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01-12-2011 | Original paper

Induction of lung epithelial cell transformation and fibroblast activation by Yunnan tin mine dust and their interaction

Authors: Li Bian, Yong-Wen He, Rui-Zhu Tang, Li-Ju Ma, Chun-Yan Wang, Yong-Hua Ruan, Qian Gao, Ke-Wei Jin

Published in: Medical Oncology | Special Issue 1/2011

Abstract

Tumor–stroma interactions play a significant role in tumor development and progression. Our study employed an in vitro co-culture model of epithelial cells and fibroblasts to investigate the mechanism of and interaction between lung epithelial cell transformation and fibroblast activation induced by Yunnan tin mine dust. Epithelial cell transformation was evaluated using concanavalin A agglutination and anchorage-independent growth assays, and fibroblast activation was assessed via immunohistochemistry. The TGF-β1/Smad pathway was monitored by Western blot analysis and ELISA. We found concanavalin A agglutination and anchorage-independent growth assays of dust-exposed epithelial cells were positive, dust-exposed fibroblasts expressed α-SMA, and during the mine dust-induced tumorigenesis, TGF-β1/Smad signaling pathway changed. In conclusion, Yunnan tin mine dust is able to induce the malignant transformation of bronchial epithelial cells and fibroblast activation. Epithelial cells are the main target of mine dust. Bronchial epithelial cell transformation and fibroblast activation are correlated and synergistic. Their interdependence is related to the TGF-β1/Smad signaling pathway.

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Title: Induction of lung epithelial cell transformation and fibroblast activation by Yunnan tin mine dust and their interaction
Authors: Li Bian
Yong-Wen He
Rui-Zhu Tang
Li-Ju Ma
Chun-Yan Wang
Yong-Hua Ruan
Qian Gao
Ke-Wei Jin
Publication date: 01-12-2011
Publisher: Springer US
Published in: Medical Oncology / Issue Special Issue 1/2011
Print ISSN: 1357-0560
Electronic ISSN: 1559-131X
DOI: https://doi.org/10.1007/s12032-010-9655-4

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