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Medical Oncology
Published in: Medical Oncology 1/2013

01-03-2013 | Original Paper

The effect of Bcl-2 siRNA combined with miR-15a oligonucleotides on the growth of Raji cells

Authors: Xiaomao Hu, Wenyu Li, Gexiu Liu, Hong Wu, Yangjun Gao, Shengting Chen, Dongmei He, Yuan Zhang

Published in: Medical Oncology | Issue 1/2013

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Abstract

Bcl-2 is a prominent member of the Bcl-2 family of proteins that regulate the induction of apoptosis. Here, we investigated the effect of Bcl-2 small interfering RNA (siRNA) combined with miR-15a oligonucleotides (ODN) on the growth of Raji cells. The expression levels of Bcl-2 mRNA and protein were detected. The inhibition of cell growth and apoptosis was determined. Atomic force microscopy was utilized to observe the cell-surface ultrastructural changes. After transfection of combination, protein and mRNA levels of Bcl-2 obviously were decreased. The growth of cells was significantly inhibited compared with those cells transfected with Bcl-2 siRNA or miR-15a alone. Apoptotic rate significantly increased. The cells of apoptosis showed a roughness variation on cell membranes. These results suggest that the combination of Bcl-2 siRNA and miR-15a ODN increases apoptosis in Raji cells. Our study implies that the combination of Bcl-2 siRNA and miR-15a may be a useful approach in treatment for lymphoma.
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Metadata
Title
The effect of Bcl-2 siRNA combined with miR-15a oligonucleotides on the growth of Raji cells
Authors
Xiaomao Hu
Wenyu Li
Gexiu Liu
Hong Wu
Yangjun Gao
Shengting Chen
Dongmei He
Yuan Zhang
Publication date
01-03-2013
Publisher
Springer US
Published in
Medical Oncology / Issue 1/2013
Print ISSN: 1357-0560
Electronic ISSN: 1559-131X
DOI
https://doi.org/10.1007/s12032-012-0430-6

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