine. Int J Nanomed. 2010;5:889–900. doi:10.2147/ijn.s13402. Tan A, De La Peña H, Seifalian AM. The application of exosomes as a nanoscale cancer vaccine. Int J Nanomed. 2010;5:889–900. doi:10.​2147/​ijn.​s13402.
9.
go back to reference Ristorcelli E, Beraud E, Mathieu S, Lombardo D, Verine A. Essential role of Notch signaling in apoptosis of human pancreatic tumoral cells mediated by exosomal nanoparticles. Int J Cancer. 2009;125(5):1016–26. doi:10.1002/ijc.24375.PubMedCrossRef Ristorcelli E, Beraud E, Mathieu S, Lombardo D, Verine A. Essential role of Notch signaling in apoptosis of human pancreatic tumoral cells mediated by exosomal nanoparticles. Int J Cancer. 2009;125(5):1016–26. doi:10.​1002/​ijc.​24375.PubMedCrossRef
11.
go back to reference Mahmoodzadeh Hosseini H, Ali Imani Fooladi A, Soleimanirad J, Reza Nourani M, Mahdavi M. Exosome/staphylococcal enterotoxin B, an anti tumor compound against pancreatic cancer. J BUON. 2014;19(2):440–8.PubMed Mahmoodzadeh Hosseini H, Ali Imani Fooladi A, Soleimanirad J, Reza Nourani M, Mahdavi M. Exosome/staphylococcal enterotoxin B, an anti tumor compound against pancreatic cancer. J BUON. 2014;19(2):440–8.PubMed
12.
go back to reference Mahmoodzadeh Hosseini H, Imani Fooladi AA, Soleimanirad J, Nourani MR, Davaran S, Mahdavi M. Staphylococcal entorotoxin B anchored exosome induces apoptosis in negative estrogen receptor breast cancer cells. Tumour Biol. 2014;35(4):3699–707. doi:10.1007/s13277-013-1489-1.PubMedCrossRef Mahmoodzadeh Hosseini H, Imani Fooladi AA, Soleimanirad J, Nourani MR, Davaran S, Mahdavi M. Staphylococcal entorotoxin B anchored exosome induces apoptosis in negative estrogen receptor breast cancer cells. Tumour Biol. 2014;35(4):3699–707. doi:10.​1007/​s13277-013-1489-1.PubMedCrossRef
13.
go back to reference Choi YW, Kotzin B, Herron L, Callahan J, Marrack P, Kappler J. Interaction of Staphylococcus aureus toxin “superantigens” with human T cells. Proc Natl Acad Sci. 1989;86(22):8941–5.PubMedCentralPubMedCrossRef Choi YW, Kotzin B, Herron L, Callahan J, Marrack P, Kappler J. Interaction of Staphylococcus aureus toxin “superantigens” with human T cells. Proc Natl Acad Sci. 1989;86(22):8941–5.PubMedCentralPubMedCrossRef
14.
go back to reference Kappler J, Kotzin B, Herron L, Gelfand EW, Bigler RD, Boylston A, et al. V beta-specific stimulation of human T cells by staphylococcal toxins. Science. 1989;244(4906):811–3.PubMedCrossRef Kappler J, Kotzin B, Herron L, Gelfand EW, Bigler RD, Boylston A, et al. V beta-specific stimulation of human T cells by staphylococcal toxins. Science. 1989;244(4906):811–3.PubMedCrossRef
15.
go back to reference Imani Fooladi AA, Sattari M, Hassan ZM, Mahdavi M, Azizi T, Horii A. In vivo induction of necrosis in mice fibrosarcoma via intravenous injection of type B staphylococcal enterotoxin. Biotechnol Lett. 2008;30(12):2053–9. doi:10.1007/s10529-008-9805-3.CrossRef Imani Fooladi AA, Sattari M, Hassan ZM, Mahdavi M, Azizi T, Horii A. In vivo induction of necrosis in mice fibrosarcoma via intravenous injection of type B staphylococcal enterotoxin. Biotechnol Lett. 2008;30(12):2053–9. doi:10.​1007/​s10529-008-9805-3.CrossRef
16.
go back to reference Imani Fooladi AA, Sattari M, Nourani MR. Synergistic effects between Staphylococcal enterotoxin type B and monophosphoryl lipid A against mouse fibrosarcoma. J BUON. 2010;15(2):340–7.PubMed Imani Fooladi AA, Sattari M, Nourani MR. Synergistic effects between Staphylococcal enterotoxin type B and monophosphoryl lipid A against mouse fibrosarcoma. J BUON. 2010;15(2):340–7.PubMed
17.
go back to reference Fooladi AAI, Sattari M, Nourani MR. Study of T-cell stimulation and cytokine release induced by Staphylococcal enterotoxin type B and monophosphoryl lipid A. Arch Med Sci. 2009;3:335–41. Fooladi AAI, Sattari M, Nourani MR. Study of T-cell stimulation and cytokine release induced by Staphylococcal enterotoxin type B and monophosphoryl lipid A. Arch Med Sci. 2009;3:335–41.
19.
go back to reference Battke C, Ruiss R, Welsch U, Wimberger P, Lang S, Jochum S, et al. Tumour exosomes inhibit binding of tumour-reactive antibodies to tumour cells and reduce ADCC. Cancer Immunol Immunother. 2011;60(5):639–48. doi:10.1007/s00262-011-0979-5.PubMedCrossRef Battke C, Ruiss R, Welsch U, Wimberger P, Lang S, Jochum S, et al. Tumour exosomes inhibit binding of tumour-reactive antibodies to tumour cells and reduce ADCC. Cancer Immunol Immunother. 2011;60(5):639–48. doi:10.​1007/​s00262-011-0979-5.PubMedCrossRef
20.
go back to reference McHugh RS, Nagarajan S, Wang YC, Sell KW, Selvaraj P. Protein transfer of glycosyl-phosphatidylinositol-B7-1 into tumor cell membranes: a novel approach to tumor immunotherapy. Cancer Res. 1999;59(10):2433–7.PubMed McHugh RS, Nagarajan S, Wang YC, Sell KW, Selvaraj P. Protein transfer of glycosyl-phosphatidylinositol-B7-1 into tumor cell membranes: a novel approach to tumor immunotherapy. Cancer Res. 1999;59(10):2433–7.PubMed
22.
go back to reference Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ. Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes. J Biol Chem. 1998;273(32):20121–7.PubMedCrossRef Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ. Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes. J Biol Chem. 1998;273(32):20121–7.PubMedCrossRef
23.
go back to reference Wubbolts R, Leckie RS, Veenhuizen PT, Schwarzmann G, Möbius W, Hoernschemeyer J, et al. Proteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation. J Biol Chem. 2003;278(13):10963–72. doi:10.1074/jbc.M207550200.PubMedCrossRef Wubbolts R, Leckie RS, Veenhuizen PT, Schwarzmann G, Möbius W, Hoernschemeyer J, et al. Proteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation. J Biol Chem. 2003;278(13):10963–72. doi:10.​1074/​jbc.​M207550200.PubMedCrossRef
24.
25.
go back to reference Nagarajan S, Anderson M, Ahmed SN, Sell KW, Selvaraj P. Purification and optimization of functional reconstitution on the surface of leukemic cell lines of GPI-anchored Fc gamma receptor III. J Immunol Methods. 1995;184(2):241–51.PubMedCrossRef Nagarajan S, Anderson M, Ahmed SN, Sell KW, Selvaraj P. Purification and optimization of functional reconstitution on the surface of leukemic cell lines of GPI-anchored Fc gamma receptor III. J Immunol Methods. 1995;184(2):241–51.PubMedCrossRef
27.
go back to reference Sharief M, Gani ZH. Garden cress lepidium sativum seeds as oral contraceptive plant in mice. Saudi Med J. 2004;25(7):965–6.PubMed Sharief M, Gani ZH. Garden cress lepidium sativum seeds as oral contraceptive plant in mice. Saudi Med J. 2004;25(7):965–6.PubMed
28.
go back to reference Ferrari D, Pinton P, Campanella M, Callegari MG, Pizzirani C, Rimessi A, et al. Functional and structural alterations in the endoplasmic reticulum and mitochondria during apoptosis triggered by C2-ceramide and CD95/APO-1/FAS receptor stimulation. Biochem Biophys Res Commun. 2010;391(1):575–81. doi:10.1016/j.bbrc.2009.11.101.PubMedCrossRef Ferrari D, Pinton P, Campanella M, Callegari MG, Pizzirani C, Rimessi A, et al. Functional and structural alterations in the endoplasmic reticulum and mitochondria during apoptosis triggered by C2-ceramide and CD95/APO-1/FAS receptor stimulation. Biochem Biophys Res Commun. 2010;391(1):575–81. doi:10.​1016/​j.​bbrc.​2009.​11.​101.PubMedCrossRef
Metadata
Title
Texosome-anchored superantigen triggers apoptosis in original ovarian cancer cells
Authors
Hamideh Mahmoodzadeh Hosseini
Jafar Soleimanirad
Elnaz Mehdizadeh Aghdam
Mohsen Amin
Abbas Ali Imani Fooladi
Publication date
01-01-2015
Publisher
Springer US
Published in
Medical Oncology / Issue 1/2015
Print ISSN: 1357-0560
Electronic ISSN: 1559-131X
DOI
https://doi.org/10.1007/s12032-014-0409-6

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